| Project/Area Number |
25462864
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
FUJITA Shuichi 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (00181355)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMADA Shin-ichi 信州大学, 大学院医学系研究科, 准教授 (50380853)
IKEDA Tohru 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00211029)
SHIBATA Yasuaki 長崎大学, 医歯薬学総合研究科(医学系), 講師 (80253673)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 微小環境 / 扁平上皮癌 / リンパ節転移 / 癌関連マクロファージ / 癌関連好中球 / 癌関連線維芽細胞 / CCL2 / CCR2 / marginal histiocytosis / CAF / 口腔扁平上皮癌 / 転移 / 腫瘍関連好中球 / 腫瘍関連マクロファージ / CCL2-CCR2 pathway / 癌幹細胞 / CD204 |
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| Outline of Final Research Achievements |
Oral squamous cell carcinoma (SCC) often metastasizes to cervical lymph nodes. We immunohistochemically examined the involvement of CCL2-CCR2 axis with construction of microenvironment suitable for metastasis in lymph nodes.Marginal sinus histiocytosis was frequently observed in metastatic cases. CCL2 was expressed in tumor-associated neutrophils (TANs) and moderately or poorly differentiated SCC was detected at primary tumor sites. TANs expressing CCL2 flowed into the marginal sinus in the lymph nodes. CCR2-positive macrophages and mesenchymal cells infiltrated the tumor stroma and were seen within the carcinoma nests. They were predominantly present in the marginal sinus of metastatic cases. In small metastatic foci, alpha-SMA-positive spindle cells resembling carcinoma-associated fibroblasts (CAFs) were observed adjacent to the macrophages. The results indicate that the CCL2-CCR2 axis is associated with lymphatic metastasis from oral squamous cell carcinoma.
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