Development of analytical kit to prevent licorice-induced pseudoaldsteronism
| Project/Area Number |
25460907
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General internal medicine(including psychosomatic medicine)
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| Research Institution | Nagoya City University |
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Principal Investigator |
Makino Toshiaki 名古屋市立大学, 薬学研究科(研究院), 教授 (80326561)
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| Co-Investigator(Renkei-kenkyūsha) |
Morinaga Osamu 第一薬科大学, 薬学部, 准教授 (60465771)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 漢方薬 / 副作用 / 偽アルドステロン症 / トランスポーター / グリチルリチン / 甘草 |
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| Outline of Final Research Achievements |
Licorice-induced pseudoaldosteronism is the most frequent side-effect of Kampo medicine. I expected that 3-monoglucronyl-glycyrrhetinic acid (3MGA) is the genuine causative metabolite of glycyrrhetinic acid to cause pseudoaldosteronism. The aim of this study is to evaluate the effectiveness of anti-3MGA antibody to measure the concentration of 3MGA in serum and urine collected from GA-treated Mrp2-deficient rats. However, I found the cross-reactivity of anti-3MGA antibody with unknown metabolite of GA in serum and urine of Mrp2-deficient rats. I isolated this unknown metabolite from the urine and determined its structure. This compound might be the genuine causative metabolite for licorice-induced pseudoaldosteronism instead of 3MGA.
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Report
(4 results)
Research Products
(3 results)
