Anomalies of the 4-6 pharyngeal arch arteries and the lung development in Foxc2 deficient mouse embryos
| Project/Area Number |
25461632
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
清水 一彦 東京女子医科大学, 医学部, 助教 (90385394)
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| Co-Investigator(Renkei-kenkyūsha) |
EZAKI Taichi 東京女子医科大学, 医学部, 教授 (10128259)
MORIKAWA Shun-ichi 東京女子医科大学, 医学部, 講師 (70339000)
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| Research Collaborator |
TSUJI Mayoko 東京女子医科大学, 医学部, 大学院生
ENERBÄCK Sven イェーテボリ大学, 医学部, 教授
HEGLIND Mikael イェーテボリ大学, 医学部, 研究員
KUME Tsutomu ノースウェスタン大学, 医学部, 教授
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Foxc2 遺伝子 / 先天性心疾患 / 心肺前駆細胞 / 鰓弓動脈 / 肺発生 / 肺胞上皮前駆細胞 / 循環器発生 / Foxc2遺伝子 / 肺微小循環形成 |
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| Outline of Final Research Achievements |
Foxc2 deficient mouse (Foxc2-/-) embryos show abnormal morphology of pharyngeal arch arteries (PAAs) during organogenesis. The 5th PAA-like structure, which is not appeared in wild type (WT) embryos, is often detected in Foxc2-/- embryos. We examined the distribution of PAA endothelia in pharyngeal arches for checking PAAs pattern, and also analyzed canonical gene expression during lung development from embryonic day (E) 10.5 to 18.5 in both WT and Foxc2-/-. Immunochemical stain with CD31, a marker of vascular endothelia, revealed scattered CD31+ cells between the 4th and the 6th PAA, such as extra-PAA-like structures in pharyngeal arches. Results of real time PCR showed Lef1 gene level was decreased in Foxc2-/- at E11.5. In WT embryos, Lef1 protein was expressed in the distal lung mesenchyme adjacent to the lung epithelium, however, the expression was downregulated in Foxc2-/- at E11.5.
Our data indicates that Foxc2 gene might be involved in the lung development.
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Report
(5 results)
Research Products
(16 results)
