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Development of rapid diagnostic system for causative organisms of pneumonia and drug resistance using real-time PCR and its application to primary care settings

Research Project

Project/Area Number 25461517
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Infectious disease medicine
Research InstitutionNagasaki University

Principal Investigator

KUBO Toru  長崎大学, 熱帯医学研究所, 客員研究員 (50444873)

Co-Investigator(Kenkyū-buntansha) MORIUCHI Hiroyuki  長崎大学, 医歯薬学総合研究科(医学系), 教授 (90315234)
NISHIMURA Hidekazu  独立行政法人国立病院機構仙台医療センター臨床研究部, 臨床研究部ウイルスセンター, 臨床研究部ウイルスセンター長 (50172698)
Co-Investigator(Renkei-kenkyūsha) MORITA Kouichi  長崎大学, 熱帯医学研究所, 教授 (40182240)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords感染症診断学 / 肺炎 / リアルタイムPCR
Outline of Final Research Achievements

Pneumonia is the No. 3 cause of death in Japan now. The number of patients of pneumonia is still increasing. Rapid detection of causative organism and drug resistance is important in pneumonia therapy. We have developed easy, rapid diagnostic system of pneumonia using real-time PCR system, which can be utilized in primary care clinics. This low-cost, rapid definitive diagnostic system may contribute to establish more effective control model of pneumonia including TB in our aging society even in rural settings.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (7 results)

  • [Journal Article] A novel method for rapid detection of Streptococcus pneumoniae antigens in blood.2016

    • Author(s)
      Fukushima K, Kubo T, Ehara N, Nakano R, Matsutake T, Ishimatu Y, Tanaka Y, Akamatsu S, Izumikawa K, Kohno S.
    • Journal Title

      J Infect Chemother.

      Volume: 22 Issue: 3 Pages: 143-148

    • DOI

      10.1016/j.jiac.2015.12.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Elevated levels of full-length and thrombin-cleaved osteopontin during acute dengue virus infection are associated with coagulation abnormalities.2014

    • Author(s)
      Chagan-Yasutan H, Lacuesta TL, Ndhlovu LC, Oguma S, Leano PS, Telan EF, Kubo T, Morita K, Uede T, Dimaano EM, Hattori T
    • Journal Title

      Thrombosis research

      Volume: 139 Issue: 2 Pages: 449-454

    • DOI

      10.1016/j.thromres.2014.05.003

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Survey of causative agents for acute respiratory infections among patients in Khartoum-State, Sudan, 2010-20112013

    • Author(s)
      Enan, K. A., Nabeshima, T., Kubo, T., Buerano, C. C., El Hussein, A. R., Elkhidir, I. M., Khalil, E. A., Morita, K
    • Journal Title

      Virology Journal

      Volume: 10 Issue: 1 Pages: 312-321

    • DOI

      10.1186/1743-422x-10-312

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 遺伝子検査法による薬剤耐性マイコプラズマの検出に関する研究2016

    • Author(s)
      久保亨、松竹豊司、江原尚美、中野令伊司、金子裕子、小野靖彦、井上幸子、河野茂、福島喜代康
    • Organizer
      日本マイコプラズマ学会第43回学術集会
    • Place of Presentation
      長崎県医師会館(長崎)
    • Year and Date
      2016-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 長崎県央部で分離された薬剤耐性結核菌のリアルタイムPCR法を用いた分子疫学解析2015

    • Author(s)
      久保亨、松竹豊司、江原尚美、森田公一、河野茂、福島喜代康
    • Organizer
      第89回日本感染症学会総会学術集会
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2015-04-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遺伝子シーケンシングを用いた薬剤耐性結核の迅速判定法の日常臨床への応用に関する研究2015

    • Author(s)
      久保 亨、松竹豊司、江原尚美、中野 令伊司、金子 裕子、森田公一、河野 茂、福島喜代康
    • Organizer
      第90回日本結核病学総会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2015-03-27 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] リアルタイムPCR法を用いた結核菌のVNTR解析の日常臨床への応用に関する研究2015

    • Author(s)
      久保 亨、松竹豊司、江原尚美、中野 令伊司、金子 裕子、森田公一、河野 茂、福島喜代康
    • Organizer
      第90回日本結核病学総会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2015-03-27 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 分子生物学的手法を用いたインフルエンザの院内感染制御に関する研究2014

    • Author(s)
      久保 亨、松竹豊司、江原尚美、森田公一、河野 茂、福島喜代康
    • Organizer
      第88回日本感染症学会学術講演会
    • Place of Presentation
      ヒルトン福岡シーホーク(福岡県福岡市)
    • Year and Date
      2014-06-18 – 2014-06-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 遺伝子シーケンシング法を用いた結核菌の薬剤耐性の迅速判定の日常診療への応用に関する研究2014

    • Author(s)
      久保 亨、松竹豊司、江原尚美、森田公一、河野 茂、福島喜代康
    • Organizer
      第88回日本感染症学会学術講演会
    • Place of Presentation
      ヒルトン福岡シーホーク(福岡県福岡市)
    • Year and Date
      2014-06-18 – 2014-06-20
    • Related Report
      2014 Research-status Report
  • [Presentation] リアルタイムPCR法を用いた結核の分子診断法の日常診療への応用に関する研究2013

    • Author(s)
      久保 亨、松竹豊司、江原尚美、森田公一、河野 茂、福島喜代康
    • Organizer
      第87回日本感染症学会総会学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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