Establishment of a Cre-conditional Plag1-driven salivary gland tumor mouse model.
| Project/Area Number |
25462875
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
Irie Tarou 昭和大学, 歯学部, 講師 (00317570)
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| Co-Investigator(Kenkyū-buntansha) |
MISHIMA Kenji 昭和大学, 歯学部, 教授 (50275343)
MORI Taisuke 国立研究開発法人国立がん研究センター, 分子病理分野, 研究員 (00296708)
YASUHARA Rika 昭和大学, 歯学部, 助教 (20453649)
YAMAMOTO Go 昭和大学, 歯学部, 兼任講師 (80384189)
外薗 知恵 昭和大学, 歯学部, 助教 (10555296)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUJI Takashi 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (50339131)
TOYOSHIMA Koh-ei 北里大学, 医学部, 特任講師 (00599243)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 唾液腺腫瘍 / モデルマウス / 腫瘍組織発生 / コンディショナルマウス / 腫瘍モデルマウス / コンディショナルトランスジェニックマウス |
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| Outline of Final Research Achievements |
Details of histogenesis of salivary gland tumors are still not entirely clear. We performed challenge for establishment of a Cre-conditional Plag1-driven salivary gland tumor mouse model which causes PLAG1 overexpression only in basal, myoepithelial or luminal cells of salivary gland. Two independent lines of Plag1 overexpressed (CAG-loxp-lacZ-pA-loxp-Plag1-EGFP) flox mouse were established. Two kinds of conditional mouse which overexpressed PLAG1 only in luminal cells (Sox9-CreERT2-loxP mouse) or myoepithelial cells (Myh11-Cre-loxP mouse) of salivary glands are available at the present time. Moreover, we established three stable PLAG1 overexpressed cell lines of acinar-, duct- and myoepithelial phenotype of human normal salivary glands. Our results by use of these stable cell lines indicated that PLAG1 may play a dual role in salivary gland tumorigenesis in cell specific manner.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Zinc transporter SLC39A10/ZIP10 controls humoral immunity by modulating B-cell receptor signal strength.2014
Author(s)
Hojyo S., T. Miyai, H. Fujishiro, M. Kawamura, T. Yasuda, A. Hijikata, B-H Bin, T. Irie, J. Tanaka, T. Atsumi, M. Murakami, M. Nakayama, O. Ohara, S. Himeno, H. Yoshida, H. Koseki, T. Ikawa, K. Mishima, and T. Fukada.
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Journal Title
Proc Natl Acad Sci USA
Volume: 111
Issue: 32
Pages: 11786-11791
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Zinc transporter SLC39A10/ZIP10 facilitates antiapoptotic signaling during early B-cell development2014
Author(s)
Miyai T, Hojyo S, Ikawa T, Kawamura M, Irie T, Ogura H, Hijikata A, Bin BH, Yasuda T, Kitamura H, Nakayama M, Ohara O, Yoshida H, Koseki H, Mishima K, Fukada T
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 111
Issue: 32
Pages: 11780-11785
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma.2013
Author(s)
Hayashi S, Tanaka J, Okada S, Isobe T, Yamamoto G, Yasuhara R, Irie T, Akiyama C, Kohno Y, Tachikawa T, Mishima K.
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Journal Title
Exp Cell Res
Volume: 319
Issue: 8
Pages: 1220-1228
DOI
Related Report
Peer Reviewed
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