| Project/Area Number |
25461477
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI ATSUSHI 長崎大学, 医歯薬学総合研究科(医学系), 教授 (90325639)
NAKAMURA TATSUFUMI 長崎大学, 医歯薬学総合研究科(医学系), 准教授 (00198219)
HORAI YOSHIRO 長崎大学, 病院(医学系), 医員 (30646782)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | シェーグレン症候群 / HTLV-I / 膠原病 / ICAM-１ / IP-10 / RANTES / アポトーシス |
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| Outline of Final Research Achievements |
To explore whether HTLV-I infects salivary gland epithelial cells (SGECs) of Sjogren’s syndrome (SS).We determined the inflammation-related molecules profiles after the co-culture of SGECs with HCT-5. The apoptosis-related molecules profile was determined by antibody dot-blot array and IF. We investigated the presence of HTLV-I-related molecules by IF and in situ PCR. The apoptosis of SGECs was evaluated by TUNEL staining.7.8% of the SGECs were positive for HTLV-I-related proteins afterco-culture. HTLV-I proviral DNA was detected by in situ PCR. Co-cultured supernatantsshowed time-dependent increases of sICAM-1, RANTES, and IP-10/CXCL10. The expressions of pro-apoptotic molecules and anti-apoptotic molecules were also increased in the SGECs. Apoptosis of SGECs was not detected after co-culture.HTLV-I could infect SGECs and alter their cellular functions. These findings contribute to the development of HTLV-I-associated SS.
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