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Ca Load Induces Vascular Smooth Muscle Cell Mineralization Partly Mediated by TRPV

Research Project

Project/Area Number 25461255
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionShowa University

Principal Investigator

MIZOBUCHI MASAHIDE  昭和大学, 医学部, 講師 (90465203)

Co-Investigator(Kenkyū-buntansha) OGATA Hiroaki  昭和大学, 横浜市北部病院内科, 准教授 (30296959)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords血管石灰化 / 血管平滑筋細胞 / カルシウム / TRPVチャネル / Ca / TRPV / アポトーシス
Outline of Final Research Achievements

Although hypercalcemia is a risk factor for vascular calcification, little is known about the involvement of calcium (Ca) in the process of vascular calcification. Human aortic VSMCs were incubated in a low Ca media (LCa), normal Ca media (NCa), and a high Ca media (HCa). VSMC mineralization, and gene expression of markers for osteogenic process and a Ca channel (TRPV-2) was determined. RNA interference for TRPV-2 was performed to study the effect of TRPV2 on the mineralization. VSMC mineralization was induced in a dose dependent manner with the induction of an osteogenic process which was confirmed by the increase in Runx2 mRNA and ALP mRNA expression. We confirmed the up-regulation of TRPV-2 in the HCa. When TRPV-2 mRNA was knocked-down, the mineralization was significantly suppressed in HCa.
These results suggest that Ca load induced VSMC mineralization which is partly mediated by TRPV2. Thus Ca load is a considerable factor for the process of VSMC mineralization.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2014

All Presentation (1 results)

  • [Presentation] Ca Load Induces Vascular Smooth Muscle Cell Mineralization Partly Mediated by TRPV2014

    • Author(s)
      溝渕正英
    • Organizer
      米国腎臓学会議
    • Place of Presentation
      フィラデルフィア
    • Year and Date
      2014-11-11 – 2014-11-16
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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