| Project/Area Number |
25462875
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
Irie Tarou 昭和大学, 歯学部, 講師 (00317570)
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| Co-Investigator(Kenkyū-buntansha) |
MISHIMA Kenji 昭和大学, 歯学部, 教授 (50275343)
MORI Taisuke 国立研究開発法人国立がん研究センター, 分子病理分野, 研究員 (00296708)
YASUHARA Rika 昭和大学, 歯学部, 助教 (20453649)
YAMAMOTO Go 昭和大学, 歯学部, 兼任講師 (80384189)
外薗 知恵 昭和大学, 歯学部, 助教 (10555296)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUJI Takashi 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (50339131)
TOYOSHIMA Koh-ei 北里大学, 医学部, 特任講師 (00599243)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 唾液腺腫瘍 / モデルマウス / 腫瘍組織発生 / コンディショナルマウス / 腫瘍モデルマウス / コンディショナルトランスジェニックマウス |
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| Outline of Final Research Achievements |
Details of histogenesis of salivary gland tumors are still not entirely clear. We performed challenge for establishment of a Cre-conditional Plag1-driven salivary gland tumor mouse model which causes PLAG1 overexpression only in basal, myoepithelial or luminal cells of salivary gland. Two independent lines of Plag1 overexpressed (CAG-loxp-lacZ-pA-loxp-Plag1-EGFP) flox mouse were established. Two kinds of conditional mouse which overexpressed PLAG1 only in luminal cells (Sox9-CreERT2-loxP mouse) or myoepithelial cells (Myh11-Cre-loxP mouse) of salivary glands are available at the present time. Moreover, we established three stable PLAG1 overexpressed cell lines of acinar-, duct- and myoepithelial phenotype of human normal salivary glands. Our results by use of these stable cell lines indicated that PLAG1 may play a dual role in salivary gland tumorigenesis in cell specific manner.
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