Study of the effects of serine proteases on the cariogenicity of Streptococcus mutans

• Project/Area Number: 25462962
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: OZAKI Kazumi 徳島大学, 大学院医歯薬学研究部, 教授 (90214121)
• Co-Investigator(Kenkyū-buntansha): YUMOTO Hiromichi 徳島大学, 病院, 講師 (60284303) ; HOSOKAWA Yoshitaka 徳島大学, 大学院医歯薬学研究部, 助教 (90346601)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: バイオフィルム / クオラムセンシング / オートインデューサー

Outline of Final Research Achievements

The aim of this study was to evaluate the effect of the enzymes, which digest autoinducers, on the cariogenicity of Streptococcus mutans. Trypsin and rHtrA were tended to inhibit bacterial growth and the adherence of S.mutans to a glass test tube, furthermore, suppress biosynthesis of intra/extra-cellular polysaccharide in comparison with negative control. These results suggested that these enzymes will be able to exhibit antibacterial activities and inhibit biofilm formation by S.mutans.

Research Products

• [Journal Article] IL-4 modulates CCL11 and CCL20 productions from IL-1β-stimulated human periodontal ligament cells (2016)
  • Author(s): Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki, Takashi Matsuo
  • Journal Title: Cellular Physiology and Biochemistry Volume: 38 Issue: 1 Pages: 153-159
  • DOI: 10.1159/000438617
  • NAID: 120006550808
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Calcitriol supressed inflammatory reactions in IL-1β-stimulated human periodontal ligament cells (2015)
  • Author(s): Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki, Takashi Matsuo
  • Journal Title: Inflammation Volume: 38 Issue: 6 Pages: 2252-2258
  • DOI: 10.1007/s10753-015-0209-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] IL-6 trans-signaling enhances CCL20 production from IL-1・-stimulated human periodontal ligament cells (2014)
  • Author(s): Hosokawa Y, Shindo S, Hosokawa I, Ozaki K, Matsuo T
  • Journal Title: Inflammation Volume: 37(2) Issue: 2 Pages: 381-386
  • DOI: 10.1007/s10753-013-9750-8
  • Peer Reviewed
• [Journal Article] IL-22 Enhances CCL20 Production in IL-1β-Stimulated Human Gingival Fibroblasts. (2014)
  • Author(s): Hosokawa Y, Hosokawa I, Shindo S, Ozaki K, Matsuo T.
  • Journal Title: Inflammation Volume: 37 Issue: 6 Pages: 2062-2066
  • DOI: 10.1007/s10753-014-9939-5
  • Peer Reviewed
• [Journal Article] Genipin inhibits MMP-1 and MMP-3 release from TNF-α-stimulated human periodontal ligament cells. (2014)
  • Author(s): Shindo S, Hosokawa Y, Hosokawa I, Ozaki K, Matsuo T.
  • Journal Title: Biochimie Volume: 107 Pages: 391-395
  • DOI: 10.1016/j.biochi.2014.10.008
  • Peer Reviewed
• [Journal Article] Genipin inhibits IL-1・-induced CCL20 and IL-6 production from human periodontal ligament cells (2014)
  • Author(s): Shindo S, Hosokawa Y, Hosokawa I, Ozaki K, Matsuo T
  • Journal Title: Cell Physiol Biochem Volume: 33 Issue: 2 Pages: 357-364
  • DOI: 10.1159/000356675
  • Peer Reviewed
• [Journal Article] TLR3 agonist enhances CC chemokine ligand 20 production in IL-1β-stimulated human gingival fibroblasts. (2013)
  • Author(s): Hosokawa Y, Hosokawa I, Shindo S, Ozaki K, Matsuo T.
