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microRNA aberration in gliomas with IDH mutation and metastasis

Research Project

Project/Area Number 25462258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurosurgery
Research InstitutionKobe University

Principal Investigator

Sasayama Takashi  神戸大学, 医学部附属病院, 講師 (10379399)

Co-Investigator(Kenkyū-buntansha) KOHMURA Eiji  神戸大学, 医学(系)研究科(研究院), 教授 (30225388)
TANAKA Kazuhiro  神戸大学, 医学(系)研究科(研究院), 研究員 (70467661)
MIZUKAWA Katsu  神戸大学, 医学部附属病院, 助教 (80403260)
NISHIHARA Masamitsu  神戸大学, 医学(系)研究科(研究院), 研究員 (20452493)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsglioma / microRNA / IDH mutation / metastatic / IDH / グリオーマ / マイクロRNA
Outline of Final Research Achievements

To generate glioma cells expressing isocitrate dehydrogenase-1 (IDH1) mutant protein, we cultured glioma cells from the glioma tissues that have IDH1 mutant. But, glioma cells expressing IDH1 mutant protein could not grow. When plasmides IDH1 mutant were transfected in glioma cells, glioma cell lines that stably express IDH1 mutant protein could not grow. In glioma cells that transiently express IDH1 mutant, 2HG was significantly elevated and glutamate was significantly reduced in glioma cells. Then, we analyzed microRNA expression in disseminated or metastatic glioma. In disseminated/metastatic gliomas, miR-7、miR-29b、miR-34a、miR-101、miR-124、miR-128a、miR-137、mR-218 were significantly down-regulated compared with non-metastatic gliomas. When these microRNAs' mimics were transfected in glioma cells, glioma cell migration, proliferation, and invasion were decreased. Therefore, these microRNAs could be predictive biomarker for disseminated/metastatic glioma.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (20 results)

All 2016 2015 2014 2013

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Radiographic occult cerebellar germinoma presenting with progressive ataxia and cranial nerve palsy.2016

    • Author(s)
      Minami N, Tanaka K, Kimura H, Hirose T, Mori T, Maeyama M, Sekiya H, Uenaka T, Nakamizo S, Nagashima H, Mizukawa K, Itoh T, Sasayama T, Kohmura E.
    • Journal Title

      BMC Neurol.

      Volume: 16 Issue: 1 Pages: 1-4

    • DOI

      10.1186/s12883-015-0516-9

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Radiotherapy for brainstem gliomas in children and adults: A single-institution experience and literature review.2016

    • Author(s)
      Yoshida K, Sulaiman NS, Miyawaki D, Ejima Y, Nishimura H, Ishihara T, Matsuo Y, Nishikawa R, Sasayama T, Hayakawa A, Kohmura E, Sasaki R
    • Journal Title

      Asia Pac J Clin Oncol.

      Volume: Mar 3 Issue: 2 Pages: 1-8

    • DOI

      10.1111/ajco.12451

    • NAID

      120006375924

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two Cases of Cerebral Infarction due to Focal Irradiation for Glioma in Adults2015

    • Author(s)
      Minami N, Mizukawa K, Iwahashi H, Nagashima H, Tanaka K, Sasayama T, Hosoda K, Mukumoto N, Sasaki R, Kohmura E.
    • Journal Title

      Neurological Surgery 脳神経外科

      Volume: 43 Issue: 4 Pages: 344-351

    • DOI

      10.11477/mf.1436203018

    • ISSN
      0301-2603, 1882-1251
    • Year and Date
      2015-04-10
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Compensatory glutamine metabolism promotes glioblastoma resistance to mTOR inhibitor treatment.2015

    • Author(s)
      Tanaka K, Sasayama T, Irino Y, Takata K, Nagashima H, Satoh N, Kyotani K, Mizowaki T, Imahori T, Ejima Y, Masui K, Gini B, Yang H, Hosoda K, Sasaki R, Mischel PS, Kohmura E.
    • Journal Title

