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Cryopreservation of rat MSCs by use of a programmed freezer with magnetic field.

Research Project

Project/Area Number 25463179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthodontics/Pediatric dentistry
Research InstitutionHiroshima University

Principal Investigator

Kaku Masato  広島大学, 大学病院, 講師 (10325194)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords骨髄間葉系幹細胞 / 凍結保存 / 磁場 / 細胞移植 / 骨再生 / 間葉系幹細胞 / プログラムフリーザー / 再生医療 / MSC
Outline of Final Research Achievements

Survival and proliferation rates of MSCs were significantly higher in CAS freezer than in the non-magnetic freezer. Alizarin positive reaction, large amount of calcium quantification, and greater alkaline phosphatase activity were shown in both the non-cryopreserved and CAS groups after osteogenic differentiation. Moreover, Oil Red O staining positive reaction and high amount of PPARγ and FABP4 mRNAs were shown in both the non-cryopreserved and CAS groups after adipogenic differentiation.
Animal studies indicate that MSCs survival and proliferation rates were significantly higher in the CAS group than in the Direct group. In the Control and CAS groups, a large amount of new bone formation and a suture-like gap was identified 24 weeks after transplantation, whereas only a small amount of new bone formation was observed in the Direct group.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Cranial suture-like gap and bone regeneration after transplantation of cryopreserved MSCs by use of a programmed freezer with magnetic field in rats.2015

    • Author(s)
      Kojima S-I., Kaku M., Kawata T., Motokawa M., Sumi H., Shikata H., Abonti TR., Kojima S-T., Yamamoto T., Tanne K., Tanimoto K.
    • Journal Title

      Cryobiology

      Volume: 70 Issue: 3 Pages: 262-268

    • DOI

      10.1016/j.cryobiol.2015.04.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cranial bone regeneration after cranioplasty using cryopreserved autogenous bone by a programmed freezer with a magnetic in rats.2014

    • Author(s)
      Kaku M., Koseki H., Kojima S-I., Sumi H., Shikata H., Kojima S-T., Motokawa M., Fujita T., Tanimoto K., Tanne K.
    • Journal Title

      Cryo letters

      Volume: 35

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation of rat MSCs by use of a programmed freezer with magnetic field.2013

    • Author(s)
      2: Kojima S., Kaku M., Kawata T., Sumi H., Shikata H., Abonti TR., Kojima S-T., Fujita T., Motokawa M., Tanne K.
    • Journal Title

      Cryobiology

      Volume: 67 Issue: 3 Pages: 258-263

    • DOI

      10.1016/j.cryobiol.2013.08.003

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] A case of autotransplantation of a cryopreserved tooth with magnetic field programmed freezer.2015

    • Author(s)
      Shikata H., Kaku M., Motokawa M., Kojima S., Sumi H., Kojima S., Tanne K., Tanimoto K.
    • Organizer
      American association of orthodontists meeting
    • Place of Presentation
      SanFrancisco (USA)
    • Year and Date
      2015-05-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 7.ラット正中矢状縫合部骨欠損へ移植した凍結骨髄由来間葉系幹細胞の組織再生誘導能2014

    • Author(s)
      小島俊逸, 加来真人, 小島将督, 谷本幸太郎
    • Organizer
      第38回日本口蓋裂学会
    • Place of Presentation
      札幌市 札幌コンベンションセンター
    • Year and Date
      2014-05-29 – 2014-05-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 1. 磁場を利用したラット骨髄由来間葉系幹細胞の長期凍結保存法の確立2013

    • Author(s)
      小島俊逸, 加来真人, 河田俊嗣, 本川雅英, 藤田 正, 大谷淳二, 角 明美, 四方花佳, 丹根一夫
    • Organizer
      第46回広島大学歯学会総会
    • Place of Presentation
      広島市
    • Related Report
      2013 Research-status Report
  • [Presentation] 7. 磁場を利用したラット骨髄由来間葉系幹細胞の長期凍結保存法の確立2013

    • Author(s)
      小島俊逸, 加来真人, 本川雅英, 藤田 正, 角 明美, 四方花佳, 小島将督, 河田俊嗣, 丹根一夫
    • Organizer
      第72回日本矯正歯科学会大会
    • Place of Presentation
      松本市
    • Related Report
      2013 Research-status Report
  • [Presentation] 8. 磁場を利用したラット骨髄由来間葉系幹細胞の長期凍結保存法の確立2013

    • Author(s)
      小島俊逸, 加来真人, 本川雅英, 藤田 正, 角 明美, 四方花佳, Tahsin Raquib Abonti, 小島将督, 谷本幸太郎
    • Organizer
      第52回広島県歯科医学会・第97回広島大学歯学会
    • Place of Presentation
      広島市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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