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Anomalies of the 4-6 pharyngeal arch arteries and the lung development in Foxc2 deficient mouse embryos

Research Project

Project/Area Number 25461632
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatrics
Research InstitutionTokyo Women's Medical University

Principal Investigator

Morishima Masae  東京女子医科大学, 医学部, 助教 (00241068)

Co-Investigator(Kenkyū-buntansha) 清水 一彦  東京女子医科大学, 医学部, 助教 (90385394)
Co-Investigator(Renkei-kenkyūsha) EZAKI Taichi  東京女子医科大学, 医学部, 教授 (10128259)
MORIKAWA Shun-ichi  東京女子医科大学, 医学部, 講師 (70339000)
Research Collaborator TSUJI Mayoko  東京女子医科大学, 医学部, 大学院生
ENERBÄCK Sven  イェーテボリ大学, 医学部, 教授
HEGLIND Mikael  イェーテボリ大学, 医学部, 研究員
KUME Tsutomu  ノースウェスタン大学, 医学部, 教授
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsFoxc2 遺伝子 / 先天性心疾患 / 心肺前駆細胞 / 鰓弓動脈 / 肺発生 / 肺胞上皮前駆細胞 / 循環器発生 / Foxc2遺伝子 / 肺微小循環形成
Outline of Final Research Achievements

Foxc2 deficient mouse (Foxc2-/-) embryos show abnormal morphology of pharyngeal arch arteries (PAAs) during organogenesis. The 5th PAA-like structure, which is not appeared in wild type (WT) embryos, is often detected in Foxc2-/- embryos. We examined the distribution of PAA endothelia in pharyngeal arches for checking PAAs pattern, and also analyzed canonical gene expression during lung development from embryonic day (E) 10.5 to 18.5 in both WT and Foxc2-/-. Immunochemical stain with CD31, a marker of vascular endothelia, revealed scattered CD31+ cells between the 4th and the 6th PAA, such as extra-PAA-like structures in pharyngeal arches. Results of real time PCR showed Lef1 gene level was decreased in Foxc2-/- at E11.5. In WT embryos, Lef1 protein was expressed in the distal lung mesenchyme adjacent to the lung epithelium, however, the expression was downregulated in Foxc2-/- at E11.5.
Our data indicates that Foxc2 gene might be involved in the lung development.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] University of Gothenburg(Sweden)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Foxc2 influences alveolar epithelial cell differentiation during lung development2017

    • Author(s)
      Mayoko Tsuji, Masae Morishima, Kazuhiko Shimizu, Shunichi Morikawa, Mikael Heglind, Sven Enerback, Taichi Ezaki, Jun Tamaoki
    • Journal Title

      Development Growth and Differentiation

      Volume: 59 Issue: 6 Pages: 501-514

    • DOI

      10.1111/dgd.12368

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] モデルマウスの円錐動脈幹と大血管の形態異常ー心大血管発生と表現型ー2017

    • Author(s)
      森島正恵
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎大学坂本キャンパス(長崎県・長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Foxc2遺伝子の肺発生過程における発現と血管新生に及ぼす影響2017

    • Author(s)
      辻真世子、森島正恵、清水一彦、近藤光子、別役智子、久米努、玉置淳、江崎太一
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎大学坂本キャンパス(長崎県・長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Role Of FOXC2 In Differentiation Of Alveolar Type1 Epithelial Cells2016

    • Author(s)
      Mayoko Tsuji, Masae Morishima, Kazuhiko Shimizu, Mitsuko Kondo, Tsutomu Kume, Jun Tamaoki, and Taichi Ezaki
    • Organizer
      American Thoracic Society Conference 2016
    • Place of Presentation
      San Francisco Moscone Center, CA, USA
    • Year and Date
      2016-05-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Foxc2遺伝子が肺発生過程において間葉細胞に及ぼす影響2016