      J Clin Invest

      Volume: 125 Issue: 4 Pages: 1591-602

    • DOI

      10.1172/jci78239

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] STAT3 activation is associated with cerebrospinal fluid interleukin-10 (IL-10) in primary central nervous system diffuse large B cell lymphoma.2015

    • Author(s)
      Mizowaki T, Sasayama T, Tanaka K, Mizukawa K, Takata K, Nakamizo S, Tanaka H, Nagashima H, Nishihara M, Hirose T, Itoh T, Kohmura E
    • Journal Title

      J Neurooncol

      Volume: 124(2) Issue: 2 Pages: 165-74

    • DOI

      10.1007/s11060-015-1843-9

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A 40-Year-Old Female with Leptomeningeal Lesions.2015

    • Author(s)
      Mizowaki T, Sasayama T, Semba S, Sasaki R, Yasuo K, Nakamizo S, Tanaka K, Mizukawa K, Uchihashi Y, Kohmura E.
    • Journal Title

      Brain Pathol.

      Volume: 25 Issue: 4 Pages: 509-10

    • DOI

      10.1111/bpa.12270

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Combined IDH1 mutation and MGMT methylation status on long-term survival of patients with cerebral low-grade glioma.2015

    • Author(s)
      Tanaka K, Sasayama T, Mizukawa K, Takata K, Sulaiman NS, Nishihara M, Kohta M, Sasaki R, Hirose T, Itoh T, Kohmura E.
    • Journal Title

      Clin Neurol Neurosurg.

      Volume: 138 Pages: 37-44

    • DOI

      10.1016/j.clineuro.2015.07.019

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tumor-Associated Macrophages Associate with Cerebrospinal Fluid Interleukin-10 and Survival in Primary Central Nervous System Lymphoma (PCNSL)2015

    • Author(s)
      Sasayama T, Tanaka K, Mizowaki T, Nagashima H, Nakamizo S, Tanaka H, Nishihara M, Mizukawa K, Hirose T, Itoh T, Kohmura E
    • Journal Title

      Brain Pathol.

      Volume: Aug 28 Issue: 4 Pages: 1-9

    • DOI

      10.1111/bpa.12318

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeting glutaminase and mTOR.2015

    • Author(s)
      Tanaka K, Sasayama T, Kohmura E.
    • Journal Title

      Oncotarget.

      Volume: 6(29) Issue: 29 Pages: 26544-26545

    • DOI

      10.18632/oncotarget.5263

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 2.Different spatial distribution between germinal center B and non-germinal center B primary central nervous system lymphoma revealed by magnetic resonance group analysis.2014

    • Author(s)
      Kinoshita M1, Sasayama T, Narita Y, Yamashita F, Kawaguchi A, Chiba Y, Kagawa N, Tanaka K, Kohmura E, Arita H, Okita Y, Ohno M, Miyakita Y, Shibui S, Hashimoto N, Yoshimine T
    • Journal Title

      Neuro Oncol

      Volume: 16 Issue: 5 Pages: 728-734

    • DOI

      10.1093/neuonc/not319

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article]2013

    • Author(s)
      Nakamizo S, Sasayama T, Shinohara M, Irino Y, Nishiumi S, Nishihara M, Tanaka H, Tanaka K, Mizukawa K, Itoh T, Taniguchi M, Hosoda K, Yoshida M, Kohmura E
    • Journal Title

      J Neurooncol

      Volume: 113(1) Issue: 1 Pages: 65-74

    • DOI

      10.1007/s11060-013-1090-x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article]2013

    • Author(s)
      Tanaka H, Sasayama T, Tanaka K, Nakamizo S, Nishihara M, Mizukawa K, Kohta M, Koyama J, Miyake S, Taniguchi M, Hosoda K, Kohmura E
    • Journal Title

      J Neurooncol

      Volume: 111(3) Issue: 3 Pages: 273-83

    • DOI

      10.1007/s11060-012-1027-9

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article]2013

    • Author(s)
      Ishii T, Mizukawa K, Sasayama T, Sasaki H, Hayashi S, Nakamizo S, Tanaka H, Tanaka K, Hara S, Hirai C, Itoh T, Kohmura E
    • Journal Title

