Evaluation of intracanal biota using metagenomics - a new strategy for endodontic treatment
Project/Area Number |
25462945
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Conservative dentistry
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Research Institution | Tohoku University |
Principal Investigator |
YAMAKI KEIKO 東北大学, 歯学研究科(研究院), 助教 (90182419)
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Co-Investigator(Kenkyū-buntansha) |
SATO TAKUICHI 東北大学, 大学院歯学研究科, 講師 (10303132)
MAYANAGI GEN 東北大学, 大学院歯学研究科, 助教 (10451600)
SHIMAUCHI HIDETOSHI 東北大学, 大学院歯学研究科, 名誉教授 (70187425)
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 歯内治療学 / 口腔細菌 / 根尖性歯周炎 / PCR法 / 16S rRNA / 嫌気性菌 / メタゲノム解析 / 根管内細菌叢 / 16s ribosomal RNA / PCR / 感染根管 / 16S ribosomal RNA |
Outline of Final Research Achievements |
Intracanal microflora associated with apical periodontitis was investigated. Under informed consent of patients, dentin samples were collected and analyzed using two rapid microbial cell counters, anaerobic culture, and PCR targeting 16S rRNA genes. Bacterial species found from endodontically suffering canals showed a great diversity in both quality and quantity. Mechanical enlargement of the root canal combined with chemical disinfection could effectively reduce the pre-dominantly anaerobic pathogen. Propionibacterium sp. and Olsenella sp. were frequently recovered in contrast to previous reports. Evaluating the difference in bacterial profile between oral cavity and root canal through metagenomics could lead to a new strategy for diagnosis and treatment of apical periodontitis.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] <i>Porphyromonas bronchialis</i> sp. nov. Isolated from Intraoperative Bronchial Fluids of a Patient with Non-Small Cell Lung Cancer2015
Author(s)
T. Sato, J. Tomida, T. Naka, N. Fujiwara, A. Hasegawa, Y. Hoshikawa, J. Matsuyama, N. Ishida, T. Kondo, K. Tanaka, N. Takahashi, Y. Kawamura
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Journal Title
The Tohoku Journal of Experimental Medicine
Volume: 237
Issue: 1
Pages: 31-37
DOI
NAID
ISSN
0040-8727, 1349-3329
Related Report
Peer Reviewed / Open Access
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[Journal Article] Porphyromonas pogonae sp. nov., an anaerobic but low concentration oxygen adapted coccobacillus isolated from lizards (Pogona vitticeps) or human clinical specimens, and emended description of the genus Porphyromonas Shah and Collins 19882015
Author(s)
Y. Kawamura, S. Kuwabara, S.A. Kania, H. Kato, M. Hamagishi, N. Fujiwara, T. Sato, J. Tomida, K. Tanaka, D.A. Bemis
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Journal Title
Syst. Appl. Microbiol.
Volume: 38
Issue: 2
Pages: 104-109
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Profiling of microbiota from infected root canals with and without marginal leakage using anaerobic culture and molecular techniques (16S rRNA gene sequencing)2015
Author(s)
Sato T, Yamaki K, Kawamura Y, Tomida J, Tian L, Ishida N, Takeuchi Y, Hashimoto K, Abiko Y, Matsuyama J, Takahashi N:
Organizer
The 25th European Congress of Clinical Microbiology and Infectious Diseases
Place of Presentation
Copenhagen, Denmark
Year and Date
2015-04-28
Related Report
Int'l Joint Research
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[Presentation] Porphyromonas pogonae sp. nov., a strong beta-hemolytic, low concentration oxygen tolerarnt species from lizards and human clinical specimens.2014
Author(s)
Kawamura Y, Kuwabara S, Kania SA, Kato H, Hamagishi M, Hayakawa S, Fujiwara N, Naka T, Sato T, Tomida J, Tanaka K, Yoshida Y, Morita Y, Bemis DA.
Organizer
The 114th General Meetings of ASM
Place of Presentation
Boston, USA
Year and Date
2014-05-20
Related Report
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[Book] Oral microbiota in crevices around dental implants: profiling of oral biofilm. In: Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent Interface.2015
Author(s)
Sato T, Kawamura Y, Yamaki K, Ishida N, Tian L, Takeuchi Y, Hashimoto K, Abiko Y, Mayanagi G, Washio J, Matusyama J, Takahashi N:
Total Pages
351
Publisher
Springer
Related Report
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[Book] Sasaki・Suzuki・Takahashi Eds. Interface Oral Health Science 2014. Innovative research on biosis-abiosis intelligent interface.2015
Author(s)
Sato T, Kawamura Y, Yamaki K, Ishida N, Takeuchi Y, Hashimoto K, Abiko Y, Mayanagi G, Washio J, Matsuyama J, Takahashi N.
Total Pages
351
Publisher
Springer, Tokyo
Related Report