Mutanome analyses by next generation sequencing and individualized cancer vaccine development in gastric cancer
| Project/Area Number |
25462014
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Wada Ikuo 東京大学, 医学部附属病院, 届出診療医 (80401082)
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| Co-Investigator(Kenkyū-buntansha) |
SETO Yasuyuki 東京大学, 医学部附属病院, 教授 (00260498)
KAKIMI Kazuhiro 東京大学, 医学部附属病院, 特任教授 (80273358)
MATSUSHITA Hirokazu 東京大学, 医学部附属病院, 特任講師 (80597782)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 胃がん / 次世代シーケンス / 遺伝子変異 / MHCクラスI結合予測法 / 固有抗原 / TIL / 個別化がん免疫治療 / 胃癌 / ネオアンチゲン / エクソームシーケンス / ミスセンス変異 / 変異ペプチド / アフィニティ / MHC binding prediction法 |
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| Outline of Final Research Achievements |
We established the method to identify unique antigens from cancer specific mutations using murine gastric cancer model by combining next generation sequencing with MHC class I binding algorism. We obtained cancer tissues and peripheral blood mononuclear cells (PBMCs) from 29 gastric cancer patients. We analyzed the expression of immune checkpoint molecules and the infiltration of regulatory T cells (Tregs) to understand the immune suppressive tumor microenvironment in 10 patients. We successfully cultured tumor infiltrating lymphocytes (TILs) specifically recognizing autologous tumor cells from 3 patients. We created the system predicting candidate unique antigens and obtained promising samples including cancer tissues and TILs, which are a prerequisite for individualized cancer immunotherapy development in gastric cancer patients.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Identification of Individual Cancer-Specific Somatic Mutations for Neoantigen-Based Immunotherapy of Lung Cancer.2016
Author(s)
Karasaki T, Nagayama K, Kawashima M, Hiyama N, Murayama T, Kuwano H, Nitadori J, Anraku M, Sato M, Miyai M, Hosoi A, Matsushita H, Kikugawa S, Matoba R, Ohara O, Kakimi K, Nakajima J
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Journal Title
Journal of Thoracic Oncology
Volume: 11(3)
Issue: 3
Pages: 324-33
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Vaccination With NY-ESO-1 Overlapping Peptides Mixed With Picibanil OK-432 and Montanide ISA-51 in Patients With Cancers Expressing the NY-ESO-1 Antigen2014
Author(s)
Wada H, Isobe M, Kakimi K, Mizote Y, Eikawa S, Sato E, Takigawa N, Kiura K, Tsuji K, Iwatsuki K, Yamasaki M, Miyata H, Matsushita H, Udono H, Seto Y, Yamada K, Nishikawa H, Pan L, Venhaus R, Oka M, Doki Y, Nakayama E.
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Journal Title
J Immunother
Volume: 37(2)
Issue: 2
Pages: 84-92
DOI
Related Report
Peer Reviewed
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[Presentation] 腹水貯留を伴う治療抵抗性胃癌に対するγδT細胞腹腔内投与治療の第1相臨床試験2013
Author(s)
和田 郁雄, 松下 博和, 垣見 和宏, 小野山 温那, 李 基成, 奥村 康弘, 谷島 翔, 神保 敬一, 土田 泰昭, 愛甲 丞, 山形 幸徳, 山下 裕玄, 森 和彦, 清水 伸幸, 野村 幸世, 瀬戸 泰之
Organizer
第113回日本外科学会定期学術集会
Place of Presentation
福岡県福岡市
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