microRNA aberration in gliomas with IDH mutation and metastasis
Project/Area Number |
25462258
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurosurgery
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Research Institution | Kobe University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KOHMURA Eiji 神戸大学, 医学(系)研究科(研究院), 教授 (30225388)
TANAKA Kazuhiro 神戸大学, 医学(系)研究科(研究院), 研究員 (70467661)
MIZUKAWA Katsu 神戸大学, 医学部附属病院, 助教 (80403260)
NISHIHARA Masamitsu 神戸大学, 医学(系)研究科(研究院), 研究員 (20452493)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | glioma / microRNA / IDH mutation / metastatic / IDH / グリオーマ / マイクロRNA |
Outline of Final Research Achievements |
To generate glioma cells expressing isocitrate dehydrogenase-1 (IDH1) mutant protein, we cultured glioma cells from the glioma tissues that have IDH1 mutant. But, glioma cells expressing IDH1 mutant protein could not grow. When plasmides IDH1 mutant were transfected in glioma cells, glioma cell lines that stably express IDH1 mutant protein could not grow. In glioma cells that transiently express IDH1 mutant, 2HG was significantly elevated and glutamate was significantly reduced in glioma cells. Then, we analyzed microRNA expression in disseminated or metastatic glioma. In disseminated/metastatic gliomas, miR-7、miR-29b、miR-34a、miR-101、miR-124、miR-128a、miR-137、mR-218 were significantly down-regulated compared with non-metastatic gliomas. When these microRNAs' mimics were transfected in glioma cells, glioma cell migration, proliferation, and invasion were decreased. Therefore, these microRNAs could be predictive biomarker for disseminated/metastatic glioma.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Radiotherapy for brainstem gliomas in children and adults: A single-institution experience and literature review.2016
Author(s)
Yoshida K, Sulaiman NS, Miyawaki D, Ejima Y, Nishimura H, Ishihara T, Matsuo Y, Nishikawa R, Sasayama T, Hayakawa A, Kohmura E, Sasaki R.
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Journal Title
Asia Pac J Clin Oncol
Volume: Mar 3
Pages: 1-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Compensatory glutamine metabolism promotes glioblastoma resistance to mTOR inhibitor treatment.2015
Author(s)
Tanaka K, Sasayama T, Irino Y, Takata K, Nagashima H, Satoh N, Kyotani K, Mizowaki T, Imahori T, Ejima Y, Masui K, Gini B, Yang H, Hosoda K, Sasaki R, Mischel PS, Kohmura E.
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Journal Title
J Clin Invest.
Volume: 125(4)
Pages: 1591-602
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] STAT3 activation is associated with cerebrospinal fluid interleukin-10 (IL-10) in primary central nervous system diffuse large B cell lymphoma.2015
Author(s)
Mizowaki T, Sasayama T, Tanaka K, Mizukawa K, Takata K, Nakamizo S, Tanaka H, Nagashima H, Nishihara M, Hirose T, Itoh T, Kohmura E.
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Journal Title
J Neurooncol
Volume: 124(2)
Pages: 165-74
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Combined IDH1 mutation and MGMT methylation status on long-term survival of patients with cerebral low-grade glioma.2015
Author(s)
Tanaka K, Sasayama T, Mizukawa K, Takata K, Sulaiman NS, Nishihara M, Kohta M, Sasaki R, Hirose T, Itoh T, Kohmura E.
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Journal Title
Clin Neurol Neurosurg
Volume: 138
Pages: 37-44
DOI
Related Report
Peer Reviewed
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[Journal Article] Tumor-Associated Macrophages Associate with Cerebrospinal Fluid Interleukin-10 and Survival in Primary Central Nervous System Lymphoma (PCNSL).2015
Author(s)
Sasayama T, Tanaka K, Mizowaki T, Nagashima H, Nakamizo S, Tanaka H, Nishihara M, Mizukawa K, Hirose T, Itoh T, Kohmura E.
