Identification of the function of legumain in prostate cancer

• Project/Area Number: 25462501
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Tokyo Medical University
• Principal Investigator: Ohno Yoshio 東京医科大学, 医学部, 准教授 (40266482)
• Co-Investigator(Kenkyū-buntansha): 中島 淳 東京医科大学, 医学部, 教授 (10167546) ; 橘 政昭 東京医科大学, 医学部, 兼任助教 (70129526)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 前立腺がん / レグメイン / 浸潤 / 低酸素 / 前立腺癌 / 骨転移

Outline of Final Research Achievements

Legumain is highly expressed in prostate cancer and other solid tumors and is a potential anticancer target. Here we investigated the role of legumain in invasion and metastasis in prostate cancer cell lines. In vitro invasion assay did not show significant difference in invasive ability regardless of legumain expression level.　In addition, hypoxic condition did not change legumain mRNA expression in prostate cancer cells. Therefore, we need experiment under chronic hypoxic condition and established hypoxia resistant prostate cancer cell line by continuous cultivation over 6 months. Legumain expression on mRNA level in hypoxia resistant cell lines was higher than that in parental cell lines. We have a plan of further study.

Research Products

• [Journal Article] Development of a Nomogram for Predicting Severe Neutropenia Associated With Docetaxel-Based Chemotherapy in Patients With Castration-Resistant Prostate Cancer. (2017)
  • Author(s): Hirasawa Y, Nakashima J, Sugihara T, Takizawa I, Gondo T, Nakagami Y, Horiguchi Y, Ohno Y, Namiki K, Ohori M, Tachibana M.
  • Journal Title: Clinical Genitourinary Cancer Volume: 15 Issue: 1 Pages: 176-181
  • DOI: 10.1016/j.clgc.2016.05.012
  • Peer Reviewed
• [Journal Article] Association between preoperative serum total cholesterol level and biochemical recurrence in prostate cancer patients who underwent radical prostatectomy (2016)
  • Author(s): Ohno Y, Ohori M, Nakashima J, Okubo H, Satake N, Hashimoto T, Tachibana M
  • Journal Title: Molecular and clinical oncology Volume: in press Issue: 6 Pages: 1073-1077
  • DOI: 10.3892/mco.2016.831
  • Peer Reviewed
• [Journal Article] Preoperative predictive factors and further risk stratification of biochemical recurrence in clinically localized high-risk prostate cancer. (2016)
  • Author(s): Hamada R, Nakashima J, Ohori M, Ohno Y, Komori O, Yoshioka K, Tachibana M
  • Journal Title: International Journal of Clinical Oncology Volume: in press
  • Peer Reviewed
• [Journal Article] Impact of a preoperatively estimated prostate volume using transrectal ultrasonography on surgical and oncological outcomes in a single surgeon's experience with robot-assisted radical prostatectomy. (2016)
  • Author(s): Hirasawa Y, Ohno Y, Nakashima J, Shimodaira K, Hashimoto T, Gondo T, Ohori M, Tachibana M, Yoshioka K
  • Journal Title: Surgical Endoscopy Volume: in press
  • Peer Reviewed
• [Journal Article] Association of legumain expression pattern with prostate cancer invasiveness and aggressiveness (2013)
  • Author(s): Ohno Y1, Nakashima J, Izumi M, Ohori M, Hashimoto T, Tachibana M.
  • Journal Title: World J Urol Volume: 31 Issue: 2 Pages: 359-364
  • DOI: 10.1007/s00345-012-0977-z
  • Peer Reviewed