| Project/Area Number |
25462152
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
Kanki Sachiko 大阪医科大学, 医学部, 助教 (40411350)
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| Co-Investigator(Kenkyū-buntansha) |
渡邊 房男 大阪医科大学, 医学部, 准教授 (40183719)
三重野 繁敏 大阪医科大学, 医学部, 非常勤講師 (10411373)
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| Co-Investigator(Renkei-kenkyūsha) |
MIENO Shigetoshi 大阪医科大学, 医学部, 非常勤講師 (10411373)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 虚血性心筋症 / ホーミングペプチド / ペプチド創薬 / 培養細胞 / プロテオーム解析 / 虚血再灌流傷害 / 培養心筋細胞 / 遺伝子導入 / 心筋虚血 / 質量分析 / ペプチド |
|---|
| Outline of Final Research Achievements |
To identify the mechanism of the ischemic myocardium targeting peptide that was found by in vivo phage display in rats, we performed the pull down assay with the peptide -tagged nano magnetic beads in the rats' ischemic myocardium. We harvested 10 bands from a gel-electrophoresis and analyzed them with mass finger printing by mass-sectrometory combined with MALDI-TOF. We obtained 5 candidate proteins. In order to verify whether these proteins are receptors of the homing peptide, we constructed recombinant candidate proteins that are tagged with fluorescence. To establish in vitro ischemia-reperfusion condition with cells, we succeeded to use a chemical condition with sodium cyanide instead of with a hypoxic chamber. We confirmed the cell damage by LDH release and ATP generation by the cells. Although the chemical ischemia-reperfusion worked and mimic the in vivo ischemia-reperfusion, the cells did not absorb the fluorecence-tagged homing peptide.
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