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Development of micro/nano-patterned sheet for periodontal tissue regeneration aimed at 3d tissue construction

Research Project

Project/Area Number 25463047
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dental engineering/Regenerative dentistry
Research InstitutionHokkaido University

Principal Investigator

Akasaka Tsukasa  北海道大学, 歯学研究院, 准教授 (00360917)

Project Period (FY) 2013-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsマイクロ・ナノパターン / ナノインプリント / ゼラチン / アパタイト / コンポジットレジン / 歯周組織再生 / 歯根膜線維芽細胞 / 骨芽細胞様細胞 / 破骨細胞 / 微細構造 / コラーゲン / 歯科材料 / パターニング / 歯根膜再生 / 細胞応答
Outline of Final Research Achievements

Preparation of micro/nano-patterned sheets was investigated by nanoimprinting with collagen or apatite materials for periodontal tissue regeneration. Micro/nano patterned gelation can be obtained by thermal crosslinking or crosslinking with genipin. The apatite or composite resin patterns were obtained by using appropriate materials and molds. As a result of cell assay, the patterns largely influenced cell adhesion, proliferation, and alignment.

Report

(6 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (34 results)

All 2018 2017 2016 2015 2014 2013 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (20 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (7 results)

  • [Journal Article] Effect of a nano-scale fine hole pattern on the differentiation of RAW264.7 cells into osteoclasts2018

    • Author(s)
      R. TAKATA, T. AKASAKA, M. TAMAI, Y. YOSHIMURA, T. TAIRA, H. MIYAJI, Y. TAGAWA, S. YAMAGATA, J. IIDA, Y. YOSHIDA
    • Journal Title

      Digest Journal of Nanomaterials and Biostructures

      Volume: 13(2)

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preparation of micro/nano-patterned gelatins crosslinked with genipin2018

    • Author(s)
      Reika Makita, Tsukasa Akasaka, Seiichi Tamagawa, Yasuhiro Yoshida, Saori Miyata, Hirofumi Miyaji, and Tsutomu Sugaya
    • Journal Title

      Beilstein Journal of Nanotechnology

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of Micro/Nano-Patterned Surfaces on Cell Adhesion of Ca9-22 cells2017

    • Author(s)
      Kaga N, Horiuchi R, Yokoyama A, Akasaka T, Yoshida Y
    • Journal Title

      e-Journal of Surface Science and Nanotechnology

      Volume: 15 Issue: 0 Pages: 1-6

    • DOI

      10.1380/ejssnt.2017.1

    • NAID

      130005394689

    • ISSN
      1348-0391
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] SUBMICRO-PATTERNING OF CURABLE DENTAL MATERIALS BY MOLDING METHODS : A SCREENING TRIAL2017

    • Author(s)
      Akasaka, T Miyaji, H Imamura, T Kaga, N Yokoyama, A Yoshida, Y
    • Journal Title

      Digest Journal of Nanomaterials and Biostructures

      Volume: 12 Pages: 281-292

    • NAID

      120006024408

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Adhesion of Osteoblast-like Cells (Saos-2) on Micro-/Submicro-Patterned Apatite Scaffolds Fabricated with Apatite Cement Paste by Micro-Molding2016

    • Author(s)
      T Akasaka, H Miyaji, N Kaga, A Yokoyama, S Abe, Y Yoshida
    • Journal Title

      Nano Biomedicine

      Volume: 8 Issue: 2 Pages: 112-122

    • DOI

      10.11344/nano.8.112

    • NAID

      130005243593

    • ISSN
      1883-5198, 2185-4734
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adhesion of Human Osteoblast-like Cells (Saos-2 cells) on Micro/nanopatterned Structures Sputter-Coated with Titanium2016

    • Author(s)
      Kaga, Naoyuki Akasaka, Tsukasa Horiuchi, Rumi Yoshida, Yasuhiro Yokoyama, Atsuro
    • Journal Title

      Nano Biomedicine

      Volume: 8 Issue: 2 Pages: 74-82

    • DOI

      10.11344/nano.8.74

    • NAID

      130005243546

    • ISSN
      1883-5198, 2185-4734
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of Protein Adsorption on Alignment of Human Gingival Fibroblasts on Grooved Composite Resin2016

