Elucidation of the inhibiting mechanism of the urolithiasis using the antioxidant effect of the female hormone and the clinical application

• Project/Area Number: 25462523
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: Hirose Masahito 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70529172)
• Co-Investigator(Kenkyū-buntansha): KOHRI Kenjiro 名古屋市立大学, 学長 (30122047) ; YASUI Takahiro 名古屋市立大学, 大学院医学研究科, 教授 (40326153) ; OKADA Atsushi 名古屋市立大学, 大学院医学研究科, 講師 (70444966) ; NIIMI Kazuhiro 名古屋市立大学, 大学院医学研究科, 研究員 (70551274)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 尿路結石 / 女性ホルモン / 酸化ストレス / 細胞障害 / ミトコンドリア / オステオポンチン

Outline of Final Research Achievements

In this study, we examined about sex differences in period of stone formation, for the elucidation about stone formation mechanism and the reason that the woman urolithiasis patient is few. The result is following three points. ① We clarified a stone formation process at an ultrastructural level. ② We clarified stone formation inhibiting effect of the female hormone. ③ We elucidated the relationship of female hormone and the stone-related gene: osteopontin. In addition, the possibility of the new calculus prevention using female hormone was thought by the result of this study.

Research Products

• [Presentation] The effect of green tea for the early period of renal crystal formation (2016)
  • Author(s): 広瀬 真仁、安井 孝周、岡田 淳志、郡 健二郎
  • Organizer: 13th International Symposium on Urolithiasis
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2016-07-19
  • Int'l Joint Research
• [Presentation] The role of renal tubular cell injury in the earlyperiod of renal crystal formation identified from the cell injury-inhibiting effect of green tea. (2015)
  • Author(s): 広瀬 真仁、安井 孝周、岡田 淳志、郡 健二郎
  • Organizer: American Urological Association Annual Meeting 2015
  • Place of Presentation: New Orleans（USA）
  • Year and Date: 2015-05-16
  • Int'l Joint Research
• [Presentation] N-methyl-4-isoleucine cyclosporine, an inhibitor of mitochondrial cyclophilin D activation, prevents kidney stone formation by alleviating oxidative stress in renal tubular cells. (2015)
  • Author(s): 安井 孝周、広瀬 真仁、新美 和寛、岡田 淳志、郡 健二郎
  • Organizer: American Urological Association Annual Meeting 2015
  • Place of Presentation: New Orleans（USA）
  • Year and Date: 2015-05-16
  • Int'l Joint Research