Discovery and development of agents for chronic hepatitis C from marine natural products
Project/Area Number |
25460985
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | University of Yamanashi |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
ENOMOTO Nobuyuki 山梨大学, 総合研究部, 教授 (20251530)
MORIISHI Koji 山梨大学, 総合研究部, 教授 (90260273)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | C型肝炎ウイルス / 治療薬 / 海洋生物 / 抗ウイルス剤 / HCV / 治療薬開発 / 創薬 |
Outline of Final Research Achievements |
We focused on pharmacologically active component from marine organisms for the development of novel anti hepatitis C virus agent. Thus, we screened extracts of marine organisms by using HCV replicon system and showed that the extract from Amphimedon sp. inhibits HCV replication by suppressing viral helicase and protease activities. In this study, we attempted to purify and identify the active component of Amphimedon sp., but not purify and identify it. Additionally, we established a cell-based screening system in order to identify compounds inhibiting the HCV NS2/3 protease activity. We screened extracts of marine organisms by using this system, but not identify extracts of marine organisms inhibiting the HCV NS2/3 protease activity.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Identification of hydroxyanthraquinones as novel inhibitors of hepatitis C virus helicase2015
Author(s)
Atsushi Furuta, Masayoshi Tsubuki, Miduki Endoh, Tatsuki Miyamoto, Junichi Tanaka, Kazi Abdus Salam, Nobuyoshi Akimitsu, Hidenori Tani, Atsuya Yamashita, Kohji Moriishi, Masamichi Nakakoshi, Yuji Sekiguchi, Satoshi Tsuneda, Naohiro Noda
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Journal Title
International Journal of Molecular Sciences
Volume: 16
Issue: 8
Pages: 18439-18453
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Deep sequencing and phylogenetic analysis of variants resistant to interferon-based protease inhibitor therapy in chronic hepatitis induced by genotype 1b hepatitis C virus.2015
Author(s)
Sato M, Maekawa S, Komatsu N, Tatsumi A, Miura M, Muraoka M, Suzuki Y, Amemiya F, Takano S, Fukasawa M, Nakayama Y, Yamaguchi T, Uetake T, Inoue T, Sato T, Sakamoto M, Yamashita A, Moriishi K, Enomoto N.
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Journal Title
J Virol
Volume: 89(11)
Issue: 11
Pages: 6105-16
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of antiviral agents targeting hepatitis B virus promoter from extracts of Indonesian marine organisms by a novel cell-based screening assay.2015
Author(s)
Yamashita A, Fujimoto Y, Tamaki M, Setiawan A, Tanaka T, Okuyama-Dobashi K, Kasai H, Watashi K, Wakita T, Toyama M, Baba M, de Voogd NJ, Maekawa S, Enomoto N, Tanaka J, Moriishi K
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Journal Title
Marine Drug
Volume: 13
Issue: 11
Pages: 6759-6773
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Involvement of FKBP6 in hepatitis C virus replication.2015
Author(s)
Kasai H, Kawakami Y, Yokoe H, Yoshimura K, Matsuda M, Yasumoto J, Maekawa S, Yamashita A, Tanaka T, Ikeda M, Kato N, Okamoto T, Matsuura Y, Sakamoto N, Enomoto N, Takeda S, Fujii H, Tsubuki M, Kusunoki M, Moriishi K.
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Journal Title
Scientific reports
Volume: 5
Issue: 1
Pages: 16699-16699
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Hepatitis B virus efficiently infects non-adherent hepatoma cells via human sodium taurocholate cotransporting polypeptide.2015
Author(s)
Okuyama-Dobashi K, Kasai H, Tanaka T, Yamashita A, Yasumoto J, Chen W, Okamoto T, Maekawa S, Watashi K, Wakita T, Ryo A, Suzuki T, Matsuura Y, Enomoto N, Moriishi K.
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Journal Title
Scientific reports
Volume: 5
Issue: 1
Pages: 17047-17047
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Hallmarks of hepatitis C virus in equine hepacivirus.2015
Author(s)
Tanaka T, Kasai H, Yamashita A, Okuyama-Dobashi K, Yasumoto J, Maekawa S, Enomoto N, Okamoto T, Matsuura Y, Morimatsu M, Manabe N, Ochiai K, Yamashita K, Moriishi K.
