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In vivo MR imaging of microglia in glioblastoma

Research Project

Project/Area Number 25462256
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurosurgery
Research InstitutionResearch Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses

Principal Investigator

KINOSHITA MANABU  地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), 脳神経外科, 医長 (40448064)

Co-Investigator(Kenkyū-buntansha) YOSHIOKA Yoshichika  大阪大学, 免疫学フロンティア研究センター, 特任教授(常勤) (00174897)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsMRI / 膠芽腫 / 細胞浸潤 / in vivo イメージング / 分子イメージング
Outline of Final Research Achievements

A promising MR reporter gene, MagA was used to attempt microglia imaging in glioblastoma in a murine brain tumor model. A chimera MagA transgenic mouse was successfully established and a glioblastoma brain tumor model was produced by implanting murine glioblastoma cells into the brain. Detection of microglia, or any MagA positive cells, however, on MRI was unsuccessful. Taking into account new data published from other institutions, it is speculated that cell toxicity of MagA in murine cell lines interfered with the expression level of MagA within the cells that was targeted to be reported on MRI. On the other hand, our research was able to develop novel image analyzing techniques to identify the tumor heterogeneity and tumor boarder diffuseness using MRI, which characteristics reflect tumor cell biology of gliomas.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (30 results)

All 2016 2015 2014 2013 Other

All Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Remarks (3 results)

  • [Journal Article] Diagnostic and prognostic value of 11C-methionine positron emission tomography for MR non-enhancing glioma.2016

    • Author(s)
      Takano K, Kinoshita M, Arita H, Okita Y, Chiba Y, Kagawa N, Fujimoto Y, Kishima H, Kanemura Y, Nonaka M, Nakajima S, Shimosegawa E, Hatazawa J, Hashimoto N, Yoshimine T
    • Journal Title

      AJNR Am J Neuroradiol

      Volume: -

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Different spatial distribution of brain metastases from lung cancer by histological subtypes and EGFR mutation status.2016

    • Author(s)
      Takano K, Kinoshita M, Takagaki M, Sakai M, Tateishi S, Achiha T, Hirayama R, Nishino K, Uchida J, Kumagai T, Okami J, Kawaguchi A, Hashimoto N, Nakanishi K, Imamura F, Higashiyama M, Yoshimine T
    • Journal Title

      Neuro-oncology

      Volume: 18 Issue: 5 Pages: 716-724

    • DOI

      10.1093/neuonc/nov266

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of diffusion tensor imaging and 11C-methionine positron emission tomography for reliable prediction of tumor cell density in gliomas.2016

    • Author(s)
      Kinoshita M, Arita H, Okita Y, Kagawa N, Kishima H, Hashimoto N, Tanaka H, Watanabe Y, Shimosegawa E, Hatazawa J, Fujimoto Y, Yoshimine T
    • Journal Title

      J Neurosurg

      Volume: 印刷中 Issue: 5 Pages: 1136-1142

    • DOI

      10.3171/2015.11.jns151848

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of a non-tissue adherent neurosurgical patty and an ex vivo testing system to evaluate adherent characteristics2015

    • Author(s)
      Manabu Kinoshita
    • Journal Title

      Journal of Neurosurgery

      Volume: 122 Issue: 5 Pages: 1180-1184

    • DOI

      10.3171/2014.9.jns14347

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pituitary-Targeted Dynamic Contrast-Enhanced Multisection CT for Detecting MR Imaging-Occult Functional Pituitary Microadenoma2015

    • Author(s)
      Kinoshita M, Tanaka H, Arita H, Goto Y, Oshino S, Watanabe Y, Yoshimine T, Saitoh Y.
    • Journal Title

      AJNR Am J Neuroradiol.

      Volume: 36 Issue: 5 Pages: 904-908

    • DOI

      10.3174/ajnr.a4220

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Wilms tumor 1 peptide vaccination combined with temozolomide against newly diagnosed glioblastoma: safety and impact on immunological response2015

    • Author(s)
      Naoya Hashimoto, Akihiro Tsuboi, Naoki Kagawa, Yasuyoshi Chiba, Shuichi Izumoto, Manabu Kinoshita, Noriyuki Kijima, Yoshihiro Oka, Soyoko Morimoto, Hiroko Nakajima, Satoshi Morita, Junichi Sakamoto, Sumiyuki Nishida, Naoki Hosen, Yusuke Oji, Norio Arita, Toshiki Yoshimine, Haruo Sugiyama
    • Journal Title

      Cancer Immunology Immunotherapy

      Volume: - Issue: 6 Pages: 707-16

    • DOI

      10.1007/s00262-015-1674-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Recurrent mutations of CD79B and MYD88 are the hallmark of primary central nervous system lymphomas.2015

    • Author(s)
      Nakamura T, Tateishi K, Niwa T, Matsushita Y, Tamura K, Kinoshita M, Tanaka K, Fukushima S, Takami H, Arita H, Kubo A, Shuto T, Ohno M, Miyakita Y, Kocialkowski S, Sasayama T, Hashimoto N, Maehara T, Shibui S, Ushijima T, Kawahara N, Narita Y, Ichimura K.
    • Journal Title

      Neuropathol Appl Neurobiol.

