Investigation of an EMAST-generating tumor microenvironment and its relating malignant progression for colorectal cancer
| Project/Area Number |
25462071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FUNAHASHI Kiminiko 東邦大学, 医学部, 教授 (90297698)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 癌微小環境 / 進行性大腸癌 / 低酸素 / 転移 / ゲノム不安定性 / EMAST / 癌 / MSH3 / p53 / 散発性大腸癌 / がん微小環境 |
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| Outline of Final Research Achievements |
The molecular mechanisms of progression and metastasis must be elucidated to cure metastatic colorectal cancers (CRCs). In this study, we established a new culture condition of colorectal cancer cell lines to generate elevated microsatellite alterations at selected tetra-nucleotide (EMAST). EMAST is a type of genomic instability and frequently detected in poor prognosis CRCs. Therefore, the in vitro EMAST generating condition is thought to reflect in vivo tumor microenvironment for malignant progression. Under this condition, we identified several genes with no apparent relevance to CRC by a comprehensive analysis of genes expression. Caveolin-1 (CAV1) was a candidate for novel malignant CRC-relating protein, because it was also detected in clinical CRC samples. Although precise roles of identified genes, including CAV1, were not reveled, our established EMAST-gerating culture condition was shown to be useful for a screening of malignant CRC-relating factors.
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Report
(3 results)
Research Products
(4 results)
