Suppression of low-level lasser irradiation on gene expression in rheumatoid arthritis

• Project/Area Number: 25462970
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Nihon University
• Principal Investigator: KUBOYAMA Noboru 日本大学, 松戸歯学部, 教授 (10102576)
• Co-Investigator(Kenkyū-buntansha): BHAWAL Ujjal 日本大学, 松戸歯学部, 助教 (50433339) ; ABIKO Yoshimitsu 日本大学, 松戸歯学部, 教授 (70050086)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 低出力レーザー照射 / 関節リウマチ / 骨梁体積率 / 実験動物用マイクロX線CT / リウマチ発症因子 / 骨吸収 / 転写因子 / 海綿骨 / 遺伝子 / 保存修復

Outline of Final Research Achievements

Rheumatoid arthritis (RA) is an inflammatory joint disorder, the progression of which leads to the destruction of cartilage and bone. Low-level laser irradiation (LLLI) and light-emitting diodes irradiation (LEDI) are currently being evaluated for the treatment of collagen-induced RA (CIA) rats. CIA caused swelling of rat joints, and LLLI significantly decreased the swelling, and LLLI and LEDI significantly decreased the CXCL13, MMP3 mRNA level. Micro-CT assessment of LEDI on new bone formation in an experimental rat calvaria periosteal elevation model. LEDI increased BMC, and BV/TV in LED-treated new bone formation of the inner cavity of the PTFE ring compared with controls. A single intraperitoneal injection of streptozotocin was administered to develop a diabetic rat model. The LLLI-induced anti-inflammatory effect was associated with up-regulation of HMGB1expression and function.

Research Products

• [Journal Article] 骨膜挙上モデルラットに対するLED照射による新生骨形成のMicro-CT 解析 (2014)
  • Author(s): 久保山昇，U. K. Bhawal，内田僚一郎，金田明，安孫子宜光
  • Journal Title: 日本レーザー医学誌 Volume: 35(2) Pages: 151-157
  • Peer Reviewed / Open Access
• [Journal Article] Reduction of IL-20 Expression in Rheumatoid Arthritis by Linear Polarized Infrared Light Irradiation (2014)
  • Author(s): A Imaoka, L Zhang, N Kuboyama，Y Abik
  • Journal Title: Laser Therapy Volume: 23(2) Pages: 109-114
  • NAID: 130004872616
  • Peer Reviewed / Open Access
• [Journal Article] Anti-inflammatory activities of light emitting diode irradiation on collagen-induced arthritis in mice (2014)
  • Author(s): N Kuboyama, M Ohta, Y Sato, Y Abiko
  • Journal Title: Laser Therapy Volume: 23(3) Pages: 191-199
  • NAID: 10030782769
  • Peer Reviewed / Open Access
• [Presentation] DNA microarray and microRNA analysis in low-level laser therapy (LLLT)-treated diabetic rat salivary glands (2015)
  • Author(s): Bhawal UK, Fukuoka CY, Simões A, Kuboyama N
  • Organizer: The 1st IDR
  • Place of Presentation: 国立京都国際会館（京都府・京都市）
  • Year and Date: 2015-10-26
  • Int'l Joint Research
• [Presentation] Low-level laser therapy (LLLT) reduced diabetes-induced inflammation in rat salivary glands (2014)
  • Author(s): UK Bhawal , CY Fukuoka, A Simoes, N kuboyama
  • Organizer: 33rd Korea IADR
  • Place of Presentation: Korea
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] LLLT attenuates inflammation in diabetic rat salivary glands signaling and correlations with LLLT (2014)
  • Author(s): Bhawal UK, Fukuoka CY, Simões A, Kuboyama N
  • Organizer: 102th FDI
  • Place of Presentation: India
  • Year and Date: 2014-09-10 – 2014-09-13
• [Presentation] 低出力レーザー照射による実験的関節リウマチに対する治療効果 (2014)
  • Author(s): 久保山 昇
  • Organizer: 第26回日本レーザー治療学会・シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2014-06-28 – 2014-06-29
  • Invited