Analysis of the genes which are involved in a spermatogenic defect in higher temperature in mouse

• Project/Area Number: 25462572
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka City University
• Principal Investigator: YOSHIDA Kayo 大阪市立大学, 大学院医学研究科, 准教授 (30311921)
• Co-Investigator(Kenkyū-buntansha): KIZU Akane 大阪市立大学, 大学院医学研究科, 助教 (30623201)
• Co-Investigator(Renkei-kenkyūsha): KOYAMA Masayasu 大阪市立大学, 大学院医学研究科, 教授 (00183351)
• Research Collaborator: MORITA Takashi 大阪市立大学, 大学院医学研究科, 教授 (70150349) ; KITADA Kohei 大阪市立大学, 大学院医学研究科, 大学院生 ; HAYASHI Masami 大阪市立大学, 大学院医学研究科, 大学院生
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生殖医学 / 精原細胞 / 精子形成 / 停留精巣

Outline of Final Research Achievements

A low birthrate is an important problem in Japan, and the influence by sterility is also big problem. Decrease of the number of male spermatozoa and increase of abnormal sperm are reported in recent years. A testicle is included in the scrotum whose temperature is lower about 3 ℃ than the body temperature in the mammals. There must be a mechanism that cannot move forward spermatogenesis by higher temperature. We kept mouse testes in higher temperature. It made the mouse testes to stop spermatogenesis as judged by histological analyses. In order to identify genes that will be involved in spermatogenesis, we collected RNAs from mouse testes at higher temperature and examined its expression using DNA chips. We can selected several genes whose expression were increased or decrease by higher temperature. We will reveal the physiological significance of the genes by making mice lacking such genes in spermatogenesis.

Research Products

• [Journal Article] Gene-modified embryonic stem cell test to characterize chemical risks. (2015)
  • Author(s): Kitada K, Kizu A, Teramura T, Takehara T, Hayashi M, Tachibana D, Wanibuchi H, Fukushima S, Koyama M, Yoshida K, Morita T.
  • Journal Title: Environ Sci Pollut Res Int. Volume: 22 Issue: 22 Pages: 18252-18259
  • DOI: 10.1007/s11356-015-5051-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Estimation of effects of space radiation using frozen mouse ES cells in ISS (2014)
  • Author(s): K.YOSHIDA, M. HADA, K. EGUCHI-KASAI, T.TERAMURA, F. A. CUCINOTTA and T. MORITA
  • Journal Title: Journal of Radiation Research Volume: ５５ Issue: suppl 1 Pages: i12-i13
  • DOI: 10.1093/jrr/rrt217
  • Peer Reviewed
• [Presentation] Detection of Chromosome Aberration by using Histone H2AX-deficient mouse ES cells (2013)
  • Author(s): K.Yoshida, M. Hada, K. Eguchi-Kasai, T.Teramura, F. A. Cucinotta and T. Morita
  • Organizer: Heavy Ion in Therapy and Space Radiation Symposium 2013
  • Place of Presentation: 京葉銀行文化プラザ