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Investigation of an EMAST-generating tumor microenvironment and its relating malignant progression for colorectal cancer

Research Project

Project/Area Number 25462071
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionToho University

Principal Investigator

ARITA Michitsune  東邦大学, 医学部, 助教 (80307719)

Co-Investigator(Renkei-kenkyūsha) FUNAHASHI Kiminiko  東邦大学, 医学部, 教授 (90297698)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords癌微小環境 / 進行性大腸癌 / 低酸素 / 転移 / ゲノム不安定性 / EMAST / 癌 / MSH3 / p53 / 散発性大腸癌 / がん微小環境
Outline of Final Research Achievements

The molecular mechanisms of progression and metastasis must be elucidated to cure metastatic colorectal cancers (CRCs). In this study, we established a new culture condition of colorectal cancer cell lines to generate elevated microsatellite alterations at selected tetra-nucleotide (EMAST). EMAST is a type of genomic instability and frequently detected in poor prognosis CRCs. Therefore, the in vitro EMAST generating condition is thought to reflect in vivo tumor microenvironment for malignant progression. Under this condition, we identified several genes with no apparent relevance to CRC by a comprehensive analysis of genes expression. Caveolin-1 (CAV1) was a candidate for novel malignant CRC-relating protein, because it was also detected in clinical CRC samples. Although precise roles of identified genes, including CAV1, were not reveled, our established EMAST-gerating culture condition was shown to be useful for a screening of malignant CRC-relating factors.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 Other

All Presentation (4 results)

  • [Presentation] Licochalcone A inhibits hypoxic and normoxic growth via different proliferative pathways in a human neuroblastoma cell line SK-N-SH2015

    • Author(s)
      有田通恒
    • Organizer
      第57回日本小児血液・がん学会学術集会
    • Place of Presentation
      甲府富士屋ホテル(山梨県・甲府市)
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 甘草由来成分リコカルコンAの抗がん活性:TrkB-JNK シグナリング阻害を介した神経芽腫細胞増殖抑制2015

    • Author(s)
      有田通恒
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Generation of EMAST in a colorectal cancer cell line under hypoxic condition

    • Author(s)
      Michitsune Arita
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Related Report
      2013 Research-status Report
  • [Presentation] N-myc非増幅神経芽腫細胞が示すMAPK経路活性化を介した低酸素性N-myc発現増加と増殖亢進

    • Author(s)
      有田通恒
    • Organizer
      第55回日本小児血液・がん学会学術集会
    • Place of Presentation
      ヒルトン福岡シーホーク(福岡)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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