Identification of the pathogenic virus of ulcerative colitis using the next-generation sequencing techonology
Project/Area Number |
25460961
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
|
Research Institution | Tokyo Medical and Dental University (2014-2015) Keio University (2013) |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 潰瘍性大腸炎 / 次世代シーケンサー / ウイルス / 病原ウイルス / 未知ウイルス |
Outline of Final Research Achievements |
The cause of ulcerative colitis is unclear. Because the next-generation sequencer can determine nucleotide sequence without needing known base sequence information, it is used for identification of unknown pathogenic viruses. In this study, an unknown virus is assumed as the etiology of ulcerative colitis and was intended to identify this. Colonic mucosal samples were obtained from patients with ulcerative colitis. Nucleotide sequence was determined with a next-generation sequencer. Human and bacterial nucleotide sequence was removed and virus nucleotide sequence was extracted on homologous analysis. More than 300 virus sequences per 1 sample were identified. The most frequently identified sequences were those similar to Human endogenous retrovirus, and Stealth virus.
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Report
(4 results)
Research Products
(2 results)
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[Journal Article] Ulcerative Colitis Endoscopic Index of Severity Is Associated with Long-term Prognosis in Ulcerative Colitis Patients Treated with Infliximab2016
Author(s)
Keiichiro Saigusa, Katsuyoshi Matsuoka, Shinya Sugimoto, Mari Arai, Hiroki Kiyohara, Kozue Takeshita, Shinta Mizuno, Kiyoto Mori, Kosaku Nanki, Tatsuya Takeshita, Yoshihiro Nakazato, Tomoharu Yajima, Makoto Naganuma, Tadakazu Hisamatsu, Haruhiko Ogata, Yasushi Iwao, Takanori Kanai
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Journal Title
Digestive Endsoscopy
Volume: 印刷中
Issue: 6
Pages: 665-670
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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