Elucidation of epigenetic mechanism in gingival epithelium by periodontal pathogen
Project/Area Number |
25463223
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Periodontology
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Research Institution | Health Sciences University of Hokkaido |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
太田 亨 北海道医療大学, 個体差健康科学研究所, 教授 (10223835)
荒川 俊哉 北海道医療大学, 歯学部, 准教授 (40306254)
古市 保志 北海道医療大学, 歯学部, 教授 (80305143)
安彦 善裕 北海道医療大学, 歯学部, 教授 (90260819)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | エピジェネティクス / 歯周疾患 / 線維芽細胞 / DNAメチル化 / LPS / 歯周病原菌 / 歯周組織 / P.gingivalis / 歯周病 / 歯肉線維芽細胞 / 歯肉上皮 |
Outline of Final Research Achievements |
In this study, we investigated epigenetics change when P. gingivalis-derived LPS was allowed to act on human gingival fibroblasts for a long time. LPS chronic stimulation was given to human gingival fibroblasts, DNA hypermethylation and mRNA expression decrease of fibronectin type III (FN), αI (XII) collagen (XII-collagen) and extracellular matrix protein related genes were observed . Protein analysis also reduced the expression of XII-collagen and FN and its expression was restored by demethylating agent.These result suggest that periodontal pathogen-derived LPS is involved in the pathogenesis and progression of periodontal disease due to gene expression reduction through epigenetic modification.
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Report
(5 results)
Research Products
(43 results)
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[Journal Article] Immunohistochemical evaluation of Klotho and DNA methyltransferase 3a in oral squamous cell carcinomas.2017
Author(s)
Adhikari BR, Uehara O, Matsuoka H, Takai R, Harada F, Utsunomiya M, Chujo T, Morikawa T, Shakya M, Yoshida K, Sato J, Arakawa T, Nishimura M, Nagayasu H, Chiba I, Abiko Y.
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Journal Title
Med Mol Morphol.
Volume: -
Issue: 3
Pages: 155-160
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Osseous choristoma of the tongue: two case reports.2016
Author(s)
Bhoj Raj Adhikari, Jun Sato, Tetsuro Morikawa, June Obara-Itoh, Masafumi Utsunomiya, Fumiya Harada, Takatoshi Chujo, Rie Takai, Koki Yoshida, Michiko Nishimura, Mamata Shakya, Hiroki Nagayasu, Yoshihiro Abiko.
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Journal Title
J.Medical Case Reports.
Volume: 10
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] HBD-2 is downregulated in oral carcinoma cells by DNA hypermethylation, and increased expression of hBD-2 by DNA demethylation and gene transfection inhibits cell proliferation and invasion.2014
Author(s)
Kamino Y, Kurashige Y, Uehara O, Sato J, Nishimura M, Yoshida K, Arakawa T, Nagayasu H, Saitoh M, Abiko Y.
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Journal Title
Oncol Rep.
Volume: 32
Issue: 2
Pages: 462-468
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Polyamines Remove DNA Methylation of COL15A1 Caused by LPS.2017
Author(s)
R. Takai, O. Uehara, T. Morikawa, F. Harada, K. Yoshida, J. Sato, M. Nishimura, I. Chiba, Y. Abiko, T.Ohta.
Organizer
95th IADR
Place of Presentation
Sanfrancisco, USA
Year and Date
2017-03-22
Related Report
Int'l Joint Research
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[Presentation] Epigenetic silencing of anti-aging gene Klotho may laed to hypermethylation of DNMT3a in oral cancer: Immunohistochemical analysis.2017
Author(s)
hoj Raj Adhikari, Hirofumi Matsuoka, Fumiya Harada, Takatoshi Chujo, Tetsuro Morikawa, Puja Neopane, Osamu Uehara, Koki Yoshida, Michiko Nishimura, Itsuo Chiba, Yoshihiro Abiko.
Organizer
第35回北海道医療大学歯学会・学術大会
Place of Presentation
札幌
Related Report
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[Presentation] Expression of Stem Cell Markers in ERM by Epigenetic Agents.2016
Author(s)
Koki Yoshida,Osamu Uehara, Tetsuro Morikawa, Bhoj Adhikari, Fumiya Harada, Takatoshi Chujo, Rie Takai, Jun Sato, Michiko Nishimura, Yoshihiro Abiko.
Organizer
94th IADR
Place of Presentation
Seoul, Korea
Year and Date
2016-06-22
Related Report
Int'l Joint Research
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[Presentation] High Frequency of DNA Hypermethylation in Lichen Planus.2016
Author(s)
Takatoshi Chujo,Bhoj Adhikari, Tetsuro Morikawa, Fumiya Harada, Koki Yoshida, Jun Sato, Michiko Nishimura, Kenichi Matsuzaka, Takashi Inoue, Yoshihiro Abiko.
Organizer
94th IADR
Place of Presentation
Seoul, Korea
Year and Date
2016-06-22
Related Report
Int'l Joint Research
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[Presentation] Genome-wide Analysis of DNA Hypermethylation in Epithelial Cells by Arecoline.2016
Author(s)
Bhoj Adhikari, Osamu Uehara, Koki Yoshida, Tetsuro Morikawa, Fumiya Harada, Takatoshi Chujo, Rie Takai, Jun Sato, Michiko Nishimura, Yoshihiro Abiko.
Organizer
94th IADR
Place of Presentation
Seoul, Korea
Year and Date
2016-06-22
Related Report
Int'l Joint Research
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[Presentation] DNA Hypermethylation in HPDLs Stimulated With LPS for Prolonged Periods.2015
Author(s)
R. Takai, F. Harada, M. Utsunomiya, T. Chujo, O. Uehara, K. Yoshida, J. Sato, M. Nishimura, I. Chiba, Y. Abiko.
Organizer
93rd IADR
Place of Presentation
Boston, USA
Year and Date
2015-03-11 – 2015-03-14
Related Report
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[Presentation] Hypermethylation of XII-collagen and Fibronectin in HPDL cells by LPS.2014
Author(s)
Y. ABIKO1, R. TAKAI1, O. UEHARA2, F. HARADA, M. UTSUNOMIYA, T. CHUJO1, K. YOSHIDA3, J. SATO1, M. NISHIMURA1, and M. SAITOH4.
Organizer
IADR /PERa Congress
Place of Presentation
Dubrovnik, Croatia
Year and Date
2014-09-10 – 2014-09-13
Related Report
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[Presentation] Hypermethylation of Inflammation-related genes in HPDL cells stimulated with LPS.2014
Author(s)
R. TAKAI1, F. HARADA, M. UTSUNOMIYA, T. CHUJO1, O. UEHARA2, K. YOSHIDA3, J. SATO1, M. NISHIMURA1, I. CHIBA4., Y. ABIKO1
Organizer
IADR /PERa Congress
Place of Presentation
Dubrovnik, Croatia
Year and Date
2014-09-10 – 2014-09-13
Related Report
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