| Project/Area Number |
25461208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKASE OSAMU 東京大学, 医学部附属病院, 講師 (60265684)
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| Co-Investigator(Kenkyū-buntansha) |
HISHIKAWA Keiichi 東京大学, 医学部附属病院, 准教授 (50255460)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJITA Toshiro 東京大学, 先端科学技術研究センター, 教授 (10114125)
NAKAUCHI Hiromitsu 東京大学, 医科学研究所, 教授 (40175485)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Epigenetic Memory / 腎臓由来iPS細胞 / 腎系統特異的分化誘導法 / 包括的メチル化解析 / 網羅的マイクロアレイ解析 / 新規腎臓発生・再生因子 / Epigenetics / DNAメチル化解析 / 腎臓細胞記憶 / HDAC阻害剤 / エピジェネティックス / iPS細胞移植 / 腎臓再生 / 包括的DNAメチル化解析 |
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| Outline of Final Research Achievements |
In this study, we have investigated the difference between fibroblast-derived iPS cells and the kidney-derived iPS cells by two kind renal lineage-specific differentiation induction methods. Both kidney-derived iPS cells by two induction methods became clear that easy to differentiate into kidney system. Also by comprehensive methylation analysis of each iPS cell, as a new renal lineage differentiation induction-related gene, hypermethylation group of 56 genes, and hypomethylation group of 17 genes were identified. In additional data of the comprehensive microarray analysis, it was narrowed down them to 8 genes. These genes are expected to be a kidney development and regeneration factor. We hope that it is useful in the future of the various studies.
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