Identification of AKT3-HIF1å pathway as a new therapeutic target for renal cell carcinoma

• Project/Area Number: 25462495
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Yokohama City University
• Principal Investigator: KONDO Keiichi 横浜市立大学, 附属病院, 准教授 (80363836)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: AKT / HIFα / 腎細胞癌 / AKT3 / AKTサブタイプ / HIFαサブタイプ / HIF1α

Outline of Final Research Achievements

We started this study from suppression experiment with renal carcinoma cell line plus siRNA for suppression of hypoxia inducible factor alpha subunit, to expression analysis of each subtype of AKT and HIF alpha subunit in surgical specimens of renal cell carcinomas and statistical analysis of relationship between expression level of these subtypes and pathological factors & prognosis.
Suppression of each AKT subtype show suppression of HIF alpha subtype, but combination of AKT subtype suppression and HIF alpha subtype suppression were different among renal carcinoma cell lines. Original histopathological factors of renal carcinoma cell line and status of von Hippel-Lindau tumor suppressor gene influenced these differences.
Expression of AKT subtype couldn't show relationship with the prognosis of renal cell carcinoma patients.

Research Products

• [Journal Article] Prognostic value of the bone scan index using a computer-aided diagnosis system for bone scans in hormone-naive prostate cancer patients with bone metastases. (2016)
  • Author(s): Miyoshi Y, Yoneyama S, Kawahara T, Hattori Y, Teranishi J, Kondo K, Moriyama M, Takebayashi S, Yokomizo Y, Yao M, Uemura H, Noguchi K.
  • Journal Title: BMC Cancer Volume: 16 Issue: 1 Pages: 128-128
  • DOI: 10.1186/s12885-016-2176-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prognostic value of a computer-aided diagnosis system involving bone scans among men treated with docetaxel for metastatic castration-resistant prostate cancer. (2016)
  • Author(s): Uemura K, Miyoshi Y, Kawahara T, Yoneyama S, Hattori Y, Teranishi J, Kondo K, Moriyama M, Takebayashi S, Yokomizo Y, Yao M, Uemura H, Noguchi K.
  • Journal Title: BMC Cancer Volume: 16 Issue: 1 Pages: 109-109
  • DOI: 10.1186/s12885-016-2160-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] FDG PET/CT as a prognostic biomarker in the era of molecular-targeting therapies: max SUVmax predicts survival of patients with advanced renal cell carcinoma. (2016)
  • Author(s): Nakaigawa N, Kondo K, Tateishi U, Minamimoto R, Kaneta T, Namura K, Ueno D, Kobayashi K, Kishida T, Ikeda I, Hasumi H, Makiyama K, Kubota Y, Inoue T, Yao M.
  • Journal Title: BMC Cancer Volume: 16 Issue: 1 Pages: 67-67
  • DOI: 10.1186/s12885-016-2097-4
  • Peer Reviewed / Open Access
• [Journal Article] Tumor suppressive function of PTPN23 in testicular germ cell tumors is lost upon overexpression of miR-142-3p (2013)
  • Author(s): Kiyoko Tanaka
  • Journal Title: Journal of Biological Chemistry Volume: - Issue: 33 Pages: 23990-23999
  • DOI: 10.1074/jbc.m113.478891
  • Peer Reviewed
• [Presentation] 腎細胞癌におけるHIFαサブタイプとAKTサブタイプの相関性の定量的解析 (2017)
  • Author(s): 近藤 慶一
  • Organizer: 第26回泌尿器科分子・細胞研究会
  • Place of Presentation: 全労済ソレイユ（大分県大分市）
  • Year and Date: 2017-03-11
• [Presentation] 腎細胞癌におけるHIFαサブタイプとAKTサブタイプの関連性 (2016)
  • Author(s): 近藤 慶一
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-07