      Volume: 42(3) Issue: 3 Pages: 279-290

    • DOI

      10.1111/nan.12259

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Olfactory is an overlooked site for the initiation of cerebral malaria.2014

    • Author(s)
      Zhao H, Aoshi T, Kawai S, Mori Y, Fujita Y, Konishi A, Shimizu M, Kohyama M, Kobiyama K, Eto K, Nabekura J, Horii T, Ishino T, Yuda M, Hemmi H, Kaisho T, Akira S, Kinoshita M, Tohyama K, Yoshioka Y, Ishii KJ, Coban C
    • Journal Title

      Cell Host Microbe

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 2.Different spatial distribution between germinal center B and non-germinal center B primary central nervous system lymphoma revealed by magnetic resonance group analysis.2014

    • Author(s)
      Kinoshita M1, Sasayama T, Narita Y, Yamashita F, Kawaguchi A, Chiba Y, Kagawa N, Tanaka K, Kohmura E, Arita H, Okita Y, Ohno M, Miyakita Y, Shibui S, Hashimoto N, Yoshimine T
    • Journal Title

      Neuro Oncol

      Volume: 16 Issue: 5 Pages: 728-734

    • DOI

      10.1093/neuonc/not319

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Wilms' tumor 1 is involved in tumorigenicity of glioblastoma by regulating cell proliferation and apoptosis.2014

    • Author(s)
      Kijima N, Hosen N, Kagawa N, Hashimoto N, Kinoshita M, Oji Y, Sugiyama H, Yoshimine T
    • Journal Title

      Anticancer Res

      Volume: 34 Pages: 61-67

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gsp mutation in acromegaly and its influence on TRH-induced paradoxical GH response.2014

    • Author(s)
      Goto Y, Kinoshita M, Oshino S, Arita H, Kitamura T, Ostuki M, Shimomura I, Yoshimine T, Saitoh Y
    • Journal Title

      Clin Endocrinol (Oxf)

      Volume: 80 Issue: 5 Pages: 714-9

    • DOI

      10.1111/cen.12336

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hypofractionated stereotactic radiation therapy in three to five fractions for vestibular schwannoma.2013

    • Author(s)
      Morimoto M, Yoshioka Y, Kotsuma T, Adachi K, Shiomi H, Suzuki O, Seo Y, Koizumi M, Kagawa N, Kinoshita M, Hashimoto N, Ogawa K
    • Journal Title

      Jpn J Clin Oncol

      Volume: 43 Issue: 8 Pages: 805-12

    • DOI

      10.1093/jjco/hyt082

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ダイナミック造影マルチスライスCTに対する造影動態解析ソフトウェアの開発と機能性微小下垂体腺腫診断への臨床応用について2016

    • Author(s)
      木下学、田中壽、有田英之、後藤雄子、押野悟、渡邊嘉之、吉峰俊樹、齋藤洋一
    • Organizer
      第26回日本間脳下垂体腫瘍学会
    • Place of Presentation
      ザ・セレクトン福島(福島県福島市)
    • Year and Date
      2016-02-19
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] MRI による神経膠腫の網羅的画像解析法:大規模コホート解析に向けての提案2015

    • Author(s)
      木下学、有田英之、酒井美緒、香川尚己、金村米博、藤本康倫、中西克之、吉峰俊樹
    • Organizer
      第33回日本脳腫瘍学会
    • Place of Presentation
      グランドプリンスホテル京都(京都)
    • Year and Date
      2015-12-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Different spatial distribution of brain metastasis from lung cancer defined by EGFR mutation status2015

    • Author(s)
      Takano K, Kinoshita M, Takagaki M, Sakai M, Nishino K, Uchida J, Kumagai T, Okami J, Imamura F, Higashiyama M, Hashimoto N, Yoshimine T
    • Organizer
      2015 Society for Neuro-onclogy (SNO) meeting
    • Place of Presentation
      MARRIOTT RIVERCENTER HOTEL(San Antonio TX, USA)
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel PET index, 11C-Methionine/18F-FDG uptake decoupling score, in malignant glioma: Evaluation of its geometrical features for planning radiation therapy2015

    • Author(s)
      Hirata T, Kinoshita M, Arita H, Suzuki O, Hashimoto N, Hatazawa J, Toshiba T, Yoshimine T, Ozawa K
    • Organizer
      2015 Society for Neuro-onclogy (SNO) meeting
    • Place of Presentation
      MARRIOTT RIVERCENTER HOTEL(San Antonio TX, USA)
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 標準脳を用いた中枢神経系悪性リンパ腫の脳内局在解析 脳内発生部位の観点からみた 中枢神経系悪性リンパ腫の疾患不均一性の検討2015

