| Project/Area Number |
25462914
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tohoku University |
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Principal Investigator |
MUROI AZUSA 東北大学, 歯学研究科, 助教 (90463778)
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| Co-Investigator(Kenkyū-buntansha) |
笹野 高嗣 東北大学, 歯学研究科, 教授 (10125560)
菅原 俊二 東北大学, 歯学研究科, 教授 (10241639)
伊藤 あゆみ 東北大学, 大学病院, 医員 (50622990)
西岡 貴志 東北大学, 歯学研究科, 助教 (50641875)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | IL-18 / シェーグレン症候群 / 唾液腺 / インフラマソーム / 唾液腺細胞 |
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| Outline of Final Research Achievements |
Sjogren’s syndrome is an inflammatory autoimmune disease, predominantly characterized by lymphocytic infiltration of the exocrine glands. In this study, to establish prophylactic and therapeutic strategies for this disorder, we examined the association between interleukin 18 (IL-18) and the apoptosis pathway in the salivary gland tissues of humans.
The HSG cell line expresses caspase-1, which processes the IL-18 precursor protein into its mature form. However, ASC and NLRP3, which together with caspase-1 constitute the imflammasome complex, were not detected in these cells. Furthermore, we detected no change in the activity of intracellular caspase-1, even when the cells were stimulated with ATP and nigericin to induce intracellular K+, a factor known to trigger the imflammasome. Therefore, we infer that the activation of IL-18 in this cell line occurs without the activation of the imflammasome.
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