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Functional analysis of the protein phosphatase expressed in dendritic cells

Research Project

Project/Area Number 25461468
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Collagenous pathology/Allergology
Research InstitutionGunma University

Principal Investigator

KANEKO YORIAKI  群馬大学, 医学部附属病院, 講師 (00334095)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords自己免疫疾患 / ホスファターゼ / 自己免疫 / 樹状細胞 / Shp-1 / SHP-1
Outline of Final Research Achievements

We generated a conditional knockout mouse (Shp-1 cKO) in which Shp-1, an intracytoplasmic tyrosine phosphatase, is specifically depleted in dendritic cells (DCs). Shp-1 cKO developed autoimmune nephritis with aging, which was characterized by massive infiltration of macrophages and T cells. The cells prepared from the kidneys of Shp-1 cKO were able to produce large amounts of inflammatory cytokines, therefore we conclude Shp-1 in DCs acts as a negative regulator for the development of autoimmune nephritis.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Dendritic cell SIRPα regulates homeostasis of dendritic cells in lymphoid organs2015

    • Author(s)
      Washio K, Kotani T, Saito Y, Respatika D, Murata Y, Kaneko Y, Okazawa H, Ohnishi H, Fukunaga A, Nishigori C, Matozaki T.
    • Journal Title

      Genes Cells

      Volume: - Issue: 6 Pages: 00-00

    • DOI

      10.1111/gtc.12238

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] SIRPα signaling regulates podocyte structure and function2013

    • Author(s)
      Takahashi S, Tomioka M, Hiromura K, Sakairi T, Hamatani H, Watanabe M, Ikeuchi H, Kaneko Y, Maeshima A, Aoki T, Ohnishi H, Matozaki T, Nojima Y
    • Journal Title

      Am J Physiol Renal Physiol

      Volume: 305 Issue: 6 Pages: 861-70

    • DOI

      10.1152/ajprenal.00597.2012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp-1 induces autoimmune nephritis characterized by infiltration of dendritic cell and Th1 cell2015

    • Author(s)
      WATANABE Mitsuharu, KANEKO Yoriaki, HIROMURA Keiju, OHISHI Yuko, KINOSHITA Masato, SAITO Yasuyuki, OHNISHI Hiroshi, MATOZAKI Takashi, NOJIMA Yoshihisa
    • Organizer
      The 44th annual meeting of the Japanese Society of Immunology
    • Place of Presentation
      Sapporo
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 induces enhanced production of inflammatory cytokines by toll-like receptor-mediated stimulation2015

    • Author(s)
      OHISHI Yuko, KANEKO Yoriaki, WATANABE Mitsuharu, KINOSHITA Masato, HIROMURA Keiju, SAITO Yasuyuki, OHNISHI Hiroshi, MATOZAKI Takashi, NOJIMA Yoshihisa
    • Organizer
      The 44th annual meeting of the Japanese Society of Immunology
    • Place of Presentation
      Sapporo
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dendritic Cell-Specific Shp-1-Knockout Mice Spontaneously Develop Unique Glomerulo- and Tubulointerstitial Nephritis2015

    • Author(s)
      Mitsuharu Watanabe, Keiju Hiromura, Yoriaki Kaneko, Masato Kinoshita, Yuko Ohishi, Toru Sakairi, Hidekazu Ikeuchi, Akito Maeshima, Hiroshi Ohnishi, Takashi Matozaki, Yoshihisa Nojima
    • Organizer
      Kidney Week 2015, American Society of Nephrology
    • Place of Presentation
      San Diego, CA
    • Year and Date
      2015-11-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Essential role of SIRPα on dendritic cells in organizing and homeostasis of the spleen2014

    • Author(s)
      Washio Ken, Kotani Takenori, Respatika Datu, Murata Yoji, Saito Yasuyuki, Kaneko Yoriaki, Okazawa Hideki, Ohnishi Hiroshi, Fukunaga Atsushi, Nishigori Chikako, Matozaki Takashi
    • Organizer
      The 43rd annual meeting of the Japanese society of immunology
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-12-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Immunological analysis of autoimmune nephritis observed in DC-specific Shp1 conditional knockout mice.2014

    • Author(s)
      Watanabe Mitsuharu, Kaneko Yoriaki, Hiromura Keiju, Ohishi Yuko, Kinoshita Masato, Ohnishi Hiroshi, Matozaki Takashi, Nojima Yoshihisa
    • Organizer
      The 43rd annual meeting of the Japanese society of immunology
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-12-04
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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