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Journal Title
J Virol.
Volume: 88(22)
Issue: 22
Pages: 13352-66
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] PBDE: Structure-Activity Studies for the Inhibition of Hepatitis C Virus NS3 Helicase.2014
Author(s)
Salam KA, Furuta A, Noda N, Tsuneda S, Sekiguchi Y, Yamashita A, Moriishi K, Nakakoshi M, Tani H, Roy SR, Tanaka J, Tsubuki M, Akimitsu N
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Journal Title
Molecules
Volume: 19
Issue: 4
Pages: 4006-4020
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Identification and biochemical characterization of halisulfate 3 and suvanine as novel inhibitors of hepatitis C virus NS3 helicase from a marine sponge.2014
Author(s)
Furuta A, Salam KA, Hermawan I, Akimitsu N, Tanaka J, Tani H, Yamashita A, Moriishi K, Nakakoshi M, Tsubuki M, Peng PW, Suzuki Y, Yamamoto N, Sekiguchi Y, Tsuneda S, Noda N
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Journal Title
Marine Drugs
Volume: 12
Issue: 1
Pages: 462-476
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Deep-sequencing analysis of the association between the quasispecies nature of the hepatitis C virus core region and disease progression.2013
Author(s)
Miura M, Maekawa S, Takano S, Komatsu N, Tatsumi A, Asakawa Y, Shindo K, Amemiya F, Nakayama Y, Inoue T, Sakamoto M, Yamashita A, Moriishi K, Enomoto N.
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Journal Title
J Virol.
Volume: 87(23)
Issue: 23
Pages: 12541-12551
DOI
Related Report
Peer Reviewed
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[Journal Article] Psammaplin A inhibits hepatitis C virus NS3 helicase2013
Author(s)
Salam, K.A.; Furuta, A.; Noda, N.; Tsuneda, S.; Sekiguchi, Y.; Yamashita, A.; Moriishi, K.; Nakakoshi, M.; Tsubuki, M.; Tani, H.; Tanaka, J.; Akimitsu, N
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Journal Title
J. Nat. Med. in press
Volume: 67
Issue: 4
Pages: 765-772
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Serum RANTES level influences the response to pegylated interferon and ribavirin therapy in chronic hepatitis C.2013
Author(s)
Komase K, Maekawa S, Miura M, Sueki R, Kadokura M, Shindo H, Shindo K, Amemiya F, Nakayama Y, Inoue T, Sakamoto M, Yamashita A, Moriishi K, Enomoto N
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Journal Title
Hepatol Res.
Volume: 43(8)
Issue: 8
Pages: 865-875
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] 海綿由来セキステルペン系化合物metachromin AのB型肝炎ウイルスコアプロモーター抑制活性を介した抗ウイルス活性.2015
Author(s)
山下篤哉, 田中智久, 土橋香織, 葛西宏威, 渡士幸一, 脇田隆字, 外山正明, 馬場昌範, 前川伸哉, 榎本信幸, 玉城麻友美, 田中淳一, 森石恆司
Organizer
第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
Place of Presentation
神戸ポートアイランド(兵庫県神戸市)
Year and Date
2015-12-01
Related Report
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[Presentation] Metachromin A, sesquiterpene from marine sponge Dactylospongia metachromia, exerts anti-HBV activity via inhibition of HBV core promoter.2015
Author(s)
Atsuya Yamashita, Tomohisa Tanaka, Kaori Okuyama-Dobashi, Hirotake Kasai, Koichi Watashi, Takaji Wakita, Masaaki Toyama, Masanori Baba, Kohji Moriishi
Organizer
第63回日本ウイルス学会学術集会
Place of Presentation
福岡国際会議場(福岡県福岡市)
Year and Date
2015-11-22
Related Report
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[Presentation] カイメン由来化合物metachromin A及びその類縁化合物の抗HBVの検討2015
Author(s)
山下篤哉, 田中智久, 葛西宏威, 渡士幸一, 脇田隆字, 外山正明, 馬場昌範, 前川伸哉, 榎本信幸, 玉城麻友美, 田中 淳一, 森石恆司
Organizer
第25回抗ウイルス療法学会
Place of Presentation
国立感染症研究所(東京都新宿区)
Year and Date
2015-05-22
Related Report
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