Inner cell mass division during human blastcyst formation in vitro culture system.

• Project/Area Number: 25462549
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Akita University
• Principal Investigator: Terada Yukihiro 秋田大学, 医学(系)研究科(研究院), 教授 (10260431)
• Co-Investigator(Kenkyū-buntansha): KABASHIMA Katsuya 秋田大学, 医学系研究科, 技術系補佐員 (30615422) ; KUMAGAI Jin 秋田大学, 医学系研究科, 准教授 (60333936) ; KUMAZAWA Yukiyo 秋田大学, 医学系研究科, 助教 (70400504) ; 舘山 奈江 秋田大学, 医学部, 技術系補佐員 (20638283)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ヒト胚 / 胚発育 / 胚内部細胞塊分裂 / 胚凍結 / 胚盤胞

Outline of Final Research Achievements

This study is an examination about the unnatural separation of the inner cell mass cells during human in vitro blastocyst formation.　This phenomenon was found by continuous observation by timelapse cinematography in the in vitro culture system of the mammalian embryo. This behavior was frequently observed in embryos after the freeze thaw (JRD,2016), also frequently observed under condition with the functional inhibitor of the gap junction (JARG,2015). Furthermore, the embryo which this behavior was found was thought to have low the developmental potential. These findings suggested that human embryo freeze thaw have influence on embryonic development behavior and the quality of embryos. The new scientific indicator of the embryo evaluation will be developed by the continuous observation/evaluation of the embryonic development.

Research Products

• [Journal Article] Time-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos (2016)
  • Author(s): Shimoda Y,Kumagai J, Anzai M,Kabashima K,Togashi K,Miura Y,Shirasawa H,Sato W,Kumazawa Y, Terada Y
  • Journal Title: Journal of Reproduction and Development Volume: 62 Issue: 2 Pages: 187-193
  • DOI: 10.1262/jrd.2015-150
  • NAID: 130005146468
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets. (2015)
  • Author(s): Shirasawa H,Kumagai J, Sato E, Kabashima K,Kumazawa Y,Sato W,Miura H,Nakamura R,Nanjo H, Minamiya Y,Akagami Y,Terada Y
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 15371-15371
  • DOI: 10.1038/srep15371
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 40歳以上の体外受精による妊娠症例の周産期予後に関する検討 (2015)
  • Author(s): 富樫嘉津恵、熊谷仁、白澤弘光、佐藤亘、熊澤由紀代、金森恭子、佐藤朗、兒玉英也、寺田幸弘
  • Journal Title: 秋田県産科婦人科 Volume: 20 Pages: 3-7
  • NAID: 40020738082
  • Peer Reviewed
• [Journal Article] Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometorial carcinoma: a novel strategy for human oocyte research (2013)
  • Author(s): Shirasawa, H., Kumagai, J., Sato, W., Kumazawa, Y., Sato, N., Terada, Y
  • Journal Title: J Assist Reprod Genet Volume: 30（9） Pages: 1227-30
  • Peer Reviewed
• [Journal Article] Repeated recombinant activated factor VII administration in a patient with congenital factor VII deficiency undergoing modified radical hysterectomy: a case report. (2013)
  • Author(s): Shirasawa, H., Yoshioka, T., Sawada, K., Terada, Y
  • Journal Title: Haemophilia Volume: 20（1） Issue: 1
  • DOI: 10.1111/hae.12312
  • Peer Reviewed
• [Presentation] ギャップジャンクション阻害剤存在下では 胞胚腔の虚脱が増加する． (2015)
  • Author(s): 富樫嘉津恵、佐藤恵美子、椛嶋克哉、下田勇輝、白澤弘光、佐藤亘、熊澤由紀代、熊谷仁、兒玉英也、寺田幸弘
  • Organizer: 第56回日本卵子学会
  • Place of Presentation: 栃木
  • Year and Date: 2015-05-30
• [Presentation] ヒト卵胞液の大学病院間輸送方法および輸送後の体外成熟培養結果に関する検討． (2015)
  • Author(s): 白澤弘光、佐藤恵美子、熊谷仁、富樫嘉津恵、下田勇輝、佐藤亘、熊澤由紀代、井原基公、宇都宮裕貴 八重樫伸生 寺田幸弘
  • Organizer: 第56回日本卵子学会
  • Place of Presentation: 栃木
  • Year and Date: 2015-05-30
• [Presentation] Novel rapid immunofluorescence staining method for the murine egg using the non-contact alternating current electric field mixing of the microdroplet. (2015)
  • Author(s): Shirasawa H,Kumagai J,Sato E, Kumazawa Y,Sato W,Togashi K,Terada Y
  • Organizer: International Federation of Fertility Societies/Japan Society for Reproduction Medicine International Meeting
  • Place of Presentation: 横浜
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] Gap junction inhibitor induced aberrant inner cell mass behavior in pre-implantation mouse embryos. (2015)
  • Author(s): Togashi K,Kumagai J,Saot E,Shirasawa H,Sato W, Kanamori K,Kumazawa Y,Kabashima K,Kodama H, Omori Y, Terada Y
  • Organizer: International Federation of Fertility Societies/Japan Society for Reproduction Medicine International Meeting
  • Place of Presentation: 横浜
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] ギャップジャンクションの機能阻害はマウス胚で‘strand現象’を惹起する． (2015)
  • Author(s): 富樫嘉津恵、白澤弘光、佐藤亘、金森恭子、熊澤由紀代、熊谷仁、兒玉英也、寺田幸弘
  • Organizer: 第67回日本産科婦人科学会
  • Place of Presentation: 横浜
  • Year and Date: 2015-04-10
• [Presentation] 卵胞液の施設間輸送方法の改善が摘出卵巣由来のヒト未成熟卵子に対する体外成熟培養結果へ与える影響に関する検討． (2015)
  • Author(s): 白澤弘光 熊谷仁 富樫嘉津恵、佐藤亘、熊澤由紀代、井原基公、宇都宮裕貴、八重樫伸生、寺田幸弘
  • Organizer: 第67回日本産科婦人科学会
  • Place of Presentation: 横浜
  • Year and Date: 2015-04-10
• [Book] 産婦人科の実際 (2015)
  • Author(s): 志賀尚美、宇都宮裕貴、黒澤大樹、菅沼亮太、福井淳史、高橋俊文、阿部宏之、寺田幸弘、八重樫伸生
  • Total Pages: 123
  • Publisher: 金原出版株式会社
• [Book] 今日の治療方針 (2015)
  • Author(s): 佐藤亘
  • Total Pages: 2096
  • Publisher: 医学書院