The effect of CD28 signal in podocyte injury

• Project/Area Number: 25461624
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: University of Miyazaki
• Principal Investigator: konomoto takao 宮崎大学, 医学部, 助教 (80315366)
• Co-Investigator(Kenkyū-buntansha): FUKUDA Akihiro 宮崎大学, 医学部, 医員 (30628889) ; FUJIMOTO Shouichi 宮崎大学, 医学部, 教授 (80173467)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ポドサイト / CD８０ / CD２８ / アポトーシス / 酸化ストレス / CD80 / CD28

Outline of Final Research Achievements

We investigate that effect of CD80-CD28 signal in podocyte injury. Both of CD80 and CD28 expression were upregulated by podocyte injury stimuli such as angiotensin 2 and polyI;C. Immunoprecipitation analysis showed that CD80 combined with CD28. Activating CD28 signal by anti-CD28 antibody induced decreasing of actin stress fiber in podocyte. However we could not fined the molecules, which were activated by CD28 signal.