| Project/Area Number |
25463223
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
太田 亨 北海道医療大学, 個体差健康科学研究所, 教授 (10223835)
荒川 俊哉 北海道医療大学, 歯学部, 准教授 (40306254)
古市 保志 北海道医療大学, 歯学部, 教授 (80305143)
安彦 善裕 北海道医療大学, 歯学部, 教授 (90260819)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | エピジェネティクス / 歯周疾患 / 線維芽細胞 / DNAメチル化 / LPS / 歯周病原菌 / 歯周組織 / P.gingivalis / 歯周病 / 歯肉線維芽細胞 / 歯肉上皮 |
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| Outline of Final Research Achievements |
In this study, we investigated epigenetics change when P. gingivalis-derived LPS was allowed to act on human gingival fibroblasts for a long time. LPS chronic stimulation was given to human gingival fibroblasts, DNA hypermethylation and mRNA expression decrease of fibronectin type III (FN), αI (XII) collagen (XII-collagen) and extracellular matrix protein related genes were observed . Protein analysis also reduced the expression of XII-collagen and FN and its expression was restored by demethylating agent.These result suggest that periodontal pathogen-derived LPS is involved in the pathogenesis and progression of periodontal disease due to gene expression reduction through epigenetic modification.
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