Exploring causal genes in susceptibility loci associated with atopic dermatitis and common genetic factors among allergic diseases

• Project/Area Number: 25461612
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Hirota Tomomitsu 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 研究員 (50435674)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: アレルギー / アトピー性皮膚炎 / GWAS / SNP / 関連遺伝子 / 疾患関連解析 / アレルギー疾患 / 遺伝子多型

Outline of Final Research Achievements

For a better understanding of the pathological mechanisms underlying AD, we sought to identify the potential causal gene in an AD-associated locus (3p21.33) identified by AD GWAS. We investigated eQTL databases, and conducted analyses for histone modification (methylation and acetylation) and three-dimensional chromatin structure in this region. We confirmed that DNA sequence in an intron of the GLB1 gene at the locus is presumed to regulate CCR4 gene expression. These findings suggest that CCR4 is a plausible candidate gene in the region.

Research Products

• [Presentation] アレルギー疾患のGWAS (2015)
  • Author(s): 広田朝光
  • Organizer: 第64回 日本アレルギー学会学術大会
  • Place of Presentation: グランドプリンスホテル新高輪 国際館パミール, 港区, 東京
  • Year and Date: 2015-05-27
  • Invited
• [Presentation] A variant of Interleukine-13 (IL13) gene is associated with food allergy in the Japanese population (2014)
  • Author(s): Tomomitsu Hirota
  • Organizer: The 30th Symposium of the Collegium Internationale Allergologicum
  • Place of Presentation: Steigenberger Hotel, Petersberg, Germany
  • Year and Date: 2014-09-13 – 2014-09-18
• [Presentation] Polymorphism of Interleukine-13 (IL13) are associated with susceptibility to food allergy in the Japanese population (2014)
  • Author(s): Tomomitsu Hirota
  • Organizer: European Academy of Allergy and Clinical Immunology Congress 2014
  • Place of Presentation: Bella Center, Copenhagen, Denmark
  • Year and Date: 2014-06-07 – 2014-06-11
• [Presentation] アレルギー疾患の関連遺伝子探索 (2014)
  • Author(s): 広田朝光
  • Organizer: 日本アレルギー学会
  • Place of Presentation: 国立京都国際会館、京都、日本
  • Year and Date: 2014-05-09 – 2014-05-11
• [Presentation] Genome-wide association study (GWAS) of atopic dermatitis in the Japanese population (2013)
  • Author(s): Tomomitsu Hirota
  • Organizer: KAPARD-KAAACI West Pacific Allergy Symposium Joint International Congress
  • Place of Presentation: Seoul, Korea
  • Invited
• [Presentation] Genome-wide association study (GWAS) of atopic dermatitis in the Japanese population (2013)
  • Author(s): Tomomitsu Hirota
  • Organizer: Asia Pacific Congress of Allergy, Asthma and Clinical Immunology 2013
  • Place of Presentation: Taipei, Taiwan
  • Invited
• [Presentation] A polymorphism of Interleukine-13 (IL13) is associated with susceptibility to food allergy in the Japanese population. (2013)
  • Author(s): Tomomitsu Hirota
  • Organizer: Annual Meeting of the American Society of Human Genetics
  • Place of Presentation: Boston, USA