Analysis of trans-differentiation genes in pancreatic intraepithelial neoplasia (PanIN)

• Project/Area Number: 25462110
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto University
• Principal Investigator: Takaori Kyoichi 京都大学, 学内共同利用施設等, 准教授 (10329485)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 膵腫瘍 / 膵癌 / 分化誘導関連遺伝子 / SOX9 / 膵管内乳頭状粘液性腫瘍 / IPMN

Outline of Final Research Achievements

Sex determining region Y-box 9 (SOX9) is a gene which plays a key role in the embryologic development, differentiation and maintenance of organs. In order to evaluate the potential role of SOX9 in intraductal papillary mucinous neoplasms (IPMNs) of the pancreas, we collected 27 pathological tissues from 19 IPMN cases and assessed the expression of SOX9 by immunohistochemistry in 78 lesions. At the results, SOX9 was expressed in the normal pancreas, IPMN and invasive carcinoma. SOX9 positive cells were confined to the lower portions of the papillary structures of IPMN. However, SOX9 was expressed in the entire epithelium once the neoplasms advanced to high-grade dysplasia and invasive carcinoma. The expression pattern of SOX9 was similar to that of CD44 in a normal pancreas and IPMN. Double staining detected co-localization of SOX9 and CD44 in IPMN. In conclusions, changes in the SOX9 expression pattern may be involved in the mechanisms of the malignant progression of IPMN.

Research Products

• [Journal Article] International Association of Pancreatology (IAP)/European Pancreatic Club (EPC) consensus review of guidelines for the treatment of pancreatic cancer. (2016)
  • Author(s): Takaori K, Bassi C, Biankin A, Brunner TB, Cataldo I, Campbell F, Cunningham D, Falconi M, Frampton AE, Furuse J, Giovannini M, Jackson R, Nakamura A, Nealon W, Neoptolemos JP, Real FX, Scarpa A, Sclafani F, Windsor JA, Yamaguchi K, Wolfgang C, Johnson CD
  • Journal Title: Pancreatology Volume: 16 Issue: 1 Pages: 14-27
  • DOI: 10.1016/j.pan.2015.10.013
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expression of SOX9 in intraductal papillary mucinous neoplasms of the pancreas (2014)
  • Author(s): Meng F, Takaori K, Ito T, Masui T, Kawaguchi M, Kawaguchi Y, Uemoto S.
  • Journal Title: Pancreas Volume: 43(1) Issue: 1 Pages: 7-14
  • DOI: 10.1097/mpa.0b013e3182a70b2f
  • Peer Reviewed