    • Author(s)
      木下学、篠山隆司、成田善孝、有田英之、香川尚己、山下典生、川口淳、橋本直哉、吉峰俊樹
    • Organizer
      日本脳神経外科学会第74回学術総会
    • Place of Presentation
      ロイトン札幌ほか(北海道札幌市)
    • Year and Date
      2015-10-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Brain tumor image analysis using voxel-based MR group analysis2015

    • Author(s)
      Kinoshita M, Koji Takano, Takashi Sasayama, Yoshitaka Narita, Fumio Imamura, Naoya Hashimoto, Toshiki Yoshimine
    • Organizer
      第30回日本脳神経外科国際フォーラム
    • Place of Presentation
      兵庫県立淡路夢舞台国際会議場 (兵庫県淡路市)
    • Year and Date
      2015-07-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 多段階手術により摘出し得た大型充実性血管芽腫の一例2015

    • Author(s)
      木下 学、高垣匡寿、中村 元、阿知波孝宗、中西克彦、吉峰俊樹
    • Organizer
      第79回大阪大学脳神経外科関連施設臨床懇話会
    • Place of Presentation
      大阪大学(大阪府吹田市)
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Comparison of DTI and 11C-methionine PET for reliable prediction of tumor cell density in gliomas2015

    • Author(s)
      Kinoshita M, Hideyuki Arita, Naoki Kagawa, Yoshiyuki Watanabe, Jun Hatazawa, Naoya Hashimoto, Toshiki Yoshimine
    • Organizer
      International Society for Magnetic Resonance in Medicine 2015
    • Place of Presentation
      Metro Toronto Convention Centre(Toronto, Canada)
    • Year and Date
      2015-06-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Different spatial distribution between GCB and non-GCB PCNSL revealed by MR group analysis of 100 cases.2013

    • Author(s)
      Kinoshita M, Sasayama T, Narita Y, Kwaguchi A, Yamashita F, Chiba Y, Kagawa N, Tanaka K, Kohmura E, Arita H, Okita Y, Ohon M, Miyakita Y, Shizui S, Hashimoto N, Yoshimine T
    • Organizer
      The 4th Quadrennial Meeting of the World Federation of Neuro-Oncology.
    • Place of Presentation
      サンフランシスコ
    • Related Report
      2013 Research-status Report
  • [Presentation] 中枢神経系悪性リンパ腫のサブタイプによる発生部位の相違について ~MRIグルーフ解析法による連続100例の解析結果~2013

    • Author(s)
      木下学、篠山隆司、成田善孝、山下典生、川口淳、千葉泰良、香川尚己、田中一寛、甲村英二、有田英之、沖田典子、大野誠、宮北康二、澁井壮一郎、橋本直哉、吉峰俊樹
    • Organizer
      第29回日本脳腫瘍学会
    • Place of Presentation
      宮崎
    • Related Report
      2013 Research-status Report
  • [Presentation] マルチモダール画像/組織ライブラリー構築のためのナビゲーション解析ソフトウエアの開発~マルチモダール手術の検証を目指して~2013

    • Author(s)
      木下学、高野浩司、沖田典子、千葉泰良、香川尚己、橋本直哉、吉峰俊樹
    • Organizer
      第72回日本脳神経外科学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] Dynamiccontrast-enhanced CTによるmicroadenomaの描出

    • Author(s)
      木下学、有田英之、田中壽、後藤雄子、渡邉嘉之、押野悟、齋藤洋一
    • Organizer
      第43回日本神経放射線学会
    • Place of Presentation
      米子
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Dynamiccontrast-enhanced CTによるmicroadenomaの描出

    • Author(s)
      木下学、有田英之、田中壽、後藤雄子、渡邉嘉之、押野悟、齋藤洋一
    • Organizer
      日本脳神経CI学会総会
    • Place of Presentation
      大宮
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 4D造影CTによる機能性微小下垂体腺腫の画像診断

    • Author(s)
      木下学、有田英之、田中壽、後藤雄子、押野悟、渡邉嘉之、齋藤洋一
    • Organizer
      第24回日本間脳下垂体腫瘍学会
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 悪性脳腫瘍手術におけるマルチモダール手術支援技術の検証

    • Author(s)
      木下学、有田英之、沖田典子、千葉泰良、香川尚己、畑澤順、橋本直哉、吉峰俊樹
    • Organizer
      第18回日本脳腫瘍の外科学会
    • Place of Presentation
      大津
    • Related Report
      2013 Research-status Report
  • [Remarks] 大阪府立成人病センター脳神経外科ホームページ

    • Related Report
      2015 Annual Research Report
  • [Remarks] 研究者向け研究内容紹介

    • Related Report
      2015 Annual Research Report
  • [Remarks] ホームページ

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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