    • Author(s)
      T Akasaka, T Imamura, H Miyaji, N Kaga, A Yokoyama, Y Yoshida
    • Journal Title

      e-Journal of Surface Science and Nanotechnology

      Volume: 14 Issue: 0 Pages: 225-230

    • DOI

      10.1380/ejssnt.2016.225

    • NAID

      130005433502

    • ISSN
      1348-0391
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] キャピラリーフォースによるマイクロピラー自己組織化による培養担体の調製2018

    • Author(s)
      赤坂 司,玉井美保,阿部薫明,吉田靖弘
    • Organizer
      第71回日本歯科理工学会学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 硬化可能な歯科材料によるマイクロ・ナノパターニング2017

    • Author(s)
      赤坂 司,加我公行,横山敦郎,阿部薫明,吉田靖弘
    • Organizer
      日本歯科理工学会 第67回 春期学術講演会
    • Place of Presentation
      日本歯科大学生命歯学部富士見ホール
    • Year and Date
      2017-04-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 硬化可能な歯科材料によるマイクロ・ナノパターニング2017

    • Author(s)
      赤坂 司,加我公行,横山敦郎,阿部薫明,吉田靖弘
    • Organizer
      第69回日本歯科理工学会学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲニピン架橋によるマイクロ・ナノパターン化ゼラチンの作製2017

    • Author(s)
      赤坂 司,阿部薫明,吉田靖弘
    • Organizer
      第70回日本歯科理工学会学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マイクロパターンによる歯根膜線維芽細胞の細胞集合2017

    • Author(s)
      赤坂 司,加我公行,横山敦郎,阿部薫明,吉田靖弘
    • Organizer
      日本バイオマテリアル学会 北海道ブロック第2回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cell aggregation of periodontal ligament fibroblasts controlled by micro-patterns2016

    • Author(s)
      Akasaka T, Kaga N, Yokoyama A, Abe S, Yoshida Y
    • Organizer
      国際歯科材料会議 2016 第68回 秋期学術講演会
    • Place of Presentation
      ストーンホテル,バリ,インドネシア
    • Year and Date
      2016-11-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The effect of protein adsorption on cell alignment of human gingival fibroblast on the grooved composite resins2016

    • Author(s)
      Tsukasa Akasaka, Hirofumi Miyaji, Naoyuki Kaga, Atsuro Yokoyama, Yasuhiro Yoshida
    • Organizer
      AsiaNano 2016
    • Place of Presentation
      Sapporo Convention Center
    • Year and Date
      2016-10-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] マイクロパターンによる歯根膜線維芽細胞の細胞集合2016

    • Author(s)
      赤坂 司,加我公行,横山敦郎,阿部薫明,吉田靖弘
    • Organizer
      日本歯科理工学会 第69回 春期学術講演会
    • Place of Presentation
      九州大学医学部百年講堂
    • Year and Date
      2016-04-16
    • Related Report
      2016 Research-status Report
  • [Presentation] マイクロパターンにおける歯根膜線維芽細胞の細胞配向および移動2015

    • Author(s)
      赤坂 司,加我公行,横山敦郎,阿部薫明,吉田靖弘
    • Organizer
      第67回日本歯科理工学会学術講演会
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] 歯表面での3D微細構造構築によるバイオミメティック次世代歯科治療へ向けて2015

    • Author(s)
      赤坂 司、加我公行、宮治裕史、横山敦郎、吉田靖弘
    • Organizer
      第31回「歯科医学を中心とした総合的な研究を推進する集い」
    • Place of Presentation
      歯科医師会館
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] 歯の上でナノテク? マイクロ・ナノパターンの歯科治療応用へ向けて2015

    • Author(s)
      赤坂 司
    • Organizer
      第1回北海道ナノバイオ研究会シンポジウム
    • Place of Presentation
      北海道大学医学部
    • Year and Date
      2015-08-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 赤坂 司,加我公行,横山敦郎,阿部薫明,吉田靖弘2015

    • Author(s)
      マイクロパターンによる歯根膜線維芽細胞の細胞集合
    • Organizer
      第68回日本歯科理工学会学術講演会
    • Place of Presentation
      九州大学医学部百年講堂
    • Year and Date
      2015-04-16
    • Related Report
      2015 Research-status Report
  • [Presentation] マイクロ・ナノパターン化材料に対する細胞接着挙動2015

    • Author(s)
      赤坂 司,加我公行,阿部薫明,吉田靖弘
    • Organizer
      第66回日本歯科理工学会学術講演会
    • Place of Presentation
      仙台市情報・産業プラザ
    • Year and Date
      2015-04-11
    • Related Report
      2015 Research-status Report
  • [Presentation] 歯の表面で微細構造をつくる:歯科治療応用を目指した微細構造への細胞接着の応用2015

    • Author(s)
      赤坂 司
    • Organizer
      日本機械学会北海道支部バイオメカニクス懇話会
    • Place of Presentation
      北海道大学(札幌市北区)
    • Year and Date
      2015-02-19
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 再生医療に繋がるバイオ系マイクロ・ナノパターンの作製と細胞応答2014

    • Author(s)
      赤坂 司
    • Organizer
      文部科学省ナノテクプラットフォーム H26地域セミナー
    • Place of Presentation
      道立総合研究プラザ(札幌市北区)
    • Year and Date
      2014-10-31
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] マイクロ・ナノパターン化コンポジットレジンへの細胞接着に対する細胞種の影響2014

    • Author(s)
      赤坂 司、加我公行、阿部薫明、吉田靖弘
    • Organizer
      第64回日本歯科理工学会
    • Place of Presentation
      アステールプラザ(広島県中区)
    • Year and Date
      2014-10-04 – 2014-10-05
    • Related Report
      2014 Research-status Report
  • [Presentation] マイクロ・ナノパターン化コンポジットレジンへの細胞接着2014

    • Author(s)
      赤坂 司
    • Organizer
      第63回日本歯科理工学会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2014-04-12 – 2014-04-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Cell adhesion of osteoblast-like cells on micro/nano-patterned apatite scaffolds2013

    • Author(s)
      Tsukasa Akasaka, Fumio Watari
    • Organizer
      Seventh international conference on science and technology of advanced ceramics
    • Place of Presentation
      Mielparque-yokoyama, Yokohama
    • Related Report
      2013 Research-status Report
  • [Presentation] マイクロ・ナノパターン化バイオマテリアルの作製2013

    • Author(s)
      赤坂 司、亘理文夫
    • Organizer
      平成25年度歯科理工学会北海道・東北支部 夏季セミナー
    • Place of Presentation
      岩手県歯科医師会館、岩手
    • Related Report
      2013 Research-status Report
  • [Presentation] マイクロ・ナノパターン化したアパタイト担体への細胞接着2013

    • Author(s)
      赤坂 司、亘理文夫
    • Organizer
      第62回日本歯科理工学会
    • Place of Presentation
      日本歯科大学 新潟生命歯学部校舎、新潟
    • Related Report
      2013 Research-status Report
  • [Remarks] 研究内容(赤坂 司)

    • URL

      https://www.den.hokudai.ac.jp/seitaizairyou/akasaka/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 北海道大学 研究者総覧

    • URL

      https://researchers.general.hokudai.ac.jp/profile/ja.pGAj0CcQow4x8PHcQnHJzQ==.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/read0103543/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 個人研究内容

    • URL

      https://www.den.hokudai.ac.jp/seitaizairyou/akasaka/

    • Related Report
      2016 Research-status Report
  • [Remarks] Research map

    • URL

      http://researchmap.jp/read0103543/

    • Related Report
      2016 Research-status Report
  • [Remarks] Google scahlor

    • Related Report
      2016 Research-status Report
  • [Remarks] Huscap

    • URL

      http://eprints.lib.hokudai.ac.jp/statistics/statslist.php?statsurl=q2aRK20b7gIMYtP

    • Related Report
      2016 Research-status Report

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Published: 2014-07-25   Modified: 2019-12-27  

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