    • Author(s)
      辻真世子、森島正恵、清水一彦、近藤光子、別役智子、久米努、玉置淳、江崎太一
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレット福島(福島・郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] APC-Min/+マウス小腸の腺腫形成領域におけるICCの形態的変化2016

    • Author(s)
      宮本(菊田)幸子、北原秀治、森島正恵、江崎太一
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレット福島(福島・郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 肺発生におけるFoxc2遺伝子の役割2015

    • Author(s)
      辻真世子、森島正恵、清水一彦、近藤光子、玉置淳、久米努、江﨑太一
    • Organizer
      第55回呼吸器学会総会
    • Place of Presentation
      東京国際フォーラム{東京都・千代田区}
    • Year and Date
      2015-04-17 – 2015-04-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Foxc2 欠失マウスにおける心血管奇形の形態2015

    • Author(s)
      森島正恵、辻真世子、清水一彦、Mikael Heglind、Sven, Enerbäck、久米努、中西敏雄、江崎太一
    • Organizer
      第120回日本解剖学会総会・全国学術集会
    • Place of Presentation
      神戸国際会議場・展示場{兵庫県・神戸市)
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Foxc2 ノックアウトマウスにおける胎仔肺組織学的検討2015

    • Author(s)
      辻真世子、森島正恵、清水一彦、近藤光子、玉置淳、久米努、江﨑太一
    • Organizer
      第120回日本解剖学会総会・全国学術集会
    • Place of Presentation
      神戸国際会議場・展示場{兵庫県・神戸市)
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Foxc2 ノックアウトマウスにおける肺成熟過程の組織学的解析2014

    • Author(s)
      辻真世子、森島正恵、清水一彦、北原秀治、玉置淳、久米努、江崎太一
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      自治医科大学キャンパス、栃木
    • Related Report
      2013 Research-status Report
  • [Presentation] VASCULAR DEVELOPMENT IN THE LUNG OF Foxc2 KNOCKOUT MICE2014

    • Author(s)
      Mayoko Tsuji, Masae Morishima, Kazuhiko Shimizu, Mikael Heglind, Sven Enerbäck, Tsutomu Kume, Jun Tamaoki, Taichi Ezaki
    • Organizer
      Weinstein Cardiovascular Development Conference
    • Place of Presentation
      スペイン、マドリード
    • Related Report
      2013 Research-status Report
  • [Presentation] Foxc2 欠損マウス胚仔における鰓弓形態異常の特徴2014

    • Author(s)
      森島正恵、辻真世子、清水一彦、Mikael Heglind、久米努、中西敏雄、Sven Enerbeck、江﨑太一
    • Organizer
      第54回日本先天異常学会学術総会
    • Place of Presentation
      神奈川県、相模原
    • Related Report
      2013 Research-status Report
  • [Presentation] Foxc2 ノックアウトマウスにおける肺成熟過程の組織学的解析2014

    • Author(s)
      辻 真世子, 森島 正恵, 清水 一彦, 玉置 淳, 久米 努, 江﨑 太一
    • Organizer
      第54回日本先天異常学会学術総会
    • Place of Presentation
      神奈川県、相模原
    • Related Report
      2013 Research-status Report
  • [Presentation] Vascular analysis in the lung of Foxc2 deficient mice.2014

    • Author(s)
      Mayoko Tsuji, Masae Morishima, Kazuhiko Shimizu, Tsutomu Kume, Jun Tamaoki, Taichi Ezaki
    • Organizer
      European Respiratory Congress
    • Place of Presentation
      ドイツ
    • Related Report
      2013 Research-status Report
  • [Presentation] Foxc2 ノックアウトマウスにおける肺組織の形態学的解析2013

    • Author(s)
      森島正恵、清水一彦、中西秀彦、北原秀治、Mikael Heglind, Sven Enerback, 久米努、江崎太一
    • Organizer
      第53回日本先天異常学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター、大阪
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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