Development of noninvasive in vivo hard tissue imaging system using photoacoustic microscope

• Project/Area Number: 25462982
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tohoku University
• Principal Investigator: Hatori Kouki 東北大学, 歯学研究科(研究院), 助教 (40372320)
• Co-Investigator(Kenkyū-buntansha): SAIJO Yoshifumi 東北大学, 大学院医工学研究科, 教授 (00292277) ; SASAKI Keiichi 東北大学, 大学院歯学研究科, 教授 (30178644) ; HAGIWARA Yoshihiro 東北大学, 大学院医学系研究科, 准教授 (90436139)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 光音響顕微鏡 / 光音響効果 / 硬組織イメージング / 非侵襲 / in vivo / 血液循環 / 組織弾性 / 光音響信号 / 赤血球 / 片側性咬合支持 / 咬筋 / 超音波信号 / イメージング装置 / 片側咬合支持 / 下顎頭軟骨 / 線維層 / 軟骨下骨 / ラット / 顎関節症 / 下顎頭

Outline of Final Research Achievements

Male Wistar rats of 20-week-old (n=5) were used in this study. Mandibles were resected and fixed. Mandibular condylar cartilage was observed with photoacoustic microscopy (PAS). PAS visualized the lesion from subchondral bone to cancellous bone. Ultrasound microscopy visualized the whole cartilage lesion. Thus PAS could visualized the 3-dimentional color image of from cartilage to cancellous bone.
Male Wistar rats of 16-week-old (n=5) were used in this study. Right three molars of maxilla were extracted. After 4 weeks of extraction, both masseter muscles were resected and fixed. Masseter muscle was observed with PAS. Photoacoustic signal was lower in extracted side than in non-extracted side. Ultrasound signal was higher in non^extracted side than in non-extracted side.

Research Products

• [Journal Article] Acoustic Impedance Microscope for Imaging Human Carious Dentin (2015)
  • Author(s): K. Hatori, Y. Naganuma, Y. Saijo, Y. Hagiwara, M. Iikubo, K. Igari, K. Sasaki
  • Journal Title: 12th Western Pacific Acoustics Conference 2015 Volume: 1 Pages: 1-4
  • Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Acoustic diagnosis device for dentistry (2016)
  • Author(s): Kouki Hatori
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Symposium The 6th International Symposium for Interface Oral Health Science
  • Place of Presentation: Gonryo Kaikan, Sendai, Japan
  • Year and Date: 2016-01-18
  • Int'l Joint Research / Invited
• [Presentation] Acoustic Impedance Microscope for Imaging Human Carious Dentin (2015)
  • Author(s): K. Hatori, Y. Naganuma, Y. Saijo, Y. Hagiwara, M. Iikubo, K. Igari, K. Sasaki
  • Organizer: 12th Western Pacific Acoustics Conference 2015
  • Place of Presentation: Grand Copthorne Waterfront Hotel, Singapore
  • Year and Date: 2015-12-06
  • Int'l Joint Research
• [Presentation] 光音響超音波顕微鏡を利用した片側咬合支持モデルラットにおける咬筋の経時的変化 (2015)
  • Author(s): 羽鳥弘毅、西條芳文、飯久保正弘、萩原嘉廣、佐々木啓一
  • Organizer: 公益社団法人日本補綴歯科学会東北・北海道支部総会・学術大会
  • Place of Presentation: 岩手県歯科医師会館8020プラザ
  • Year and Date: 2015-10-24
• [Presentation] 光音響顕微鏡によるラット咬筋の評価 (2014)
  • Author(s): 山崎玲奈，羽鳥弘毅，飯久保正弘，萩原嘉廣，佐々木啓一，西條芳文
  • Organizer: 第48回日本生体医工学会東北支部大会
  • Place of Presentation: 東北大学工学部青葉記念会館（宮城）
  • Year and Date: 2014-12-06
• [Presentation] The Application of Ultrasound Microscope for Human Caries Dentin (2014)
  • Author(s): K. HATORI, Y. NAGANUMA, Y. SAIJO, Y. HAGIWARA, Y. SHIMIZU, K. IGARI, K. SASAKI
  • Organizer: The 62nd Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: KKR HOTEL OSAKA, （大阪）
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] Ultrasound Microscope for Investigation of Elasticity of Human Carious Dentin (2014)
  • Author(s): Hatori K, Naganuma Y, Saijo Y, Hagiwara Y, Shimizu Y, Igari K, Sasaki K
  • Organizer: INDONESIAN PROSTHODONTIC SOCIETY and JAPAN PROSTHODONTIC SOCIETY JOINT MEETING
  • Place of Presentation: Grand Nikko, Nusa Dua, Bali, (Indonesia)
  • Year and Date: 2014-10-30 – 2014-11-01
• [Presentation] 光音響イメージングを利用したラット下顎頭の可視化 (2014)
  • Author(s): 羽鳥弘毅、佐藤みか、西條芳文、萩原嘉廣、冨士岳志、髙橋健太、佐々木啓一
  • Organizer: 平成25年度第4回光超音波画像研究会
  • Place of Presentation: 産業技術総合研究所つくば東事業所第１会議室（つくば市、茨城県）
• [Presentation] Ultrasound Microscopic Imaging of Human Caries Dentin (2014)
  • Author(s): Yukihiro Naganuma, Kouki Hatori, Yoshifumi Saijo, Yoshihiro Hagiwara, Yoshinaka Shimizu, Kazuko Igari, Keiichi Sasaki
  • Organizer: East Asisn Consortium on Biomedical Engineering 8th Student Workshop
  • Place of Presentation: Aoba Memorial Hall, Aobayama Campus, Tohoku University, Sendai, Japan