  • Journal Title: Cellular Immunology Volume: 283 Issue: 1-2 Pages: 8-11
  • DOI: 10.1016/j.cellimm.2013.05.005
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin-3-gallate inhibits CC chemokine ligand 11 production in human gingival fibroblasts (2013)
  • Author(s): Hosokawa Y, Hosokawa I, Shindo S, Ozaki K, Matsuo T
  • Journal Title: Cell Physiol Biochem Volume: 31 Issue: 6 Pages: 960-967
  • DOI: 10.1159/000350114
  • Peer Reviewed
• [Journal Article] Oral porphyromonas gingivalis translocates to the liver and regulates hepatic glycogen synthesis through the Akt/GSK-3β signaling pathway (2013)
  • Author(s): Ishikawa M, Yoshida K, Okamura H, Ochiai K, Takamura H, Fujiwara N, Ozaki K.
  • Journal Title: Biochimica Biophysica Acta-Molecular Basis of Disease Volume: 1832 Issue: 12 Pages: 2035-2043
  • DOI: 10.1016/j.bbadis.2013.07.012
  • Peer Reviewed
• [Presentation] IL-4がヒト歯根膜由来細胞のCCL11およびCCL20産生に及ぼす影響 (2015)
  • Author(s): 細川義隆，細川育子，新藤 智，尾崎和美，松尾敬志
  • Organizer: 第58回秋季日本歯周病学会学術大会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2015-09-12
• [Presentation] PKRやインフラマソームがP.gingivalisを感染させた骨芽細胞において骨吸収に関連する因子の発現に与える影響 (2015)
  • Author(s): 吉田賀弥，岡村裕彦，尾崎和美
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-11
• [Presentation] IL-4 Modulates Chemokine Productions From IL-1β-stimulated Human Periodontal Ligament Cells. (2015)
  • Author(s): Hosokawa Y., Hosokawa I, Shindo S., Ozaki K., Matsuo T.
  • Organizer: The 2015 IADR/AADR/CADR General Session
  • Place of Presentation: Hynes Convention Center (Boston, USA)
  • Year and Date: 2015-03-12
• [Presentation] Melatonin Inhibits Inflammatory Mediators Productions in Human Periodontal Ligament Cells. (2015)
  • Author(s): Hosokawa I, Hosokawa Y., Shindo S., Ozaki K., Matsuo T.
  • Organizer: The 2015 IADR/AADR/CADR General Session
  • Place of Presentation: Hynes Convention Center (Boston, USA)
  • Year and Date: 2015-03-12
• [Presentation] Shikoninがヒト歯根膜由来細胞のIL-6およびIL-8産生に与える影響 (2014)
  • Author(s): 新藤 智，細川 義隆，細川 育子，尾崎 和美，松尾 敬志
  • Organizer: 第141回日本歯科保存学会2014年度秋季学術大会
  • Place of Presentation: 山形テルサ（山形県山形市）
  • Year and Date: 2014-10-31
• [Presentation] GenipinはE.coli LPSが誘導するヒト歯根膜細胞のIL-6およびIL-8産生を抑制する (2014)
  • Author(s): 新藤 智，細川 義隆，細川 育子，尾崎 和美，松尾 敬志
  • Organizer: 第140回日本歯科保存学会2014年度春季学術大会
  • Place of Presentation: 滋賀県立芸術劇場びわ湖ホール（滋賀県大津市）
  • Year and Date: 2014-06-20
• [Presentation] 活性型ビタミンDはIL-1β刺激ヒト歯根膜細胞の炎症性サイトカイン産生を抑制する (2014)
  • Author(s): 細川 義隆，細川 育子，新藤 智，尾崎 和美，松尾 敬志
  • Organizer: 第140回日本歯科保存学会2014年度春季学術大会
  • Place of Presentation: 滋賀県立芸術劇場びわ湖ホール（滋賀県大津市）
  • Year and Date: 2014-06-20
• [Presentation] genipinはTNF-αが誘導するヒト歯根膜由来細胞のIL-6産生を抑制する (2014)
  • Author(s): 北中祐太郎，細川義隆，細川育子，新藤 智，尾崎和美，松尾敬志
  • Organizer: 第57回春季日本歯周病学会学術大会
  • Place of Presentation: 長良川国際会議場（岐阜県岐阜市）
  • Year and Date: 2014-05-23
• [Presentation] IL-6はIL-1βが誘導するヒト歯根膜由来細胞のCCL20産生を増強する (2013)
  • Author(s): 細川義隆，細川育子，新藤 智，尾崎和美，松尾敬志
  • Organizer: 第56回春季日本歯周病学会学術大会
  • Place of Presentation: タワーホール船堀（東京都）
• [Presentation] GenipinはIL-1betaが誘導するヒト歯根膜細胞のCCL20およびIL-6産生を抑制する (2013)
  • Author(s): 新藤 智，細川義隆，細川育子，尾崎和美，松尾敬志
  • Organizer: 第139回日本歯科保存学会2013年度秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館（秋田県）