| Project/Area Number |
25461419
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
Mizuki Masao 大阪大学, 医学部附属病院, 准教授 (80283761)
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| Co-Investigator(Kenkyū-buntansha) |
KANAKURA Yuzuru 大阪大学, 医学系研究科, 教授 (20177489)
SHIBAYAMA Hirohiko 大阪大学, 医学系研究科, 講師 (60346202)
FUJITA Jiro 大阪大学, 医学系研究科, 助教 (90608720)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 樹状細胞 / 白血病関連遺伝子 / cDC / pDC / 組織球系腫瘍 / 白血病関連遺伝子変異 / DC / 白血病 / ｃDC / ｐDC |
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| Outline of Final Research Achievements |
We have established the dendritic cell (DC) differentiation system from murine hematopoietic stem cell fraction. We have shown that type I leukemia-related gene abnormalities except for FLT3 and ras, blocked DC differentiation and preferentially induced the differentiation of conventional DC (cDC) compared to plasmacytoid DC. In patients with TEL-PDGFRbeta positive chronic myelomonocytic leukemia, cDC subset was found to be derived from leukemic clone. In patients with acute myelogeneous leukemia showing spontaneous DC differentiation, there was a CD8+T cell subset, which reacted with leukemia-specific antigen, WT1, suggesting that leukemia-derived DC could present leukemia-specific antigens. Finally, origin-unknown soft tissue tumor was found to be histiocytic lineage, by whole exome sequensing which showed MAP2K1 mutation.
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