Regulation of hematopoietic stem cell function by calorie restriction

• Project/Area Number: 25461411
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Kanazawa University
• Principal Investigator: Tadokoro Yuko 金沢大学, がん進展制御研究所, 助教 (00447343)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 幹細胞 / カロリー制限

Outline of Final Research Achievements

Recent studies have indicated that calorie restriction affects the regulation of tissue specific stem cells. Thus, calorie control has been thought as a novel regulatory machinery of stem cell regulation. In this study, I have investigated how calorie restriction influences regulation of hematopoietic stem cell system. I found that calorie restriction phenotypically increased the number of hematopoietic stem cells, while there is no advantage in stem cell function.

Research Products

• [Journal Article] Strong therapeutic potential of γ-secretase inhibitor MRK003 for CD44-high and CD133-low glioblastoma initiating cells. (2015)
  • Author(s): Tanaka S, Nakada M, Yamada D, Nakano I, Todo T, Ino Y, Hoshii T, Tadokoro Y, Ohta K, Ali MA, Hayashi Y, Hamada J, Hirao A.
  • Journal Title: J Neurooncol. Volume: 121 Issue: 2 Pages: 239-50
  • DOI: 10.1007/s11060-014-1630-z
  • Peer Reviewed / Open Access
• [Journal Article] Association of a murine leukaemia stem cell gene signature based on nucleostemin promoter activity with prognosis of acute myeloid leukaemia in patients. (2014)
  • Author(s): Ali MA, Naka K, Yoshida A, Fuse K, Kasada A, Hoshii T, Tadokoro Y, Ueno M, Ohta K, Kobayashi M, Takahashi C, Hirao A.
  • Journal Title: Biochem Biophys Res Commun. Volume: 450 Issue: 1 Pages: 837-843
  • DOI: 10.1016/j.bbrc.2014.06.066
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Loss of Tsc1 accelerates malignant gliomagenesis when combined with oncogenic signals. (2014)
  • Author(s): Yamada D, Hoshii T, Tanaka S, Hegazy AM, Kobayashi M, Tadokoro Y, Ohta K, Ueno M, Ali MAE, Hirao A.
  • Journal Title: J Biochem. Volume: 155 Issue: 4 Pages: 227-33
  • DOI: 10.1093/jb/mvt112
  • Peer Reviewed
• [Journal Article] Abundant nucleostemin expression supports the undifferentiated properties of germ cell tumors (2013)
  • Author(s): Uema, N., Ooshio, T., Harada, K., Naito, M., Naka, K., Hoshii, T., Tadokoro, Y., Ohta, K., Ali, M.A., Katano, M., Soga, T., Nakanuma, Y., Okuda, A., Hirao, A.
  • Journal Title: Am. J. Pathol. Volume: 183 Issue: 2 Pages: 592-603
  • DOI: 10.1016/j.ajpath.2013.04.018
  • Peer Reviewed
• [Journal Article] Loss of mTOR complex 1 induces developmental blockage in early T-lymphopoiesis and eradicates T-cell acute lymphoblastic leukemia cells. (2013)
  • Author(s): Hoshii T, Kasada A, Hatakeyama T, Ohtani M, Tadokoro Y, Naka K, Ikenoue T, Ikawa T, Kawamoto H, Fehling HJ, Araki K, Yamamura K, Matsuda S, Hirao A
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111 Issue: 10 Pages: 3805-3810
  • DOI: 10.1073/pnas.1320265111
  • Peer Reviewed
• [Presentation] Spred1 regulates the self-renewal activity of hematopoietic stem cells (2015)
  • Author(s): Yuko Tadokoro, Akihiko Yoshimura, Hiromitsu Nakauchi, Atsushi Hirao
  • Organizer: 第77回 日本血液学会学術集会
  • Place of Presentation: 石川県立音楽堂（石川県）
  • Year and Date: 2015-10-16
• [Presentation] Role of Spred1 in the regulation of self-renewal and aging of hematopoietic stem cells (2015)
  • Author(s): Yuko Tadokoro, Akihiko Yoshimura, Hiromitsu Nakauchi, Atsushi Hirao
  • Organizer: ISEH 44rd Annual Scientific Meeting
  • Place of Presentation: 国立京都国際会館（京都府）
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Presentation] Spred1 regulates the self-renewal activity in hematopoietic stem cells (2015)
  • Author(s): Yuko Tadokoro, Akihiko Yoshimura, Hiromitsu Nakauchi, Atsushi Hirao
  • Organizer: The 10th International Symposium of the Institute Network
  • Place of Presentation: 北海道大学 遺伝子病制御研究所（北海道）
  • Year and Date: 2015-07-23
  • Int'l Joint Research
• [Presentation] Quality control of undifferentiated status in hematopoietic stem cells during cell division by Spred1 (2015)
  • Author(s): Yuko Tadokoro, Akihiko Yoshimura, Hiromitsu Nakauchi, Atsushi Hirao
  • Organizer: The 13th Stem Cell Research Symposium
  • Place of Presentation: 東京大学 伊藤国際学術研究センター（東京）
  • Year and Date: 2015-05-29
• [Presentation] Control of self-renewal activity of hematopoietic stem cells by Spred1 (2015)
  • Author(s): Yuko Tadokoro, Koji Eto, Hideo Ema, Satoshi Yamazaki, Akihiko Yoshimura, Hiromitsu Nakauchi, Atsushi Hirao
  • Organizer: 日本癌学会シンポジウム/共同利用・共同研究拠点シンポジウム
  • Place of Presentation: 石川県立音楽堂交流ホール（石川県）
  • Year and Date: 2015-01-21 – 2015-01-22
• [Presentation] Control of self-renewal activity of hematopoietic stem cells by Spred1 (2014)
  • Author(s): Yuko Tadokoro, Koji Eto, Hideo Ema, Satoshi Yamazaki, Akihiko Yoshimura, Hiromitsu Nakauchi, Atsushi Hirao
  • Organizer: ISEH 43rd Annual Scientific Meeting
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] Inhibitory effect of Spred1 on self-renewal activity mediated by cell-cell interaction determines size of hematopoietic stem cell pool (2014)
  • Author(s): Yuko Tadokoro, Takayuki Hoshii, Kazuhito Naka, Koji Eto, Hideo Ema, Satoshi Yamazaki, Akihiko Yoshimura, Hiromitsu Akauchi, Atsushi Hirao
  • Organizer: 12th Stem Cell Research Symposium
  • Place of Presentation: 九州大学百年講堂（福岡県）
  • Year and Date: 2014-05-30 – 2014-05-31
• [Presentation] Spred-1 regulates the competitive advantage of hematopoietic stem cells in the bone marrow microenvironment (2014)
  • Author(s): Yuko Tadokoro, Atsushi Hirao, Takayuki Hoshii, Kazuhito Naka, Koji Eto, Hideo Ema, Satoshi Yamazaki, Akihiko Yoshimura, Hiromitsu Nakauchi
  • Organizer: International Symposium on Tumor Biology in Kanazawa & Symposium on Drug Discovery in Academics
  • Place of Presentation: 金沢エクセルホテル東急（石川県）
• [Remarks] 遺伝子・染色体構築研究分野ホームページ
  • URL: http://cri-mol-gen.w3.kanazawa-u.ac.jp/