| Project/Area Number |
25462356
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | National Hospital Organization, Kyushu Cancer Center |
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Principal Investigator |
Takiguchi Soichi 独立行政法人国立病院機構(九州がんセンター臨床研究センター), その他部局等, 研究員 (00280793)
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| Co-Investigator(Kenkyū-buntansha) |
IGUCHI Haruo 四国がんセンター, 臨床研究センター, 顧問 (90393438)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUSUE Kimihiko 福岡大学, 薬学部, 准教授 (10389364)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 骨転移 / ケモカイン / 走化性 / 足場非依存性増殖 / 癌転移関連因子 / 骨芽細胞 / 破骨細胞 |
|---|
| Outline of Final Research Achievements |
To investigate the molecular mechanisms of lung cancer-induced bone metastasis, we established a bone-seeking subclone (HARA-B4) from a human squamous lung cancer cell line (HARA) using an in vivo selection method. From the comparison of comprehensive gene expression profiles between HARA and HARA-B4, we identified CXC chemokine ligand 14 (CXCL14) as a critical factor for the formation of bone metastasis. CXCL14 was expressed 5-fold greater in HARA-B4 than in HARA, and shown to be involved in the anchorage-independent growth of cancer cells, and enhancement of cancer cell tropism to the bone possibly by the recruitment of bone marrow cells to cancer cells. Furthermore, in clinical specimens of lung cancer-induced bone metastasis, expression of CXCL14 was observed in the tumor cells infiltrated in bone marrow in all specimens examined.
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