Establishment of a specific differentiation induction method to the kidneys constituent cells using a cell memory of the human kidney-derived iPS cells
| Project/Area Number |
25461208
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
TAKASE OSAMU 東京大学, 医学部附属病院, 講師 (60265684)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HISHIKAWA Keiichi 東京大学, 医学部附属病院, 准教授 (50255460)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FUJITA Toshiro 東京大学, 先端科学技術研究センター, 教授 (10114125)
NAKAUCHI Hiromitsu 東京大学, 医科学研究所, 教授 (40175485)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | Epigenetic Memory / 腎臓由来iPS細胞 / 腎系統特異的分化誘導法 / 包括的メチル化解析 / 網羅的マイクロアレイ解析 / 新規腎臓発生・再生因子 / Epigenetics / DNAメチル化解析 / 腎臓細胞記憶 / HDAC阻害剤 / エピジェネティックス / iPS細胞移植 / 腎臓再生 / 包括的DNAメチル化解析 |
|---|
| Outline of Final Research Achievements |
In this study, we have investigated the difference between fibroblast-derived iPS cells and the kidney-derived iPS cells by two kind renal lineage-specific differentiation induction methods. Both kidney-derived iPS cells by two induction methods became clear that easy to differentiate into kidney system. Also by comprehensive methylation analysis of each iPS cell, as a new renal lineage differentiation induction-related gene, hypermethylation group of 56 genes, and hypomethylation group of 17 genes were identified. In additional data of the comprehensive microarray analysis, it was narrowed down them to 8 genes. These genes are expected to be a kidney development and regeneration factor. We hope that it is useful in the future of the various studies.
|
Report
(4 results)
Research Products
(27 results)
-
-
-
[Journal Article] Immunomodulation with eicosapentaenoic acid supports the treatment of autoimmune small-vessel vasculitis.2014
Author(s)
Hirahashi J, Kawahata K, Arita M, Iwamoto R, Hishikawa K, Honda M, Hamasaki Y, Tanaka M, Okubo K, Kurosawa M, Takase O, Nakakuki M, Saiga K, Suzuki K, Kawachi S, Tojo A, Seki G, Marumo T, Hayashi M, Fujita T.
-
Journal Title
Sci. Rep
Volume: 4
Issue: 1
Pages: 6406-6406
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
