• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Screening of novel inhibitors to hydrogen sulfide-producing enzymes -based on enzyme structures and reaction mechanisms- that can prevent oral malodor

Research Project

Project/Area Number 25462897
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionIwate Medical University

Principal Investigator

KEZUKA Yuichiro  岩手医科大学, 薬学部, 助教 (50397163)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Yasuo  愛知学院大学, 歯学部, 准教授 (10315096)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords硫化水素産生酵素 / 酵素 / 歯周病 / 口臭 / 化合物スクリーニング / 反応中間体 / 硫化水素産生
Outline of Final Research Achievements

Structral and enzymatic analyses of hydrogen sulfide-producing enzymes from a periodontal pathogen, Fusobacterium nucleatum, were performed. We identified four important amino acid residues on catalysis and/or substrate (reaction intermediate) binding, and revealed their roles and contributions to the catalytic activity. On the basis of these results, the reaction mechanism of hydrogen sulfide-producing enzyme Fn1055 was proposed. In parallel, we constructed two assay systems for inhibitor screening. Using the assay systems, small molecules from chemical libraries were screened.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (13 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Acyl-CoA reductase PGN_0723 utilizes succinyl-CoA to generate succinate semialdehyde in a butyrate-producing pathway of Porphyromonas gingivalis2016

    • Author(s)
      1. Yoshida, Y., Sato, M., Kezuka, Y., Hasegawa, Y., Nagano, K., Takebe, J. and Yoshimura, F.
    • Journal Title

      Arch. Biochem. Biophys.

      Volume: 596 Pages: 138-148

    • DOI

      10.1016/j.abb.2016.03.014

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crystal structures of βC-S lyase from Streptococcus anginosus in complex with its reaction intermediates2013

    • Author(s)
      Yuichiro Kezuka
    • Journal Title

      Photon Factory Activity Report

      Volume: 30 Pages: 275-275

    • Related Report
      2013 Research-status Report
  • [Presentation] Fusobacterium nucleatum由来硫化水素産生酵素の立体構造と反応機構2016

    • Author(s)
      毛塚雄一郎、吉田康夫、野中孝昌
    • Organizer
      2015年度量子ビームサイエンスフェスタ
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2016-03-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 歯周病原細菌由来硫化水素産生酵素の構造と反応機構2015

    • Author(s)
      毛塚雄一郎、吉田康夫、野中孝昌
    • Organizer
      第54回日本薬学会東北支部大会
    • Place of Presentation
      岩手医科大学矢巾キャンパス(紫波郡矢巾町)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 歯周病原細菌メチオニン γ-リアーゼの活性測定と結晶化の検討2015

    • Author(s)
      鈴木喬介、佐藤裕也、近野平、遠藤彩姫、毛塚雄一郎、野中孝昌
    • Organizer
      第54回日本薬学会東北支部大会
    • Place of Presentation
      岩手医科大学矢巾キャンパス(紫波郡矢巾町)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] グルタチオン S-トランスフェラーゼ及びヒスチジンタグを融合したタンパク質の発現と精製2015

    • Author(s)
      藤原茂晃、福谷俊治、植田光彦、毛塚雄一郎、野中孝昌
    • Organizer
      第54回日本薬学会東北支部大会
    • Place of Presentation
      岩手医科大学矢巾キャンパス(紫波郡矢巾町)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] モジュラーキチナーゼの立体構造と抗真菌活性の相関解明への試み2015

    • Author(s)
      毛塚雄一郎、関安孝、野中孝昌
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 歯周病原細菌メチオニンγ-リアーゼの活性測定と結晶化の検討2014

    • Author(s)
      鈴木喬介、佐藤裕也、近野平、遠藤彩姫、毛塚雄一郎、野中孝昌
    • Organizer
      第53回日本薬学会東北支部大会
    • Place of Presentation
      いわき明星大学(福島県いわき市)
    • Year and Date
      2014-10-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯周病原細菌由来硫化水素産生酵素の構造と反応機構2014

    • Author(s)
      毛塚雄一郎、吉田康夫、野中孝昌
    • Organizer
      第14回日本蛋白質科学会
    • Place of Presentation
      横浜産貿ホールマリネリア(神奈川県横浜市)
    • Year and Date
      2014-06-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Treponema denticolaメチオニンγ-リアーゼの酵素活性測定と結晶化の検討2014

    • Author(s)
      佐藤裕也、毛塚雄一郎、野中孝昌
    • Organizer
      日本生化学会東北支部 第80回例会
    • Place of Presentation
      アキタパークホテル(秋田県秋田市)
    • Year and Date
      2014-05-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯周病原細菌由来硫化水素産生酵素の構造と反応機構

    • Author(s)
      毛塚雄一郎
    • Organizer
      第13回日本蛋白質科学会
    • Place of Presentation
      鳥取市、とりぎん文化会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 口腔細菌由来硫化水素産生酵素βC-S lyaseの構造と反応機構

    • Author(s)
      野中孝昌
    • Organizer
      第24回 フォーラム・イン・ドージン
    • Place of Presentation
      熊本市、熊本ホテルキャッスル
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 口腔細菌由来硫化水素産生酵素βC-S lyaseの反応中間体の結晶構造解析

    • Author(s)
      野中孝昌
    • Organizer
      平成25年度日本薬学会東北支部 第35回東北薬学セミナー
    • Place of Presentation
      仙台市、東北薬科大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 歯周病原細菌由来硫化水素産生酵素の構造と反応機構

    • Author(s)
      毛塚雄一郎
    • Organizer
      第14回日本蛋白質科学会
    • Place of Presentation
      横浜市、ワークピア横浜・横浜産貿ホールマリネリア
    • Related Report
      2013 Research-status Report
  • [Presentation] Structure of hydrogen sulfide-producing enzyme from a periodontal pathogen

    • Author(s)
      Yuichiro Kezuka
    • Organizer
      23rd Congress and General Assembly of the International Union of Crystallography
    • Place of Presentation
      モントリオール
    • Related Report
      2013 Research-status Report
  • [Remarks] 和漢薬の科学基盤形成拠点

    • URL

      http://www.inm.u-toyama.ac.jp/jp/collabo/idx_collabo.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi