The mechanisms of autophagic cell death induction by histone deacetylase inhibitor SAHA

• Project/Area Number: 25462338
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyushu University
• Principal Investigator: Okada Takamitsu 九州大学, 大学病院, 助教 (70525550)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ヒストン脱アセチル化酵素阻害剤 / オートファジー細胞死 / 多剤耐性骨肉腫細胞株 / HDAC inhibitor / autophagy / cell cycle / autophagic cell death / HDACI / SAHA / multi-drug resistance

Outline of Final Research Achievements

There was no correlation between the expression of autophagy-related protein and acetylation of the histone. I was able to confirm that quantity of population of G2/M increased by SAHA. However, there was no correlation between G2/M introduction time and LC3-2 expression increasing time. SAHA induced the formation of autophagosome in a cell. I observed the period of the formation of autophagosome , there was no tendency of this period. I tried the making of the multidrug-resistant osteosarcoma cell animal model using osteosarcoma cell line: Saos2, but this cell line died the concentration of 25 ng/ml Dox. We could not make the multidrug-resistant Saos2 cell line.

Research Products

• [Journal Article] Effect of the Optimal Asymmetry on the Strength of Six-Strand Tendon Repair: An Ex Vivo Biomechanical Study. (2017)
  • Author(s): Kozono N, Okada T, Takeuchi N, Shimoto T, Higaki H, Nakashima Y
  • Journal Title: J Hand Surg Am. Volume: 42(4) Issue: 4 Pages: 250-256
  • DOI: 10.1016/j.jhsa.2017.01.015
  • Peer Reviewed
• [Journal Article] In vivo kinematic analysis of the glenohumeral joint during dynamic full axial rotation and scapular plane full abduction in healthy shoulders (2016)
  • Author(s): Naoya Kozono, Takamitsu Okada, Naohide Takeuchi, Satoshi Hamai, Hidehiko Higaki, Satoru Ikebe, Takeshi Shimoto, Go Miake, Yoshitaka Nakanishi, Yukihide Iwamoto
  • Journal Title: Knee Surgery, Sports Traumatology, Arthroscopy Volume: - Issue: 7 Pages: 1-9
  • DOI: 10.1007/s00167-016-4263-2
  • Peer Reviewed
• [Presentation] In Vino Kinematic Analysis of the Glenohumeral Joint during Dynamic Full Axial Rotation and Scapular Plane Full Abduction (2017)
  • Author(s): Kozono N, Okada T, Takeuchi N, Shimoto T, Higaki H, Nakashima Y
  • Organizer: The ORS 2017 Annual Meeting
  • Place of Presentation: San Diego, California
  • Year and Date: 2017-03-19
  • Int'l Joint Research