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The molecular functions of Optineurin in the ALS pathogenesis

Research Project

Project/Area Number 25461280
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurology
Research InstitutionHiroshima University

Principal Investigator

NAGANO YOSHITO  広島大学, 医歯薬保健学研究院(医), 助教 (50397973)

Co-Investigator(Kenkyū-buntansha) 高橋 哲也  広島大学, 病院(医), 講師 (00435942)
松本 昌泰  広島大学, 医歯薬保健学研究院(医), 教授 (20192346)
Co-Investigator(Renkei-kenkyūsha) KAWAKAMI Hideshi  広島大学, 原爆放射線医科学研究所, 教授 (70253060)
Research Collaborator YAO Tso-Pang  Duke University, Professor
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords筋萎縮性側索硬化症 / 筋萎縮 / Optineurin / C2C12 / autophagy / NF-kB / TRAF6 / NFkB / 筋委縮性側索硬化症
Outline of Final Research Achievements

To clarify the molecular functions of Optineurin(OPTN) which is one of ALS-causative gene, in muscle homeostasis, we used murine myoblast C2C12 cells. After differentiation to myotube, Tweak treatment could induce myotube atrophy. In this muscle atrophy model cells, OPTN expression levels of protein and mRNA were not changed during atrophy process. Furthermore OPTN knockdown by siRNA did not affect myotube atrophy induced by Tweak. On the other hand,OPTN may be involved in muscle differentiation process. OPTN protein expression was decreased gradually in that process,and OPTN knockdown promoted myogenic differentiation by regulating myogenic regulatory factor,such as Myf5. In summary, OPTN would not be involved in Tweak-induced muscle atrophy, however it may function as a negative regulator of muscle differentiation.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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