| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The cause of ulcerative colitis is unclear. Because the next-generation sequencer can determine nucleotide sequence without needing known base sequence information, it is used for identification of unknown pathogenic viruses. In this study, an unknown virus is assumed as the etiology of ulcerative colitis and was intended to identify this.
Colonic mucosal samples were obtained from patients with ulcerative colitis. Nucleotide sequence was determined with a next-generation sequencer. Human and bacterial nucleotide sequence was removed and virus nucleotide sequence was extracted on homologous analysis. More than 300 virus sequences per 1 sample were identified. The most frequently identified sequences were those similar to Human endogenous retrovirus, and Stealth virus.
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