| Project/Area Number |
25461490
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
Fujii Ryoji 聖マリアンナ医科大学, 医学研究科, 講師 (10333535)
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| Co-Investigator(Renkei-kenkyūsha) |
YUDO Kazuo 聖マリアンナ医科大学, 医学研究科, 教授 (60272928)
KOMATSU Rie 聖マリアンナ医科大学, 医学研究科, 研究技術員 (80517475)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 関節リウマチ / 滑膜 / 細胞増殖 / 炎症 / 滑膜炎 / リウマチ |
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| Outline of Final Research Achievements |
SPACIA1 is a gene associated with abnormal synovial proliferation in rheumatoid arthritis (RA). We previously showed that SPACIA1 transgenic mice exhibited early onset and rapid progression of collagen-induced arthritis (CIA). In the present study, we showed SPACIA1 could be involved in expression of CDK6 gene, a G1 phase-cell cycle factor, via mRNA stability. We also confirmed that TNF-alpha regulates CDK6 expression on transcriptional level in RA-synoviocytes. Unfortunately, even SPACIA1-deficient mice were investigated onset of CIA, therefore we focused CDK6, not SPACIA1, to assess the potential as a druggable target of RA. CDK6 knockdown by its siRNA inhibited the proliferation of RA-synoviocytes. Moreover, treatment of CDK6 inhibitor to mice almost completely prevented development of CIA.
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