Establishment of measurement and clinical indication of anti pendrin antibody

• Project/Area Number: 25461387
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Tottori University
• Principal Investigator: YOSHIDA Akio 鳥取大学, 医学(系)研究科(研究院), 特任教授 (90158428)
• Co-Investigator(Kenkyū-buntansha): HISATOME Ichiro 鳥取大学, 医学系研究科, 教授 (60211504) ; 鈴木 幸一 帝京大学, 医療技術学部, 教授 (20206478)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Koichi 帝京大学, 医療技術部, 教授 (20206478)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 甲状腺 / ヨード / ペンドリン / イオン輸送体 / 陰イオン交換体 / 橋本病 / バセドウ病 / ELISA / ヨード輸送体 / pendrin / Immunopercipitation / Graves disease / Hashimoto thyroiditis / painless thyroiditis / subacue thyroiditis / anti-pendrin antibody / Graves' disease / subacute thyroiditis

Outline of Final Research Achievements

Autoimmune thyroid diseases are diagnosed by existence of autoーantibody against　thyroglobulin and/or thyro-peroxidase in the patients’ sera. Pendrin is　thyroid specific protein existｓ in the apical membrane of thyroid cells that transportｓ iodide. We have revealed that all of the patients with Hashimoto disease have anti-pendrin antibody in the sera using western blot analysis. We tried to establish ELISA for this antibody for the advance of diagnosis of autoimmune thyroid disease. We have obtained several results that will contribute the establishment of ELISA in the near future.

Research Products

• [Journal Article] Instability of KCNE1-D85N that Causes Long QT Syndrome: Stabilization by Verapamil (2014)
  • Author(s): Sakata S, Kurata Y, Li P, Notsu T, Morikawa K, Miake J, Higaki K, Yamamoto Y, Yoshida A, Shirayoshi Y, Yamamoto K, Horie M, Ninomiya H, Kanzaki S, Hisatome I.
  • Journal Title: Pacing Clinical Electrophysiology Volume: Epub ahead of print Issue: 7 Pages: 1-11
  • DOI: 10.1111/pace.12360
  • Peer Reviewed / Open Access
• [Journal Article] Effects of azelnidipine on uric acid metabolism in patients with essential hypertension. (2014)
  • Author(s): Igawa O, Ogino K, Kato M, Yoshida A, Ninomiya H, Cheng J, Moriwaki Y, Yamamoto K, Hisatome I.
  • Journal Title: Clin Exp Hypertens. Volume: 16
  • Peer Reviewed
• [Journal Article] Hsp90 prevents interaction between CHIP and HERG proteins to facilitate maturation of wild-type and mutant HERG proteins (2013)
  • Author(s): Iwai C, P Li,
  • Journal Title: Cardiovasc Res Volume: ３ Issue: 3 Pages: 520-528
  • DOI: 10.1093/cvr/cvt200
  • Peer Reviewed
• [Journal Article] AMP deaminase 3 plays a critical role in remote reperfusion lung injury. (2013)
  • Author(s): Li P, Ogino K, Hoshikawa Y, Morisaki H, Toyama K, Morisaki T, Morikawa K, Ninomiya H, Yoshida A, Hashimoto K, Shirayoshi Y, Hisatome I.
  • Journal Title: Biochem Biophys Res Commun. Volume: 26 Pages: 131-136
  • Peer Reviewed
• [Journal Article] Transplantation of freshly isolated adipose tissue-derived regenerative cells enhances angiogenesis in a murine model of hind limb ischemia. (2013)
  • Author(s): Harada Y, Yamamoto Y, Tsujimoto S, Matsugami H, Yoshida A, Hisatome I.
  • Journal Title: Biomed Res. Volume: 34 Pages: 23-29
  • NAID: 130004470831
  • Peer Reviewed