Development of analytical kit to prevent licorice-induced pseudoaldsteronism

• Project/Area Number: 25460907
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General internal medicine(including psychosomatic medicine)
• Research Institution: Nagoya City University
• Principal Investigator: Makino Toshiaki 名古屋市立大学, 薬学研究科(研究院), 教授 (80326561)
• Co-Investigator(Renkei-kenkyūsha): Morinaga Osamu 第一薬科大学, 薬学部, 准教授 (60465771)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 漢方薬 / 副作用 / 偽アルドステロン症 / トランスポーター / グリチルリチン / 甘草

Outline of Final Research Achievements

Licorice-induced pseudoaldosteronism is the most frequent side-effect of Kampo medicine. I expected that 3-monoglucronyl-glycyrrhetinic acid (3MGA) is the genuine causative metabolite of glycyrrhetinic acid to cause pseudoaldosteronism. The aim of this study is to evaluate the effectiveness of anti-3MGA antibody to measure the concentration of 3MGA in serum and urine collected from GA-treated Mrp2-deficient rats. However, I found the cross-reactivity of anti-3MGA antibody with unknown metabolite of GA in serum and urine of Mrp2-deficient rats. I isolated this unknown metabolite from the urine and determined its structure. This compound might be the genuine causative metabolite for licorice-induced pseudoaldosteronism instead of 3MGA.

Research Products

• [Journal Article] 3-Monoglucuronyl Glycyrrhretinic Acid Is a Possible Marker Compound Related to Licorice-Induced Pseudoaldosteronism (2014)
  • Author(s): Toshiaki Makino
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 37 Issue: 6 Pages: 898-902
  • DOI: 10.1248/bpb.b13-00997
  • NAID: 130004147345
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access
• [Book] いまさら聞けない生薬・漢方薬 (2015)
  • Author(s): 牧野利明
  • Total Pages: 195
  • Publisher: 医薬経済社
• [Book] Traditional Medicine – New Research (2013)
  • Author(s): Toshiaki Makino, Nobuhiro Ohtake
  • Total Pages: 274
  • Publisher: Nova Science Publishers