New treatment strategy for malignant pleural mesothelioma focusing on microRNAs related to cancer stem cell makers

• Project/Area Number: 25462195
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Okayama University
• Principal Investigator: SOH JUNICHI 岡山大学, 大学病院, 講師 (90559890)
• Co-Investigator(Kenkyū-buntansha): MIYOSHI Shinichiro 岡山大学, 医歯(薬)学総合研究科, 教授 (00190827) ; TSUKUDA Kazunori 岡山大学, 大学病院, 講師 (20346430) ; TOYOOKA Shinichi 岡山大学, 医歯(薬)学総合研究科, 教授 (30397880) ; YAMAMOTO Hiromasa 岡山大学, 大学病院, 助教 (40467733) ; ASANO Hiroaki 岡山大学, 大学病院, 助教 (70534775)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 悪性胸膜中皮腫 / 癌幹細胞 / マイクロRNA

Outline of Final Research Achievements

Malignant pleural mesothelioma (MPM) is a well-known tumor which shows treatment resistance and dismal prognosis. There is a need to resolve the cause of this disease and to develop a new treatment. We investigated the cause of MPM and the new treatment strategy by focusing on the cancer stem cell and micro-RNA (moRNA) related to cancer stem cell markers. We performed a comprehensive miRNA expression analysis to find the families of miRNA-34 and miRNA-200 as candidate of cancer stem cell related miRNA of MPM. We analyzed the effect of transduction of these miRNAs on expression of cancer stem cell related makers, cell viability, and drug sensitivity. We are investigating the new candidates of miRNAs relaeted to cancer stem cell of MPM by cell sorting method.

Research Products

• [Journal Article] Establishment and molecular characterization of cell lines from Japanese patients with malignant pleural mesothelioma. (2016)
  • Author(s): Suzawa K, Yamamoto H, Murakami T, Katayama H, Furukawa M, Shien K, Hashida S, Okabe K, Aoe K, Soh J, Asano H, Tsukuda K, Mimura Y, Toyooka S, Miyoshi S
  • Journal Title: Oncol Lett Volume: 1 Issue: 1 Pages: 705-712
  • DOI: 10.3892/ol.2015.3955
  • Peer Reviewed
• [Presentation] 悪性胸膜中皮腫に対するREIC/Dkk-3遺伝子治療 (2015)
  • Author(s): 山本寛斉、枝園和彦、牧佑歩、宗淳一、大谷真二、三好健太郎、杉本誠一郎、山根正修、大藤剛宏、渡部昌実、那須保友、公文裕巳、三好新一郎、豊岡伸一
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2015-11-26