| Project/Area Number |
25462900
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHINO Hiromi 東京歯科大学, 歯学部, 助教 (00445446)
ONODERA Shoko 東京歯科大学, 歯学部, 助教 (90637662)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | iPS細胞 / 骨代謝 / 骨芽細胞 / 再生医療 / 疾患特異的iPS細胞 / 骨細胞 / Wnt / ヘッジホッグ / 骨再生 / 骨リモデリング / 骨欠損モデル / アルカリフォスファターゼ / 骨組織 |
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| Outline of Final Research Achievements |
We developed new strategies to generate all stages of osteogenic cells including mesenchymal stem cells, pre-osteoblasts, osteoblast and osteocytes. These cells are expressed their specific marker proteins. We found that PI3K-Akt signaling is essential for early stage of osteogenesis. IGF-1, which is an effective stimulator of PI3K-Akt signaling is a potent inducer of osteogenesis. We found that IGF-1 treatment significantly improve osteogenesis. We established an effective protocol for generating osteocyte from iPS cells. We utilized this protocol to develop new strategy for osteo-regeneration therapy. We transplanted osteoblasts derived from human iPS cells to bone defects generated in Rat and found successful regeneration.We found that ALP is a specific and efficient surface marker to isolate osteolineage cells derved from iPS cells. We established effective protocol to generate osteoblast from human patient derived iPS cells.
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