| Project/Area Number |
25462279
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
Yoshida Daizo 日本医科大学, 医学部, 准教授 (30210701)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA AKIO 日本医科大学, 大学院医学研究科, 教授 (60302725)
TAKUMI ICHIRO 日本医科大学, 医学部, 講師 (60307923)
KIM KYONSONG 日本医科大学, 医学部, 助教 (30339387)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Gremlin / 下垂体腫瘍 / tumor angiogenesis / signal transduction / BMP / pituitary adenoma / signal cascade / molecular morphology / angiogenesis / pitutary adenoma / tissue microarray |
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| Outline of Final Research Achievements |
Expression of Gremlin and CD34 was performed in pituitary adenoma tissues obtained during transsphenoidal surgery in 45 cases (7 PRLoma, 17 GHoma, 17, 2 ACTHoma, 2 TSHoma). CD34-positive vessels coexisted with Gremlin (p<0.005, r2=0.8589), and Gremlin and CD34 colocalized significantly (mean=0.690). Gremlin and microvascular density (MVD) were detected by double-immunofluorescence microscopy in CD34-positive vessels from tissue microarray analysis of 60 cases of pituitary adenomas (6 PRLoma, 23 GHoma, 22 NFoma, 5 ACTHoma, and 4 TSHoma). MVD was significantly correlated with an increased Gremlin level (linear regression: p<0.005 r2=0.4958). In contrast, Gremlin expression showed no correlation with tumor subtype or Knosp score. The high level of expression of Gremlin in pituitary adenoma tissue with many CD34-positive vessels and the strong coherence of these regions indicate that Gremlin is associated with angiogenesis in pituitary adenoma cells.
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