The morphology of small intestine and the restorative effect on drug induced colitis in proglucagon derived peptides knockout mice

Research Project

Project/Area Number	25460949
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Gastroenterology
Research Institution	Nagoya University
Principal Investigator	Ando Takafumi 名古屋大学, 医学系研究科, 招へい教員 (80378041)
Co-Investigator(Kenkyū-buntansha)	後藤秀実名古屋大学, 医学系研究科, 教授 (10215501) 前田修名古屋大学, 医学系研究科, 特任講師 (20378053) 渡辺修名古屋大学, 医学部附属病院, 病院講師 (80378059) 尾崎信暁名古屋大学, 総合保健体育科学センター, 特任教授 (70378082) 大宮直木藤田保健衛生大学, 医学部, 教授 (00335035)
Research Collaborator	Hayashi Yoshitaka 名古屋大学, 環境医学研究所, 准教授
Project Period (FY)	2013-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	GLP-2 / DSS腸炎 / 腸管L細胞 / GLP-2欠損マウス / 体重減少 / Gcggfp/gfp （KO）マウス / 絨毛 / DSS腸炎の改善程度 / グルカゴン遺伝子欠損マウス(GcgKO) / GLP-1 / DPP-4 阻害薬
Outline of Final Research Achievements	We elucidated the morphology of small intestine and the restoration on colitis with deficiency of proglucagon derived peptides (PGDPs) containing GLP-2. Mice deficient with PGDPs were used. Eight-week-old male mice of PGDPs-/- and PGDPs+/+ were sacrificed to check the morphology of the small intestine. To elucidate the effect of the deletion of PGDPs on colitis, 12-week-old PGDPs -/- and PGDPs+/+ male mice were exposed to 2.5% dextran sulfate sodium (DSS) for 6 days. Severity of colitis was assessed daily by body weight loss and disease activity index. Histological examinations were performed on day 7 and 21. There were no deformity and no differences in the villous height and crypt depth in PGDPs-/- mice. In DSS colitis, the decline and amelioration of body weight and disease activity index were not different between PGDP-/- and PGDP+/+.PGDPs deletion did not showed the morphological deformity of small intestine and differences in colitis.