Myocardial preconditioning effects of high-dose insulin and PI3K/Akt signaling pathway

• Project/Area Number: 25462429
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: University of Yamanashi
• Principal Investigator: OGUCHI Takeshi 山梨大学, 総合研究部, 准教授 (60201399)
• Co-Investigator(Renkei-kenkyūsha): MATSUKAWA Takashi 山梨大学, 総合研究部, 教授 (80209519) ; ISHIYAMA Tadahiko 山梨大学, 医学部附属病院, 准教授 (90293448)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: インスリン / プレコンディショニング / 高血糖 / 虚血再灌流障害

Outline of Final Research Achievements

Acute hyperglycemia diminishes the cardioprotection induced by ischemic preconditioning in the isolated rat heart. The current study showed that acute hyperglycemia reduced the p-Akt level, in spite of insulin administration prior to the ischemic period. Inhibition of myocardial Akt phosphorylation is a possible mechanism by which hyperglycemia attenuates the cardioprotective effect of insulin. Normalizing blood glucose before ischemia is important to maximize insulin-induced cardioprotection in stunned rat heart.