| Project/Area Number |
25461191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kochi University |
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Principal Investigator |
KUBOTA TETSUYA 高知大学, 教育研究部医療学系臨床医学部門, 准教授 (30274377)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Akihito 高知大学, 教育研究部医療学系臨床医学部門, 教授 (30191513)
OHNISHI Hiroshi 高知大学, 教育研究部医療学系臨床医学部門, 助教 (90553876)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAI Mizu 高知大学, 教育研究部医療学系臨床医学部門, 助教 (40403886)
KAWASE Shigeo 高知大学, 教育研究部医療学系臨床医学部門, 助教 (80642058)
MIYAMOTO Shintaro 高知大学, 教育研究部医療学系臨床医学部門, 助教 (30633995)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | KL-6 / MUC1 / 間質性肺炎 / 肺障害 / 動物モデル / バイオマーカー |
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| Outline of Final Research Achievements |
KL-6, an epitope of MUC1 expressed mainly on type II pneumocytes in humans, is a sensitive marker for interstitial pneumonia. However, an in vivo model for KL-6 has not been established because no KL-6 epitope is expressed in animals other than humans and apes. To investigate whether KL-6 is detectable in human MUC1 transgenic mice and whether KL-6 level reflects the degree of lung injury, we examined levels of KL-6 and other biomarkers in a variety of lung injury models. KL-6 was expressed on type II pneumocytes and bronchiolar epithelial cells in naive hMUC1-Tg mice. Serum KL-6 levels in these mice were comparable to those in humans, and KL-6 levels in BALF were significantly higher than those in sera. Levels of KL-6 dramatically changed depending on the variety and severity of lung injuries, suggesting its function as a biomarker in hMUC1-Tg mice. KL-6 behaved differently from other biomarkers. This hMUC1-Tg mouse can be used for assessment of KL-6 in vivo during lung injury.
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