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Journal Title
Brain Pathol
Volume: Aug 28
Pages: 1-9
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Radiographic occult cerebellar germinoma presenting with progressive ataxia and cranial nerve palsy.2015
Author(s)
Minami N, Tanaka K, Kimura H, Hirose T, Mori T, Maeyama M, Sekiya H, Uenaka T, Nakamizo S, Nagashima H, Mizukawa K, Itoh T, Sasayama T, Kohmura E.
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Journal Title
BMC Neurol
Volume: 16(1)
Pages: 4-4
DOI
Related Report
Peer Reviewed
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[Journal Article] Different spatial distribution between germinal center B and non-germinal center B primary central nervous system lymphoma revealed by magnetic resonance group analysis2014
Author(s)
Kinoshita M, Sasayama T, Narita Y, Yamashita F, Kawaguchi A, Chiba Y, Kagawa N, Tanaka K, Kohmura E, Arita H, Okita Y, Ohno M, Miyakita Y, Shibui S, Hashimoto N, Yoshimine T.
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Journal Title
Neuro Oncol
Volume: 16
Pages: 728-34
DOI
Related Report
Peer Reviewed
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[Journal Article] GC/MS-based metabolomic analysis of cerebrospinal fluid (CSF) from glioma patients.2013
Author(s)
Nakamizo S, Sasayama T, Shinohara M, Irino Y, Nishiumi S, Nishihara M, Tanaka H, Tanaka K, Mizukawa K, Itoh T, Taniguchi M, Hosoda K, Yoshida M, Kohmura E.
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Journal Title
J Neurooncol
Volume: 113
Pages: 65-74
DOI
Related Report
Peer Reviewed
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[Journal Article] MicroRNA-183 upregulates HIF-1α by targeting isocitrate dehydrogenase 2 (IDH2) in glioma cells.2013
Author(s)
Tanaka H, Sasayama T, Tanaka K, Nakamizo S, Nishihara M, Mizukawa K, Kohta M, Koyama J, Miyake S, Taniguchi M, Hosoda K, Kohmura E.
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Journal Title
J Neurooncol
Volume: 111
Pages: 273-83
DOI
Related Report
Peer Reviewed
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[Journal Article] Immunohistochemical and molecular genetics study of a granular cell astrocytoma: a case report of malignant transformation to a glioblastoma2013
Author(s)
Ishii T, Mizukawa K, Sasayama T, Sasaki H, Hayashi S, Nakamizo S, Tanaka H, Tanaka K, Hara S, Hirai C, Itoh T, Kohmura E
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Journal Title
Neuropathology
Volume: 33
Pages: 299-305
DOI
Related Report
Peer Reviewed
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[Journal Article] De-repression of PDGFRβ transcription promotes acquired resistance to EGFR tyrosine kinase inhibitors in glioblastoma patients.2013
Author(s)
Akhavan D, Pourzia AL, Nourian AA, Williams KJ, Nathanson D, Babic I, Villa GR, Tanaka K, Nael A, Yang H, Dang J, Vinters HV, Yong WH, Flagg M, Tamanoi F, Sasayama T, James CD, Kornblum HI, Cloughesy TF, Cavenee WK, Bensinger SJ, Mischel PS.
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Journal Title
Cancer Discov
Volume: 3
Pages: 534-47
DOI
Related Report
Peer Reviewed
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[Presentation] The characteristics of microRNA expressions in glioblastoma with leptomeningeal dissemination or metastasis2015
Author(s)
Takashi Sasayama1), Kazuhiro Tanaka1), Mitsutoshi Nakada2), Naoya Hashimoto3), Manabu Kinoshita3), Naoki Kagawa3), Katsu Mizukawa1), Hirotomo Tanaka1), Takashi Miaowaki1), Hiroaki Nagashima1), Junichi Sakata1), Eiji Kohmura1)
Organizer
Society for Neuro-Oncology Annual Scientific Meeting
Place of Presentation
San Antonio
Year and Date
2015-11-19 – 2015-11-22
Related Report
Int'l Joint Research
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