Analysis of expression pattern and function of micro-RNA in craniosynostosis

• Project/Area Number: 25463168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KOBAYASHI YUKIHO 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (20596233)
• Co-Investigator(Kenkyū-buntansha): MORIYAMA Keiji 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (20262206)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 頭蓋冠縫合部早期癒合症 / miRNA / アペール症候群 / 頭蓋冠冠状縫合部 / 歯科矯正学 / microRNA / 先天性疾患 / Craniosynostosis / FGFR / FGF

Outline of Final Research Achievements

We analyzed the expression pattern of miRNA in the calvarial sutures of Apert syndrome model mice (Ap mice) and the control mice (Ct mice). Ap mice showed higher expression of 19 kinds of miRNA and lower 5 kinds of miRNA significantly. Additionally, mmu-miR-182-5p and mmu-miR-206-3p significantly highly expressed in Ap mice by real-time PCR.

Research Products

• [Journal Article] Therapeutic Effect of Nanogel-Based Delivery of Soluble FGFR2 with S252W Mutation on Craniosynostosis (2014)
  • Author(s): M. Yokota, Y. Kobayashi, J. Morita, H. Suzuki, Y. Hashimoto, Y. Sasaki, K. Akiyoshi, K. Moriyama
  • Journal Title: PLOS ONE Volume: 9 Issue: 7 Pages: e101693-e101693
  • DOI: 10.1371/journal.pone.0101693
  • Peer Reviewed / Open Access
• [Journal Article] RELAXIN enhances differentiation and matrix mineralization through Relaxin/insulin-like family peptide receptor 2 (Rxfp2) in MC3T3-E1 cells in vitro. (2014)
  • Author(s): Duarte C, Kobayashi Y, Kawamoto T, Moriyama K
  • Journal Title: Bone Volume: 65 Pages: 92-101
  • DOI: 10.1016/j.bone.2014.05.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Long-term orthodontic and surgical treatment and stability of a patient with Beckwith-Wiedemann syndrome. (2014)
  • Author(s): Hikita R, Kobayashi Y, Tsuji M, Kawamoto T, Moriyama K.
  • Journal Title: Am J Orthod Dentofacial Orthop. Volume: 145 Issue: 5 Pages: 672-684
  • DOI: 10.1016/j.ajodo.2013.08.019
  • Peer Reviewed / Open Access
• [Journal Article] Soluble form of FGFR2 with S252W partially prevents craniosynostosis of the apert mouse model (2014)
  • Author(s): Morita J, Nakamura M, Kobayashi Y, Deng CX, Funato N, Moriyama K
  • Journal Title: Dev Dyn Volume: 243(4) Issue: 4 Pages: 560-567
  • DOI: 10.1002/dvdy.24099
  • Peer Reviewed
• [Journal Article] Stability of mRNA influences steoporotic bone mass via CNOT3 (2014)
  • Author(s): Watanabe, C. Morita, M. Yamamoto T. (13名)
  • Journal Title: Natl Acad Sci U S A Volume: Vol.111 Issue: 7 Pages: 2692-2697
  • DOI: 10.1073/pnas.1316932111
  • Peer Reviewed / Open Access
• [Journal Article] Long-term orthodontic and surgical (2014)
  • Author(s): Hikita R, Kobayashi Y, Tsuji M, Kawamoto T, Moriyama K.
  • Journal Title: Am J Orthod Dentofacial Orthop. Volume: 未定
  • Peer Reviewed
• [Presentation] マウス頭蓋矢状縫合部側方拡大時の骨リモデリングに対する子宮弛緩因子リラキシンの影響 (2014)
  • Author(s): ドゥアルテ カロリーナ、小林 起穂、森田 淳平、川元 龍夫、森山 啓司
  • Organizer: 第73回日本矯正歯科学会大会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2014-10-20 – 2014-10-22
• [Presentation] Relaxin Affects Differentiation and Mineralization of MC3T3-E1 Cells Through Rxfp2. (2013)
  • Author(s): Duarte C, Kobayashi Y, Kawamoto T, Moriyama K.
  • Organizer: 2nd Joint Annual Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research
  • Place of Presentation: 兵庫県 神戸市 神戸コンベンションセンター
• [Presentation] Soluble fibroblast growth factor receptor 2 with Apert mutation inhibits differentiation and mineralization of osteoblast. (2013)
  • Author(s): Yoshizaki M, Kobayashi Y, Moriyama K.
  • Organizer: 2nd Joint Meeting of the International Bone Mineral Society and The Japanese Society for Bone and Mineral Research
  • Place of Presentation: 兵庫県 神戸市 神戸コンベンションセンター
• [Presentation] 子宮弛緩因子リラキシンが骨芽細胞の形質に及ぼす影響についての検討. (2013)
  • Author(s): ドゥアルテ カロリーナ、小林起穂、川元龍夫、森山啓司.
  • Organizer: 第72回日本矯正歯科学会大会
  • Place of Presentation: 長野県 松本市 キッセイ文化ホール
• [Presentation] 頭蓋冠縫合部早期癒合症に対するFGF/FGFRシクナルを標的とした新規治療法開発への試み. (2013)
  • Author(s): 吉崎正子、小林起穂、佐々木善浩、秋吉一成、森山啓司.
  • Organizer: 第72回日本矯正歯科学会大会
  • Place of Presentation: 長野県 松本市 キッセイ文化ホール