| Project/Area Number |
25461957
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
Ishitani Akiko 奈良県立医科大学, 医学部, 非常勤講師 (40112544)
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| Co-Investigator(Kenkyū-buntansha) |
王寺 典子 (下嶋典子 / 下嶋 典子 / 王寺 典子(下嶋典子)) 奈良県立医科大学, 医学部, 助教 (30398432)
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| Co-Investigator(Renkei-kenkyūsha) |
ICHINOHE TATSUO 広島大学, 医学系研究科, 教授 (80314219)
UEMOTO SHINJI 京都大学, 医学系研究科, 教授 (40252449)
TAKAHARA SHIRO 大阪大学, 医学系研究科, 教授 (70179547)
MORII TAKESHI 奈良県立医科大学, 医学部, 講師 (70264851)
YOSHIDA KATSUNORI 奈良県立医科大学, 医学部, 教授 (50192422)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | HLA-G / 移植片生着 / 腎移植 / 肝移植 / 造血幹細胞移植 / 移植片 / 生着 / 可溶性HLA-G / HL-G / ELISA / Flowcytometry / 移植 / 遺伝子多型 |
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| Outline of Final Research Achievements |
HLA-G is expressed on placental trophoblasts, the border of mother and fetus, and known to protect fetus from the maternal immune system. It was hypothesized that the immune suppressive function of HLA-G may help engraftment outcome at transplantation. This hypothesis was supported by several papers reporting that HLA-G protein in patient plasma or on the graft were associated with the graft survival.
In this study, HLA-G expression on the peripheral mononuclear cells and in the plasma of patients before and after transplantation were tested. We developed an ELISA to detect very low concentration of HLA-G protein in plasma. Using the method, 167samples from kidney transplantation, 111 from lever transplantation and 52 from hematopoietic stem cell transplantation were tested the HLA-G expression. No detectable levels of HLA-G were found in any samples. From this data, we conclude that the association of HLA-G expression and graft survival in those reports should be reexamined.
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