Mechanism identification and Developing a novel method for therapy against lung cancer invasion via cancer-associated fibroblasts in pulmonary emphysema

Research Project

Project/Area Number	25462177
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Respiratory surgery
Research Institution	Yamaguchi University
Principal Investigator	HAYASHI Masataro 山口大学, 医学部附属病院, 助教 (00554057)
Co-Investigator(Kenkyū-buntansha)	NISHIMOTO Arata 山口大学, 医学部附属病院, 助教 (90396325) UEDA Kazuhiro 山口大学, 医学部附属病院, 講師 (90420520)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	肺癌 / 肺気腫 / 癌関連線維芽細胞 / 肺気腫合併肺癌
Outline of Final Research Achievements	To evaluate whether cancer-related fibroblasts are useful as a novel target for lung cancer therapy, we aimed to elucidate the characteristic of cancer-related fibroblasts in lung cancer with pulmonary emphysema and the role of cancer-related fibroblasts in lung cancer progression in the emphysema lung. We manufactured Lewis lung cancer xenograft in murine model with pulmonary emphysema and tried to identify cancer-related fibroblasts in the emphysema lung resected from this model by detecting FAP and alpha-SMA-positive cells using FACS analysis.