      Neuropathology

      Volume: 33(3) Issue: 3 Pages: 299-305

    • DOI

      10.1111/j.1440-1789.2012.01349.x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article]2013

    • Author(s)
      Taniguchi M, Nishihara M, Sasayama T, Takahashi Y, Kohmura E
    • Journal Title

      Neuropathol Appl Neurobiol

      Volume: 39(4) Issue: 4 Pages: 445-8

    • DOI

      10.1111/nan.12000

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] De-repression of PDGFRβ transcription promotes acquired resistance to EGFR tyrosine kinase inhibitors in glioblastoma patients.2013

    • Author(s)
      Akhavan D, Pourzia AL, Nourian AA, Williams KJ, Nathanson D, Babic I, Villa GR, Tanaka K, Nael A, Yang H, Dang J, Vinters HV, Yong WH, Flagg M, Tamanoi F, Sasayama T, James CD, Kornblum HI, Cloughesy TF, Cavenee WK, Bensinger SJ, Mischel PS.
    • Journal Title

      Cancer Discov

      Volume: 3 Issue: 5 Pages: 534-47

    • DOI

      10.1158/2159-8290.cd-12-0502

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] The characteristics of microRNA expressions in glioblastoma with leptomeningeal dissemination or metastasis2015

    • Author(s)
      Takashi Sasayama1), Kazuhiro Tanaka1), Mitsutoshi Nakada2), Naoya Hashimoto3), Manabu Kinoshita3), Naoki Kagawa3), Katsu Mizukawa1), Hirotomo Tanaka1), Takashi Miaowaki1), Hiroaki Nagashima1), Junichi Sakata1), Eiji Kohmura1)
    • Organizer
      Society for Neuro-Oncology Annual Scientific Meeting
    • Place of Presentation
      San Antonio
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cerebrospinal fluid interleukin-10 is a potentially useful biomarker in immunocompetent primary centralnervous system lymphoma (PCNSL)2013

    • Author(s)
      Takashi Sasayama, Masamitsu Nishihara , Kazuhiro Tanaka, Katsu Mizukawa, Satoshi Nakamizo, Hirotomo Tanaka, Eiji Kohmura
    • Organizer
      BIT’s 6th World cancer conference 2013
    • Place of Presentation
      Xi’an
    • Related Report
      2013 Research-status Report
  • [Presentation] The combination of interleukin-10 and b2-microglobulin in Cerebrospinal fluid is a useful biomarker in immunocompetent primary central nervous system lymphoma (PCNSL)2013

    • Author(s)
      Takashi Sasayama, Kazuhiro Tanaka, Satoshi Nakamizo, Masamitsu Nishihara, Atsufumi Kawamura, Hirotomo Tanaka, Katsu Mizukawa, Takashi Mizowaki, Kohkichi Hosoda, Eiji Kohmura
    • Organizer
      4th Quadrennial Meeting of the World Federation of Neuro-Oncology
    • Place of Presentation
      San Francisco
    • Related Report
      2013 Research-status Report
  • [Presentation] 脊髄播種・転移を起こした膠芽腫の臨床的・組織学的検討2013

    • Author(s)
      篠山隆司、田中一寛、水川克、溝脇卓、長嶋宏明、堀達雄、甲村英二
    • Organizer
      第31回日本脳腫瘍学会
    • Place of Presentation
      宮崎
    • Related Report
      2013 Research-status Report
  • [Book] Primary Central Nervous System Lymphoma (PCNSL): Incidence, Management and Outcomes2016

    • Author(s)
      Takashi Sasayama1,*, Kazuhiro Tanaka1, Katsu Mizukawa1, Masamitsu Nishihara2 and Eiji Kohmura1
    • Total Pages
      16
    • Publisher
      NOVA SCIENTIFIC PUBLISHERS, INC
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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